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The map label for this gene is rlmL [H]

Identifier: 124265340

GI number: 124265340

Start: 153382

End: 155721

Strand: Reverse

Name: rlmL [H]

Synonym: Mpe_A0147

Alternate gene names: 124265340

Gene position: 155721-153382 (Counterclockwise)

Preceding gene: 124265341

Following gene: 124265337

Centisome position: 3.85

GC content: 72.26

Gene sequence:

>2340_bases
GTGTCGACCGAGGCGCGAGCGCAACCCTCGAACCCCTCTGCACGCATGAACGTCGCCCTTCCGCCCGACGCCCCGTCGTC
CTCCTTGTTCCTCCCCTGCGCGGCCGGCGTCGAGGCGCTGCTCGCCGACGAGGTGGCCCGGCTGCTGCCGGGCGCGGCGG
CGCAGACGCAGCGCGGCGGCGTGATGGTGCGCGGCGGCCCGCGCGAGGCGATGGCACTCAACCTGGGCAGCCGGCTCGCC
CAGCGCGTGCTGTGGCAGGTGGCCGATGCGCCCTACCGCAGCGAGGACGACCTGTACGCGTTGGCCCGCAGCCCGCGCTG
GCCCGACTGGATCACGCCGACGCAGACCCTGAAGGTGGACGTGACCGCGCAGCGCGCGCCGCTCAAGAGCCTGAACTTCG
CCGCGCTGCGCATCAAGGACGGTGTCTGCGACGCGATGCGCGACGCGCTCGGCGCGCGCCCGAGCGTCGACACCCGCCGC
CCCGACCTGCCGCTGCTGCTGCACCTAGCGCCCGAGCACGCCACGCTGTATGTCGACACCTCGGGCGAGGCGCTGTTCAA
GCGCGGCTGGCGCGAGGACCAGGGCGAGGCGCCACTGAAGGAGACGCTGGCTGCCGCGATGCTGGCCGCCGCCGGCTGGC
AGGGCCGCGAGGCCGACGGCCCGCTGCTCGACCCGGTGTGCGGCTCCGGCACGATCGCGATCGAGGCGGCGCAGATCGCC
TGCGGCATTGCCCCCGGCTCGCTGCGGCGCTTCGCGTTCGAGCGCCTCGGGCCGTTCCGCGACGCCGGCACCCAGCGTGC
CTGGCGCGAGCTGCGCGAGGCCGCGCGGGCGGCCGTGCATGCCAGCGCGGTGCCCATCTTCGCCGGCGACGTGGCCTTCC
GCATGACCGATTTCGCGGCGCGCAACGCGGCACGCGCCGGCGTCGCTGCCGCGATCGAGCTGAAGACGGTCGACGCGCTG
CAGCGCGCCGCCCCCGCCGAACGCGGCACGCTGATGCTCAACCCGCCCTATGGCGAGCGCATCGACCCCAAGGGCTCACG
CGGCGACGGGGCCGGACGGCGCCTCGGCCCACGAGTTCTTCACCCGCCTCGCCACCCACTGGAAGCGCGCCTACCCGGGC
TGGACCGCCTGGGTGCTCAGCCCCGACATGAAGCTGCCGCAGGCGATGCGCCTGAAGGAGAGCCGGCGCGTGCCGATGTG
GAACGGCCCGATCGAGTGCCGGCTGTTCCGCTTCGATCTGGTGGCCGGTTCGATGCGCGAACCGGGCTGAGGGCGCGCCG
GCTGCCCTCAGCGCGGCACTTCGATCTCGACCGGCGCCGACGTGGCCATCACCTGCTGGTCGGCGTTCTGCAGCAGTTCG
ACCCTGAACTTGTAGGTGCCCGGCGGCGGCGCGAGCCAGACCTCGGTCAGGCCCTTGACGAGGTCGATGCGCTCGGTGGG
CGCACCGGCCCGCTCGGCGACCAGCCGGAAGTGGCCCACGCCCTTGTCCTGGATGTCCACGTGGCCCACGTTCAGGCCCG
AGGCGTGCAGTCGCACCCGGAACGGCGTGCGCAGCGGCTCGCCGCTGCGCGGCTCGAGGATCGCGACGCCGGGCTGGATC
AGCGTCTTCGGGTCGACGTCGTCGTTCTTCTTCGTCACCGTGATCTTCATCGGCTTGCTGTAGACGAAGTAGGGGATGTG
CTTGTGGTCCGCCAGCAGCAGCCGCAGCGTGTAGGTGCCGGGCACGAAGGTCAGCACCGTCTCCATCTGGCCCTTGCCGA
AGTGGATGTACTGGTCGTTGAAGGGCAGCGGCTGGGTGAAGTCGAGCGGCAGGTCGCGGTTGACCAGCAGGTGGTGGTGA
CCGGCGCCGTCGACCGGCTTGACGATCGGCGCCAGCCCGTAGCGCGACAGGCCGAACTTCAGCACGAAGGGCGTCTCGAT
GCTGTCGCCGTCGCGCAGGTTGGTGAAGTAGGTCTCGGGCTGCTGGGTGCGCAGCGGCGAGCTGAGCCACGGGTGGTCGG
CCGCGAGGGCCGGTGCGGCGAGCAGGGCGGCCGCGAACCAGGCGAAGCAAGAGCGCATCGTGGAGGTCATTCGGTCGGAG
GTCGTGAAGGTGTCGGTCAGCGCAGCACCGGAGTGTCCGGCAATTCGTTCGGATTGCCATCCCCTCTCGCCGCCGGGAAA
TGCTGGCGCAGCACGGCGTCCACGGCGTCGATCGCGGCGTGCAGCCCGTCCTCGAAGCGCCGCTCGCGGAACGCCGCGCC
CATGCCGTCGACGATGGCCTGCCATCCCGCAGCGTCCACGTGCCGCGTGAGGCCGCGGTCGGCCACGATCTCGATGCGGT
GGTCGGCCAGCAGCAGGTAG

Upstream 100 bases:

>100_bases
CGCGAGGCGCTGAAGATCGCGCCGGCCAACCTGCGGCGCACGATGATGTAACGACGAGGTCTCGGGCCCGAACCGGCAAC
AAGACCCGGCGCCCCGCCCG

Downstream 100 bases:

>100_bases
ATCAGCACGCCGTTGTTGTGCTCGGTGTCCCACACGCGCAGCTTGCCGAACAGCGACAGCGCGCGATCGTGAGCGTCGAC
GTTGCGCCACAGGTAGCCCA

Product: N6-adenine-specific DNA methylase-like protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 779; Mature: 778

Protein sequence:

>779_residues
MSTEARAQPSNPSARMNVALPPDAPSSSLFLPCAAGVEALLADEVARLLPGAAAQTQRGGVMVRGGPREAMALNLGSRLA
QRVLWQVADAPYRSEDDLYALARSPRWPDWITPTQTLKVDVTAQRAPLKSLNFAALRIKDGVCDAMRDALGARPSVDTRR
PDLPLLLHLAPEHATLYVDTSGEALFKRGWREDQGEAPLKETLAAAMLAAAGWQGREADGPLLDPVCGSGTIAIEAAQIA
CGIAPGSLRRFAFERLGPFRDAGTQRAWRELREAARAAVHASAVPIFAGDVAFRMTDFAARNAARAGVAAAIELKTVDAL
QRAAPAERGTLMLNPPYGERIDPKGSRGDGAGRRLGPRVLHPPRHPLEARLPGLDRLGAQPRHEAAAGDAPEGEPARADV
ERPDRVPAVPLRSGGRFDARTGLRARRLPSARHFDLDRRRRGHHLLVGVLQQFDPELVGARRRREPDLGQALDEVDALGG
RTGPLGDQPEVAHALVLDVHVAHVQARGVQSHPERRAQRLAAARLEDRDAGLDQRLRVDVVVLLRHRDLHRLAVDEVGDV
LVVRQQQPQRVGAGHEGQHRLHLALAEVDVLVVEGQRLGEVERQVAVDQQVVVTGAVDRLDDRRQPVARQAELQHEGRLD
AVAVAQVGEVGLGLLGAQRRAEPRVVGREGRCGEQGGREPGEARAHRGGHSVGGREGVGQRSTGVSGNSFGLPSPLAAGK
CWRSTASTASIAACSPSSKRRSRNAAPMPSTMACHPAASTCRVRPRSATISMRWSASSR

Sequences:

>Translated_779_residues
MSTEARAQPSNPSARMNVALPPDAPSSSLFLPCAAGVEALLADEVARLLPGAAAQTQRGGVMVRGGPREAMALNLGSRLA
QRVLWQVADAPYRSEDDLYALARSPRWPDWITPTQTLKVDVTAQRAPLKSLNFAALRIKDGVCDAMRDALGARPSVDTRR
PDLPLLLHLAPEHATLYVDTSGEALFKRGWREDQGEAPLKETLAAAMLAAAGWQGREADGPLLDPVCGSGTIAIEAAQIA
CGIAPGSLRRFAFERLGPFRDAGTQRAWRELREAARAAVHASAVPIFAGDVAFRMTDFAARNAARAGVAAAIELKTVDAL
QRAAPAERGTLMLNPPYGERIDPKGSRGDGAGRRLGPRVLHPPRHPLEARLPGLDRLGAQPRHEAAAGDAPEGEPARADV
ERPDRVPAVPLRSGGRFDARTGLRARRLPSARHFDLDRRRRGHHLLVGVLQQFDPELVGARRRREPDLGQALDEVDALGG
RTGPLGDQPEVAHALVLDVHVAHVQARGVQSHPERRAQRLAAARLEDRDAGLDQRLRVDVVVLLRHRDLHRLAVDEVGDV
LVVRQQQPQRVGAGHEGQHRLHLALAEVDVLVVEGQRLGEVERQVAVDQQVVVTGAVDRLDDRRQPVARQAELQHEGRLD
AVAVAQVGEVGLGLLGAQRRAEPRVVGREGRCGEQGGREPGEARAHRGGHSVGGREGVGQRSTGVSGNSFGLPSPLAAGK
CWRSTASTASIAACSPSSKRRSRNAAPMPSTMACHPAASTCRVRPRSATISMRWSASSR
>Mature_778_residues
STEARAQPSNPSARMNVALPPDAPSSSLFLPCAAGVEALLADEVARLLPGAAAQTQRGGVMVRGGPREAMALNLGSRLAQ
RVLWQVADAPYRSEDDLYALARSPRWPDWITPTQTLKVDVTAQRAPLKSLNFAALRIKDGVCDAMRDALGARPSVDTRRP
DLPLLLHLAPEHATLYVDTSGEALFKRGWREDQGEAPLKETLAAAMLAAAGWQGREADGPLLDPVCGSGTIAIEAAQIAC
GIAPGSLRRFAFERLGPFRDAGTQRAWRELREAARAAVHASAVPIFAGDVAFRMTDFAARNAARAGVAAAIELKTVDALQ
RAAPAERGTLMLNPPYGERIDPKGSRGDGAGRRLGPRVLHPPRHPLEARLPGLDRLGAQPRHEAAAGDAPEGEPARADVE
RPDRVPAVPLRSGGRFDARTGLRARRLPSARHFDLDRRRRGHHLLVGVLQQFDPELVGARRRREPDLGQALDEVDALGGR
TGPLGDQPEVAHALVLDVHVAHVQARGVQSHPERRAQRLAAARLEDRDAGLDQRLRVDVVVLLRHRDLHRLAVDEVGDVL
VVRQQQPQRVGAGHEGQHRLHLALAEVDVLVVEGQRLGEVERQVAVDQQVVVTGAVDRLDDRRQPVARQAELQHEGRLDA
VAVAQVGEVGLGLLGAQRRAEPRVVGREGRCGEQGGREPGEARAHRGGHSVGGREGVGQRSTGVSGNSFGLPSPLAAGKC
WRSTASTASIAACSPSSKRRSRNAAPMPSTMACHPAASTCRVRPRSATISMRWSASSR

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=295, Percent_Identity=33.8983050847458, Blast_Score=156, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 83594; Mature: 83463

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS51165 THUMP ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEARAQPSNPSARMNVALPPDAPSSSLFLPCAAGVEALLADEVARLLPGAAAQTQRGG
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VMVRGGPREAMALNLGSRLAQRVLWQVADAPYRSEDDLYALARSPRWPDWITPTQTLKVD
EEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEE
VTAQRAPLKSLNFAALRIKDGVCDAMRDALGARPSVDTRRPDLPLLLHLAPEHATLYVDT
EECCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
SGEALFKRGWREDQGEAPLKETLAAAMLAAAGWQGREADGPLLDPVCGSGTIAIEAAQIA
CCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHE
CGIAPGSLRRFAFERLGPFRDAGTQRAWRELREAARAAVHASAVPIFAGDVAFRMTDFAA
EECCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHH
RNAARAGVAAAIELKTVDALQRAAPAERGTLMLNPPYGERIDPKGSRGDGAGRRLGPRVL
HHHHHCCCEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HPPRHPLEARLPGLDRLGAQPRHEAAAGDAPEGEPARADVERPDRVPAVPLRSGGRFDAR
CCCCCCHHHCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
TGLRARRLPSARHFDLDRRRRGHHLLVGVLQQFDPELVGARRRREPDLGQALDEVDALGG
CCCHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHCCC
RTGPLGDQPEVAHALVLDVHVAHVQARGVQSHPERRAQRLAAARLEDRDAGLDQRLRVDV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHEEEE
VVLLRHRDLHRLAVDEVGDVLVVRQQQPQRVGAGHEGQHRLHLALAEVDVLVVEGQRLGE
EEEECCCCHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCEEEEEEEEEEEEEECCCHHHH
VERQVAVDQQVVVTGAVDRLDDRRQPVARQAELQHEGRLDAVAVAQVGEVGLGLLGAQRR
HHHHHHCCCEEEEEHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
AEPRVVGREGRCGEQGGREPGEARAHRGGHSVGGREGVGQRSTGVSGNSFGLPSPLAAGK
CCCCEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCH
CWRSTASTASIAACSPSSKRRSRNAAPMPSTMACHPAASTCRVRPRSATISMRWSASSR
HHHCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCC
>Mature Secondary Structure 
STEARAQPSNPSARMNVALPPDAPSSSLFLPCAAGVEALLADEVARLLPGAAAQTQRGG
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VMVRGGPREAMALNLGSRLAQRVLWQVADAPYRSEDDLYALARSPRWPDWITPTQTLKVD
EEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEE
VTAQRAPLKSLNFAALRIKDGVCDAMRDALGARPSVDTRRPDLPLLLHLAPEHATLYVDT
EECCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
SGEALFKRGWREDQGEAPLKETLAAAMLAAAGWQGREADGPLLDPVCGSGTIAIEAAQIA
CCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHE
CGIAPGSLRRFAFERLGPFRDAGTQRAWRELREAARAAVHASAVPIFAGDVAFRMTDFAA
EECCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHH
RNAARAGVAAAIELKTVDALQRAAPAERGTLMLNPPYGERIDPKGSRGDGAGRRLGPRVL
HHHHHCCCEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HPPRHPLEARLPGLDRLGAQPRHEAAAGDAPEGEPARADVERPDRVPAVPLRSGGRFDAR
CCCCCCHHHCCCCHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
TGLRARRLPSARHFDLDRRRRGHHLLVGVLQQFDPELVGARRRREPDLGQALDEVDALGG
CCCHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHCCC
RTGPLGDQPEVAHALVLDVHVAHVQARGVQSHPERRAQRLAAARLEDRDAGLDQRLRVDV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHEEEE
VVLLRHRDLHRLAVDEVGDVLVVRQQQPQRVGAGHEGQHRLHLALAEVDVLVVEGQRLGE
EEEECCCCHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCEEEEEEEEEEEEEECCCHHHH
VERQVAVDQQVVVTGAVDRLDDRRQPVARQAELQHEGRLDAVAVAQVGEVGLGLLGAQRR
HHHHHHCCCEEEEEHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
AEPRVVGREGRCGEQGGREPGEARAHRGGHSVGGREGVGQRSTGVSGNSFGLPSPLAAGK
CCCCEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCH
CWRSTASTASIAACSPSSKRRSRNAAPMPSTMACHPAASTCRVRPRSATISMRWSASSR
HHHCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA