Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 124265890

GI number: 124265890

Start: 726513

End: 727790

Strand: Direct

Name: amiC [H]

Synonym: Mpe_A0697

Alternate gene names: 124265890

Gene position: 726513-727790 (Clockwise)

Preceding gene: 124265889

Following gene: 124265891

Centisome position: 17.96

GC content: 63.62

Gene sequence:

>1278_bases
ATGTCCCACACCACCCGCCGCGCCTTTGCGGCCTCGCTTTCCGCCGTGGCGCTGGCCGCTGCCGGCGCCTTCGTGCCCAG
CGCCTTCGCGGCCGACACCATCAAGGTCGGCGTGCTGCACTCGCTGTCGGGCACCATGGCCATCTCGGAAACCGTGCTCA
AGGACACGGTGCTCATGACCATCGACGAGATCAACGCCAAGGGCGGCGTCATGGGCAAGAAGCTCGAGCCGGTGGTGGTG
GACCCGGCCTCCAACTGGCCGCTGTTCGCCGAGAAGGCCAAGCAGCTGATCAGCCAGGACAAGGTGGCCGTAGTGTTCGG
CTGCTGGACGTCGGTGAGCCGCAAGTCGGTGCTGCCGGTGTTCGAGGAAACCAACTCGCTGCTGTTCTACCCGGTGCAGT
ACGAGGGTGAGGAACTGTCCAAGAACGTGTTCTACACCGGTGCCGCGCCCAACCAGCAGGCCATCCCCGCGGTGGAATAC
CTGATGAGCAAGGACGGTGGCTCGGCCAAGCGCTTCGTGCTGCTGGGCACCGATTACGTCTACCCGCGCACCACCAACAA
GATCCTGCGTGCCTTCCTCAAGAGCAAGGGCGTCGCCGAGGCCGACATCATGGAGGAGTACACCCCCTTCGGCCACTCCG
ATTACCAGACCATCATCGCCAAGATCAAGAAGTTCTCGTCGGAAGGCAAGAAGACCGCGGTGGTGTCCACCATCAACGGT
GACTCCAACGTGCCCTTCTACAAGGAACTGGGCAACCAGGGCCTGAAGGCGACCGACGTGCCGGTGGTGGCGTTCTCGGT
GGGTGAGGAAGAGCTCCGCGGTGTCGACACCAAGCCGCTGGTGGGCCACCTGGCGGCGTGGAACTACTTCATGTCGATCA
AGAACCCGACCAACGCCGCGTTCATCAAGCAGTGGAGCGACTACGCCAAGGCCAAGGGCATCCCGGGCCACAAGGACAAG
CCGCTGACCAACGACCCGATGGAGGCCACCTACATCGGCATCCACATGTGGGCGCAGGCGGTCGAGAAGGCCAAGTCCAC
CGACACCGACAAGGTCATCGCCGCCATGGCGGGCCAGACCTTCAAGGCGCCGGGCGGCTTCACCAGCACCATGGACAAGG
AAAACCACCACCTGCACAAGCCGGTGTTCATCGGCGAGGTCAAGGCCGACGGCCAGTTCAACGTGGTCTGGAAGACGCCG
GGCCCGGTGGTCGCCGATCCGTGGAGCGACTACATCGCCGAGAACAAGGGCAAGAAGAACGTGCCGGAGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGGTGTCTCTACGCCACCTTGCGTATTCCGCCGCCCGGGGGTTCTGCCTAACCTGCAGCGGTCCGTGAACCCAACCGATC
CCCCGAGGAGGAAACACCCC

Downstream 100 bases:

>100_bases
GCAACGGATAGCCGGCTCTCGATGTTGGCGCGCGCCGGCCGGGCCGGCGGCGATCGGACTGTCCACCAGGTCGCCGCCGG
TCCTGCCGGCGCGCAGCCTT

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSHTTRRAFAASLSAVALAAAGAFVPSAFAADTIKVGVLHSLSGTMAISETVLKDTVLMTIDEINAKGGVMGKKLEPVVV
DPASNWPLFAEKAKQLISQDKVAVVFGCWTSVSRKSVLPVFEETNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEY
LMSKDGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVAEADIMEEYTPFGHSDYQTIIAKIKKFSSEGKKTAVVSTING
DSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSIKNPTNAAFIKQWSDYAKAKGIPGHKDK
PLTNDPMEATYIGIHMWAQAVEKAKSTDTDKVIAAMAGQTFKAPGGFTSTMDKENHHLHKPVFIGEVKADGQFNVVWKTP
GPVVADPWSDYIAENKGKKNVPEKK

Sequences:

>Translated_425_residues
MSHTTRRAFAASLSAVALAAAGAFVPSAFAADTIKVGVLHSLSGTMAISETVLKDTVLMTIDEINAKGGVMGKKLEPVVV
DPASNWPLFAEKAKQLISQDKVAVVFGCWTSVSRKSVLPVFEETNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEY
LMSKDGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVAEADIMEEYTPFGHSDYQTIIAKIKKFSSEGKKTAVVSTING
DSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSIKNPTNAAFIKQWSDYAKAKGIPGHKDK
PLTNDPMEATYIGIHMWAQAVEKAKSTDTDKVIAAMAGQTFKAPGGFTSTMDKENHHLHKPVFIGEVKADGQFNVVWKTP
GPVVADPWSDYIAENKGKKNVPEKK
>Mature_424_residues
SHTTRRAFAASLSAVALAAAGAFVPSAFAADTIKVGVLHSLSGTMAISETVLKDTVLMTIDEINAKGGVMGKKLEPVVVD
PASNWPLFAEKAKQLISQDKVAVVFGCWTSVSRKSVLPVFEETNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYL
MSKDGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVAEADIMEEYTPFGHSDYQTIIAKIKKFSSEGKKTAVVSTINGD
SNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSIKNPTNAAFIKQWSDYAKAKGIPGHKDKP
LTNDPMEATYIGIHMWAQAVEKAKSTDTDKVIAAMAGQTFKAPGGFTSTMDKENHHLHKPVFIGEVKADGQFNVVWKTPG
PVVADPWSDYIAENKGKKNVPEKK

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46178; Mature: 46047

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTTRRAFAASLSAVALAAAGAFVPSAFAADTIKVGVLHSLSGTMAISETVLKDTVLMT
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEHHHCCCCHHHHHHHHHHHHEEE
IDEINAKGGVMGKKLEPVVVDPASNWPLFAEKAKQLISQDKVAVVFGCWTSVSRKSVLPV
EECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
FEETNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYLMSKDGGSAKRFVLLGTDYV
EECCCCEEEEEEEECCCHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCEE
YPRTTNKILRAFLKSKGVAEADIMEEYTPFGHSDYQTIIAKIKKFSSEGKKTAVVSTING
CCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECC
DSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSIKNPTNAA
CCCCCHHHHHCCCCCEECCCCEEEEECCHHHHCCCCCCHHHHHHHHHEEEEEECCCCCHH
FIKQWSDYAKAKGIPGHKDKPLTNDPMEATYIGIHMWAQAVEKAKSTDTDKVIAAMAGQT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
FKAPGGFTSTMDKENHHLHKPVFIGEVKADGQFNVVWKTPGPVVADPWSDYIAENKGKKN
EECCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCEEECCHHHHHHCCCCCCC
VPEKK
CCCCC
>Mature Secondary Structure 
SHTTRRAFAASLSAVALAAAGAFVPSAFAADTIKVGVLHSLSGTMAISETVLKDTVLMT
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEHHHCCCCHHHHHHHHHHHHEEE
IDEINAKGGVMGKKLEPVVVDPASNWPLFAEKAKQLISQDKVAVVFGCWTSVSRKSVLPV
EECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
FEETNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYLMSKDGGSAKRFVLLGTDYV
EECCCCEEEEEEEECCCHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCEE
YPRTTNKILRAFLKSKGVAEADIMEEYTPFGHSDYQTIIAKIKKFSSEGKKTAVVSTING
CCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECC
DSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSIKNPTNAA
CCCCCHHHHHCCCCCEECCCCEEEEECCHHHHCCCCCCHHHHHHHHHEEEEEECCCCCHH
FIKQWSDYAKAKGIPGHKDKPLTNDPMEATYIGIHMWAQAVEKAKSTDTDKVIAAMAGQT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
FKAPGGFTSTMDKENHHLHKPVFIGEVKADGQFNVVWKTPGPVVADPWSDYIAENKGKKN
EECCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCEEECCHHHHHHCCCCCCC
VPEKK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]