Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 124265675

GI number: 124265675

Start: 523727

End: 524281

Strand: Reverse

Name: ruvC

Synonym: Mpe_A0482

Alternate gene names: 124265675

Gene position: 524281-523727 (Counterclockwise)

Preceding gene: 124265676

Following gene: 124265672

Centisome position: 12.96

GC content: 71.35

Gene sequence:

>555_bases
ATGGCGACACGGATCCTCGGCATCGATCCAGGGCTGCGCACCACCGGATTCGGCGTGGTCGAGTGCGAGGGGTCGCGGCT
GCACTACATCGCCAGCGGCACGGTGCGCACCGACGCGCTGCCGAACGGCGACCTGCCAGGGCGGCTGAAGATCATCTTCG
AGGGTGTGCGCGAGGTCGCGGCGCGCTACCAGCCGGACTGCGCCGCGGTCGAGATCGTGTTCGTCAACGTCAATCCGCAA
TCGACGCTGTTGCTCGGTCAGGCCCGGGGCGCGGCACTGGCGGCGCTGGTGTCGGGCGATCTGGCCGTGGCCGAGTACAC
CGCGTTGCAGATGAAGAAGGCGATCGTCGGCCACGGCCACGCCCGCAAGGAGCAGGTGCAGGCCATGGTGGCGCGGCTGC
TGTCGCTGCCCGGTGAGCCGAGCCGGGACGCAGCCGATGCGCTCGGCCTCGCGGTCTGCCATGCGCACGCGGGCGCGTCG
CAGGCGGCGATCGCCGCCGTCAGCCCGCTGGCGCGGCGCGCCCATGCGCAATACCGCAAGGGCCGCAGCTATTGA

Upstream 100 bases:

>100_bases
TCACCGGTGCGGTGGGCCTGCTCGACCGCGTGGCGCCGCTGTGCTTCGCGGCGCCGGTGTTCTTCCATTCCGTGCGCTGG
TACTTCAACCTGAGCTTCTG

Downstream 100 bases:

>100_bases
CCGGCGCCGAGGCCGGTCGGCGGCGATCAGCCACGCCAGACGACGTTGTGGCGCCGGAAGAGATCGCGCAGGCCTGTGCC
GTTGTTGAGGTCGATCAGGC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MATRILGIDPGLRTTGFGVVECEGSRLHYIASGTVRTDALPNGDLPGRLKIIFEGVREVAARYQPDCAAVEIVFVNVNPQ
STLLLGQARGAALAALVSGDLAVAEYTALQMKKAIVGHGHARKEQVQAMVARLLSLPGEPSRDAADALGLAVCHAHAGAS
QAAIAAVSPLARRAHAQYRKGRSY

Sequences:

>Translated_184_residues
MATRILGIDPGLRTTGFGVVECEGSRLHYIASGTVRTDALPNGDLPGRLKIIFEGVREVAARYQPDCAAVEIVFVNVNPQ
STLLLGQARGAALAALVSGDLAVAEYTALQMKKAIVGHGHARKEQVQAMVARLLSLPGEPSRDAADALGLAVCHAHAGAS
QAAIAAVSPLARRAHAQYRKGRSY
>Mature_183_residues
ATRILGIDPGLRTTGFGVVECEGSRLHYIASGTVRTDALPNGDLPGRLKIIFEGVREVAARYQPDCAAVEIVFVNVNPQS
TLLLGQARGAALAALVSGDLAVAEYTALQMKKAIVGHGHARKEQVQAMVARLLSLPGEPSRDAADALGLAVCHAHAGASQ
AAIAAVSPLARRAHAQYRKGRSY

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=152, Percent_Identity=51.9736842105263, Blast_Score=135, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_METPP (A2SD05)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019679.1
- ProteinModelPortal:   A2SD05
- SMR:   A2SD05
- STRING:   A2SD05
- GeneID:   4787067
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0482
- NMPDR:   fig|279263.3.peg.2748
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   A2SD05
- ProtClustDB:   PRK00039
- BioCyc:   MPET420662:MPE_A0482-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19273; Mature: 19142

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRILGIDPGLRTTGFGVVECEGSRLHYIASGTVRTDALPNGDLPGRLKIIFEGVREVA
CCCEEEECCCCCCCCCCEEEEECCCEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHH
ARYQPDCAAVEIVFVNVNPQSTLLLGQARGAALAALVSGDLAVAEYTALQMKKAIVGHGH
HHCCCCCEEEEEEEEEECCCCEEEEECCCCHHEEHHHCCCHHHHHHHHHHHHHHHHCCCC
ARKEQVQAMVARLLSLPGEPSRDAADALGLAVCHAHAGASQAAIAAVSPLARRAHAQYRK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GRSY
CCCC
>Mature Secondary Structure 
ATRILGIDPGLRTTGFGVVECEGSRLHYIASGTVRTDALPNGDLPGRLKIIFEGVREVA
CCEEEECCCCCCCCCCEEEEECCCEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHH
ARYQPDCAAVEIVFVNVNPQSTLLLGQARGAALAALVSGDLAVAEYTALQMKKAIVGHGH
HHCCCCCEEEEEEEEEECCCCEEEEECCCCHHEEHHHCCCHHHHHHHHHHHHHHHHCCCC
ARKEQVQAMVARLLSLPGEPSRDAADALGLAVCHAHAGASQAAIAAVSPLARRAHAQYRK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GRSY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA