Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 124265821

GI number: 124265821

Start: 662252

End: 663325

Strand: Reverse

Name: rfbB [H]

Synonym: Mpe_A0628

Alternate gene names: 124265821

Gene position: 663325-662252 (Counterclockwise)

Preceding gene: 124265822

Following gene: 124265820

Centisome position: 16.4

GC content: 66.57

Gene sequence:

>1074_bases
ATGATCCTCGTGACCGGCGGTGCCGGTTTCATCGGCAGCAACTTCGTTCTCGACTGGATCGCATCGACCGACGAGCCGGT
CGTCAACCTCGACGTCCTCTCCTACTCCGGCAACCTGCGCAATCTCGCCTCGTTGCAAGGGAATGCCCGGCATGTCTTCG
TGCGCGGCGACATCCGCGATCGGCCGTTGCTCGACCGGCTGCTGGCCGACCACCGGCCCCGGGCGATCGTTCATCTGGCC
GCAGAAAGCCATGTCGATCGGTCTATCCACGGGCCCGGGGCCTTCTTGCACACCAATGTCGAAGGCTCGTTCGTGCTGCT
CGAAGCCGCGCGCGCGTACTGGTCCGGATTAGCCGCCGGCAGGCGGGAATCGTTCCGCTTCCTCCACGTCAGCACCGACG
AGGTGTTCGGCTCGCTCGAGCCCGGGGCACCGGCCTTCACCGAAACACACGCCTACGATCCGAGCAGCCCCTACGCGGCC
AGCAAGGCCGCAAGCGACCACCTCGCCCGAGCCTGGCATCGCACCTACGATCTGCCGGTGGTGACGTCGAACTGTTCGAA
CAACTACGGGCCCTACCATTTCCCCGAGAAACTGATCCCGCTGACGATCGTCAATGCGCTCGCCGGCCAGACACTGCCGG
TCTACGGCGATGGCCTGCAGATCCGCGATTGGCTGCACGTCGGCGACCACTGCCGGGCGCTCCGTACCGTGCTCGAACGA
GGCCGTGTCGGCGAGACCTACAACATCGGCGGAGGGAGCGAGTTCGCCAACATCGACACCGTGCGGCGCGTGTGCGCGCT
GCTCGACGAACTGCAGCCCGATCCCGCCGGCCCGTACGAACGCCTGATCCGCCTGGTCGCCGACCGTCCCGGTCATGACC
GGCGCTACGCGATCGACACCCGCAAGATCGAACGGGAGCTGGGCTGGCGGCCGACCGAAACCTTCGAGACGGGGCTCCGC
AAGACGGTCCAGTGGTACCTTGCTCATGCCGGCTGGGTGCGCGAGGTGCAAAGCGGCAGCTACCGCGACTGGGTGGCACA
GAACTACGCCACGCGAACCCCAGGCCCCGCATGA

Upstream 100 bases:

>100_bases
AGATCATCGAGGTGCAGTCCGGCAGCTATCTCGGTGAGGACGACATCGTGCGCTTCTCCGACACCTACGGCAGAGCGGAC
GGAGTCGGCACGGACCGCAA

Downstream 100 bases:

>100_bases
GGATCCTGCTGCTCGGCAAGAACGGCCAGGTGGGCTGGGAGCTCCAGCGCGCGCTCGCCCCCCTCGGCGAACTGCTCGCG
CTGGCGCGCGACAGCGCGCC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MILVTGGAGFIGSNFVLDWIASTDEPVVNLDVLSYSGNLRNLASLQGNARHVFVRGDIRDRPLLDRLLADHRPRAIVHLA
AESHVDRSIHGPGAFLHTNVEGSFVLLEAARAYWSGLAAGRRESFRFLHVSTDEVFGSLEPGAPAFTETHAYDPSSPYAA
SKAASDHLARAWHRTYDLPVVTSNCSNNYGPYHFPEKLIPLTIVNALAGQTLPVYGDGLQIRDWLHVGDHCRALRTVLER
GRVGETYNIGGGSEFANIDTVRRVCALLDELQPDPAGPYERLIRLVADRPGHDRRYAIDTRKIERELGWRPTETFETGLR
KTVQWYLAHAGWVREVQSGSYRDWVAQNYATRTPGPA

Sequences:

>Translated_357_residues
MILVTGGAGFIGSNFVLDWIASTDEPVVNLDVLSYSGNLRNLASLQGNARHVFVRGDIRDRPLLDRLLADHRPRAIVHLA
AESHVDRSIHGPGAFLHTNVEGSFVLLEAARAYWSGLAAGRRESFRFLHVSTDEVFGSLEPGAPAFTETHAYDPSSPYAA
SKAASDHLARAWHRTYDLPVVTSNCSNNYGPYHFPEKLIPLTIVNALAGQTLPVYGDGLQIRDWLHVGDHCRALRTVLER
GRVGETYNIGGGSEFANIDTVRRVCALLDELQPDPAGPYERLIRLVADRPGHDRRYAIDTRKIERELGWRPTETFETGLR
KTVQWYLAHAGWVREVQSGSYRDWVAQNYATRTPGPA
>Mature_357_residues
MILVTGGAGFIGSNFVLDWIASTDEPVVNLDVLSYSGNLRNLASLQGNARHVFVRGDIRDRPLLDRLLADHRPRAIVHLA
AESHVDRSIHGPGAFLHTNVEGSFVLLEAARAYWSGLAAGRRESFRFLHVSTDEVFGSLEPGAPAFTETHAYDPSSPYAA
SKAASDHLARAWHRTYDLPVVTSNCSNNYGPYHFPEKLIPLTIVNALAGQTLPVYGDGLQIRDWLHVGDHCRALRTVLER
GRVGETYNIGGGSEFANIDTVRRVCALLDELQPDPAGPYERLIRLVADRPGHDRRYAIDTRKIERELGWRPTETFETGLR
KTVQWYLAHAGWVREVQSGSYRDWVAQNYATRTPGPA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=36.1702127659575, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=25.7575757575758, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=26.1764705882353, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=26.1764705882353, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=26.1764705882353, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=59.8885793871866, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=60.7558139534884, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=25.7062146892655, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=300, Percent_Identity=26.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=34.1389728096677, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=32.5443786982249, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=24.6246246246246, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=347, Percent_Identity=24.207492795389, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=276, Percent_Identity=25.3623188405797, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=276, Percent_Identity=25.3623188405797, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=276, Percent_Identity=25.3623188405797, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=216, Percent_Identity=26.3888888888889, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=288, Percent_Identity=25.3472222222222, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=356, Percent_Identity=24.438202247191, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19923002, Length=360, Percent_Identity=27.2222222222222, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39700; Mature: 39700

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGSNFVLDWIASTDEPVVNLDVLSYSGNLRNLASLQGNARHVFVRGDIRD
CEEEECCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCHHHHHCCCCCEEEEEECCCCC
RPLLDRLLADHRPRAIVHLAAESHVDRSIHGPGAFLHTNVEGSFVLLEAARAYWSGLAAG
CHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHCC
RRESFRFLHVSTDEVFGSLEPGAPAFTETHAYDPSSPYAASKAASDHLARAWHRTYDLPV
CCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
VTSNCSNNYGPYHFPEKLIPLTIVNALAGQTLPVYGDGLQIRDWLHVGDHCRALRTVLER
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHC
GRVGETYNIGGGSEFANIDTVRRVCALLDELQPDPAGPYERLIRLVADRPGHDRRYAIDT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEHH
RKIERELGWRPTETFETGLRKTVQWYLAHAGWVREVQSGSYRDWVAQNYATRTPGPA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MILVTGGAGFIGSNFVLDWIASTDEPVVNLDVLSYSGNLRNLASLQGNARHVFVRGDIRD
CEEEECCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCHHHHHCCCCCEEEEEECCCCC
RPLLDRLLADHRPRAIVHLAAESHVDRSIHGPGAFLHTNVEGSFVLLEAARAYWSGLAAG
CHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHCC
RRESFRFLHVSTDEVFGSLEPGAPAFTETHAYDPSSPYAASKAASDHLARAWHRTYDLPV
CCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
VTSNCSNNYGPYHFPEKLIPLTIVNALAGQTLPVYGDGLQIRDWLHVGDHCRALRTVLER
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHC
GRVGETYNIGGGSEFANIDTVRRVCALLDELQPDPAGPYERLIRLVADRPGHDRRYAIDT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEHH
RKIERELGWRPTETFETGLRKTVQWYLAHAGWVREVQSGSYRDWVAQNYATRTPGPA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]