Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 124265891

GI number: 124265891

Start: 727937

End: 729541

Strand: Direct

Name: braD [H]

Synonym: Mpe_A0698

Alternate gene names: 124265891

Gene position: 727937-729541 (Clockwise)

Preceding gene: 124265890

Following gene: 124265892

Centisome position: 18.0

GC content: 71.65

Gene sequence:

>1605_bases
TTGAATGCCTTGCGTGCTTTCCTGCTGAGCCTGTGCGCCGCCGGGCTGCTGGGGCTGGCTGCGGCTCCCGCGCGTGCCAT
GACGCCCGCGCAGGCGCTCGCCATCTCGGCCGGCGACACCGACACCCGCGTCGCCGCCCTGCAGCAGGCCATCGCCGCGC
TCGACCCGCGCGTGCCGGTGCTGGTGCAGGCGCTGCTGGACGGTGAGGTGAAGGTCGACGACCAGCGCGCCTACATCGTC
GCGGCCGACGGCTCGGCCGTCGACGCCGCCACCGGCCAGCCGGCCACGCCCCCCGAGACCGCGGAAGACGCGGTCAACAA
CAACCGCATGCGCGGCGAGCTGCAGGCCGCGCTGGCCGCGCTGCAGCTGATCACCGGCGACCTCACGCAACGCCGCGCCG
CCATCGGCTCGCTGCGCGAGGCGATGGACGAGGCCAAGCTGCCGCTCATCGAGCAGGCGCTGGCCAAGGAGGCCGACCCC
GCACTCAAGGCCGAGCTGGAGACGCTGCGCGCCAAGGGCCTGATCGCCTCCACCGACCCGGCCAAGCGCCTGCAGGCCGC
GCAGGCCCTGGCCAGCAGCACCACGCCGGCCACCCGCAGCCTGCTGCTCGAGCGCCTGAAGCCGGCAGCCGAGGGCGGGG
AGGCCGACCCCGCGGTGCGCGCGGCGCTGCAGGCCTCACTCGACGGCGTCAATTCCATGCTGGCCTGGGGCGAACGCGCC
GGCGTCGTGTTCACCGGCATCAGCCTGGGTTCCATCCTGCTGCTGGCGGCGCTGGGCCTGGCCATCACCTACGGGCTGAT
GGGCGTCATCAACATGGCGCACGGCGAGCTCATCATGATCGGCGCCTACGCCACCTACCTCACGCAGAACGCCTTCCGCG
CCTGGCTGCCGGACCACTTCGACTGGTACATCGTCGCGGCCATCCCGCTGTCCTTCCTCGCCGCGGCGGCGGTGGGCGCG
GTGCTGGAGCGCGGCGTCATCCGCTTCCTCTACGGCCGCCCGCTGGAGACGCTGCTCGCCACCTGGGGCATCAGCCTGGT
GCTGATGCAGGGCGTGCGCTCGCTGTTCGGCGCACAGAACGTGCCGGTGGAGAACCCGGCCTGGCTGTCGGGCGGCGTGC
AGATCATGAGCAACCTGGTGCTGCCGTGGAACCGCATCGCCATCGTGGTGTTCGCGGCCATCGTGCTGGGCGGCATCGCC
TTCCTGATCGCGAAGACGCGGCTGGGGCTGTTCGTGCGCAGCGTCACCCAGAACCGCCCCATGGCCTCGTGCATGGGTGT
CAACACCGCGCGCGTCGACACGCTGGCGTTCGCGCTCGGGGCCGGCGTGGCGGGGCTGGCGGGCTGCGCGGTCAGCCAGG
TCGGCAACGTCGGGCCCGACCTGGGGCAGGGCTACATCGTCGACTCCTTCATGGTGGTGGTGCTGGGCGGCGTGGGCCAG
CTCGCCGGCACCGTGTACGCGGCACTGGGGCTGGGCGTGATCAACAAGTTCCTCGAAGGCTGGGCCGGCGCCGTGCTGGC
GAAGATCATCGTGCTGGTGTTCATCGTGGTGTTCATCCAGAAGCGGCCGCAGGGGCTGTTCGCATTGAAGGGGAGAGAGG
CATGA

Upstream 100 bases:

>100_bases
GCGGCGATCGGACTGTCCACCAGGTCGCCGCCGGTCCTGCCGGCGCGCAGCCTTCTTTTTCTTCTTCTCATGGCTTCCTA
CTCTCCACGGTGACGACGAA

Downstream 100 bases:

>100_bases
GTGCCGTGCTGACGCCTCCTCTGCCCGTGGCACCGCCGCGCAGTCGCCTGCTGCAGGGCAAGGCCTGGACGGCGCTGCTG
GCCGCGGTGATCACCGTGAC

Product: putative branched-chain amino acid transport system, permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MNALRAFLLSLCAAGLLGLAAAPARAMTPAQALAISAGDTDTRVAALQQAIAALDPRVPVLVQALLDGEVKVDDQRAYIV
AADGSAVDAATGQPATPPETAEDAVNNNRMRGELQAALAALQLITGDLTQRRAAIGSLREAMDEAKLPLIEQALAKEADP
ALKAELETLRAKGLIASTDPAKRLQAAQALASSTTPATRSLLLERLKPAAEGGEADPAVRAALQASLDGVNSMLAWGERA
GVVFTGISLGSILLLAALGLAITYGLMGVINMAHGELIMIGAYATYLTQNAFRAWLPDHFDWYIVAAIPLSFLAAAAVGA
VLERGVIRFLYGRPLETLLATWGISLVLMQGVRSLFGAQNVPVENPAWLSGGVQIMSNLVLPWNRIAIVVFAAIVLGGIA
FLIAKTRLGLFVRSVTQNRPMASCMGVNTARVDTLAFALGAGVAGLAGCAVSQVGNVGPDLGQGYIVDSFMVVVLGGVGQ
LAGTVYAALGLGVINKFLEGWAGAVLAKIIVLVFIVVFIQKRPQGLFALKGREA

Sequences:

>Translated_534_residues
MNALRAFLLSLCAAGLLGLAAAPARAMTPAQALAISAGDTDTRVAALQQAIAALDPRVPVLVQALLDGEVKVDDQRAYIV
AADGSAVDAATGQPATPPETAEDAVNNNRMRGELQAALAALQLITGDLTQRRAAIGSLREAMDEAKLPLIEQALAKEADP
ALKAELETLRAKGLIASTDPAKRLQAAQALASSTTPATRSLLLERLKPAAEGGEADPAVRAALQASLDGVNSMLAWGERA
GVVFTGISLGSILLLAALGLAITYGLMGVINMAHGELIMIGAYATYLTQNAFRAWLPDHFDWYIVAAIPLSFLAAAAVGA
VLERGVIRFLYGRPLETLLATWGISLVLMQGVRSLFGAQNVPVENPAWLSGGVQIMSNLVLPWNRIAIVVFAAIVLGGIA
FLIAKTRLGLFVRSVTQNRPMASCMGVNTARVDTLAFALGAGVAGLAGCAVSQVGNVGPDLGQGYIVDSFMVVVLGGVGQ
LAGTVYAALGLGVINKFLEGWAGAVLAKIIVLVFIVVFIQKRPQGLFALKGREA
>Mature_534_residues
MNALRAFLLSLCAAGLLGLAAAPARAMTPAQALAISAGDTDTRVAALQQAIAALDPRVPVLVQALLDGEVKVDDQRAYIV
AADGSAVDAATGQPATPPETAEDAVNNNRMRGELQAALAALQLITGDLTQRRAAIGSLREAMDEAKLPLIEQALAKEADP
ALKAELETLRAKGLIASTDPAKRLQAAQALASSTTPATRSLLLERLKPAAEGGEADPAVRAALQASLDGVNSMLAWGERA
GVVFTGISLGSILLLAALGLAITYGLMGVINMAHGELIMIGAYATYLTQNAFRAWLPDHFDWYIVAAIPLSFLAAAAVGA
VLERGVIRFLYGRPLETLLATWGISLVLMQGVRSLFGAQNVPVENPAWLSGGVQIMSNLVLPWNRIAIVVFAAIVLGGIA
FLIAKTRLGLFVRSVTQNRPMASCMGVNTARVDTLAFALGAGVAGLAGCAVSQVGNVGPDLGQGYIVDSFMVVVLGGVGQ
LAGTVYAALGLGVINKFLEGWAGAVLAKIIVLVFIVVFIQKRPQGLFALKGREA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=295, Percent_Identity=28.4745762711864, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 55407; Mature: 55407

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALRAFLLSLCAAGLLGLAAAPARAMTPAQALAISAGDTDTRVAALQQAIAALDPRVPV
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCHHH
LVQALLDGEVKVDDQRAYIVAADGSAVDAATGQPATPPETAEDAVNNNRMRGELQAALAA
HHHHHHCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHH
LQLITGDLTQRRAAIGSLREAMDEAKLPLIEQALAKEADPALKAELETLRAKGLIASTDP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCH
AKRLQAAQALASSTTPATRSLLLERLKPAAEGGEADPAVRAALQASLDGVNSMLAWGERA
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GVVFTGISLGSILLLAALGLAITYGLMGVINMAHGELIMIGAYATYLTQNAFRAWLPDHF
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCC
DWYIVAAIPLSFLAAAAVGAVLERGVIRFLYGRPLETLLATWGISLVLMQGVRSLFGAQN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VPVENPAWLSGGVQIMSNLVLPWNRIAIVVFAAIVLGGIAFLIAKTRLGLFVRSVTQNRP
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MASCMGVNTARVDTLAFALGAGVAGLAGCAVSQVGNVGPDLGQGYIVDSFMVVVLGGVGQ
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHH
LAGTVYAALGLGVINKFLEGWAGAVLAKIIVLVFIVVFIQKRPQGLFALKGREA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MNALRAFLLSLCAAGLLGLAAAPARAMTPAQALAISAGDTDTRVAALQQAIAALDPRVPV
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCHHH
LVQALLDGEVKVDDQRAYIVAADGSAVDAATGQPATPPETAEDAVNNNRMRGELQAALAA
HHHHHHCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHH
LQLITGDLTQRRAAIGSLREAMDEAKLPLIEQALAKEADPALKAELETLRAKGLIASTDP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCH
AKRLQAAQALASSTTPATRSLLLERLKPAAEGGEADPAVRAALQASLDGVNSMLAWGERA
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GVVFTGISLGSILLLAALGLAITYGLMGVINMAHGELIMIGAYATYLTQNAFRAWLPDHF
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCC
DWYIVAAIPLSFLAAAAVGAVLERGVIRFLYGRPLETLLATWGISLVLMQGVRSLFGAQN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VPVENPAWLSGGVQIMSNLVLPWNRIAIVVFAAIVLGGIAFLIAKTRLGLFVRSVTQNRP
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MASCMGVNTARVDTLAFALGAGVAGLAGCAVSQVGNVGPDLGQGYIVDSFMVVVLGGVGQ
HHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHH
LAGTVYAALGLGVINKFLEGWAGAVLAKIIVLVFIVVFIQKRPQGLFALKGREA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]