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The map label for this gene is rpoN [H]

Identifier: 124265341

GI number: 124265341

Start: 155771

End: 157294

Strand: Reverse

Name: rpoN [H]

Synonym: Mpe_A0148

Alternate gene names: 124265341

Gene position: 157294-155771 (Counterclockwise)

Preceding gene: 124265342

Following gene: 124265340

Centisome position: 3.89

GC content: 68.31

Gene sequence:

>1524_bases
ATGAAGCCTTCCCTGCAGGTCCGTTTTTCGCAGCATCTGGCGCTCACGCCGCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTCGAGCTTCACCAGGAAGTCGAGCAGATGCTGGAGCAGAACCCCTTCCTCGAAGTGGAGGAAGATGCGC
CGACCCCGTTCGACGCGCCGGTGGAGCGCGCCACGGCCACGGAGCGCCAGGCCGATGACGCCTGGGAGGGCTCGGGGTCC
GAGGTGGCCGCCGACCCCGAGCCGGTCGCGGTGGATGCGGCCGAGTTCGGCACCACCGAGCGCGAGGACTGGGAGAACGG
CACCGAGCGCGAGGACTTCGACGGCATCCGCGAGACGCCCGGCAAGGCCGGCAACAACGACAGCGACGAGTTCGACCCCA
TGGAGCGCAGCAGCGCCGGGGTGAGCCTGCAGGACCACCTGCGCGACCAGTTGCGCGGCATGCGCCTGAGCGACGAGGAC
CGCGGCGCGGTGATGGTGCTGATCGAATCGCTCGACGAGGACGGCTACCTGGCCGACCCCCTGGAAGAGATCGCCCAGCG
CCTGGCCGGCGACGAGGACGACATCGCGGTCGAGGAGCTGCTCGACCGCCTGCGCTGCGCGCTGAAGTGGCTGCACAACC
TGGAGCCGCTGGGCGTCGGTGCGCGCGACCTGTCGGAGTGCCTGACGCTGCAGCTGCGGGCCGGACCGCGCTGCGAGGCG
CAGATGATCGCGATCCTGATCTGCAAGTACCACCTCGAGTTGCTGGCGCGGCGCGACGGCAAGAAGCTGATGGCGGCCAC
CGGCGCCGACGAGGAGCTGCTGAAGGCCGCGCAGGCGCTGATCGTGCGCTGCGAGCCCAAGCCCGGCCGGCCCTTCACCA
AGGCCGAGGCCAACATCATCGTGCCCGATGTCATCGTGCAGAAGGCCGGCCGCGGCTGGCGCGTGGTGCTCAACCCCGAC
GTGATGCCCAAGCTGCGCATCAACGACCTCTACGCCCAGGCCATCAAGCAGCAGCGGGGCGCGCGCACCGAATCGGGCGC
GGGCCTGAGCTCGCGGCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCTGCAGCGCTTCGACACCATCCAGCGCGTGT
CGCAGGCCATCGTCGAGCGGCAGAAGGCCTTCTTCAGCCACGGCGCGATCGCGATGAAGCCACTGGTGCTGCGCGAGATC
GCCGACGAGCTGGGTCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGTCCACGCCCTACGGCACCTT
CGAGCTGAAGTATTTCTTCGGCTCCTCGCTCAACACCGAGGCCGGTGGCAATGCGTCGAGCACCGCGGTGCGTGCGCTGA
TCAAGCAGCTGGTCAGTGCCGAGGATGCCAAGAAGCCGCTGTCGGACAGCCAGCTCAGCAGCATGCTGGAAGAGCAGGGC
ATCCAGGTGGCGCGCCGCACGGTGGCGAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCAACCTGCGGCGCACGATGAT
GTAA

Upstream 100 bases:

>100_bases
TCATCAGCGAAGGACGCGTGCTGGCCGAAGGCACCCCCGAGCAGATCGTCGAGAACGCCGACGTCCGCAAGGTCTACCTC
GGCGAGCACTTCAGGATGTG

Downstream 100 bases:

>100_bases
CGACGAGGTCTCGGGCCCGAACCGGCAACAAGACCCGGCGCCCCGCCCGGTGTCGACCGAGGCGCGAGCGCAACCCTCGA
ACCCCTCTGCACGCATGAAC

Product: putative RNA polymerase sigma N (sigma 54) factor transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAPVERATATERQADDAWEGSGS
EVAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETPGKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDED
RGAVMVLIESLDEDGYLADPLEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANIIVPDVIVQKAGRGWRVVLNPD
VMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFMKNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREI
ADELGLHESTISRVTTAKYMSTPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
IQVARRTVAKYREALKIAPANLRRTMM

Sequences:

>Translated_507_residues
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAPVERATATERQADDAWEGSGS
EVAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETPGKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDED
RGAVMVLIESLDEDGYLADPLEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANIIVPDVIVQKAGRGWRVVLNPD
VMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFMKNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREI
ADELGLHESTISRVTTAKYMSTPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
IQVARRTVAKYREALKIAPANLRRTMM
>Mature_507_residues
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAPVERATATERQADDAWEGSGS
EVAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETPGKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDED
RGAVMVLIESLDEDGYLADPLEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANIIVPDVIVQKAGRGWRVVLNPD
VMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFMKNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREI
ADELGLHESTISRVTTAKYMSTPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
IQVARRTVAKYREALKIAPANLRRTMM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=513, Percent_Identity=43.859649122807, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56448; Mature: 56448

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAP
CCCCCEEEEHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
VERATATERQADDAWEGSGSEVAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECHHHCCCCCCHHHCCCCCCHHCCCHHCCC
GKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDEDRGAVMVLIESLDEDGYLADP
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCH
LEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHH
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANII
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCEE
VPDVIVQKAGRGWRVVLNPDVMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFM
CHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
KNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREIADELGLHESTISRVTTAKYM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
STPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IQVARRTVAKYREALKIAPANLRRTMM
HHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAP
CCCCCEEEEHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
VERATATERQADDAWEGSGSEVAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECHHHCCCCCCHHHCCCCCCHHCCCHHCCC
GKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDEDRGAVMVLIESLDEDGYLADP
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCH
LEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHH
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANII
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCEE
VPDVIVQKAGRGWRVVLNPDVMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFM
CHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
KNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREIADELGLHESTISRVTTAKYM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
STPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IQVARRTVAKYREALKIAPANLRRTMM
HHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]