Click here to switch to the map view.

The map label for this gene is clcA [H]

Identifier: 124265303

GI number: 124265303

Start: 112718

End: 114175

Strand: Reverse

Name: clcA [H]

Synonym: Mpe_A0110

Alternate gene names: 124265303

Gene position: 114175-112718 (Counterclockwise)

Preceding gene: 124265320

Following gene: 124265302

Centisome position: 2.82

GC content: 72.7

Gene sequence:

>1458_bases
TTGCATGAGCCGGCCCAGGCATCATCCGTGGGTGCGCGGGCCGCCGCTCGCGCACCGTCGCACCGTCCCGCACCCTTGAA
GAAGCAACCCGACTTCACCCAGGCCGTTCGCCAGGAACTGGTCGACTGGCGCGCCTGGCGCGATCGCGCTGTGGTGCTGG
CGTACGCGGTCGCCGCGGGGCTCGCCGTGGTCGGCTTCACGATGCTGAGCGAGCTGGCGCTCGAGGGCTTCGGTGCCCTG
CGCGCCCTGCACCCGCTGCTGCCGCTGGTCTGGATGCCGGCGCTCACCGCGGCGCTGGTGTGGGTCACGCTGCGCTGGGT
GCCGGGGGCCGCCGGCTCCGGGATTCCGCAGGTGCTGGCGGCGCTGGACACGCGCACCTCGCCGTCGCAGCGTGGGCTGT
TCGTGTCGCTGCGGCTGAGCGTGGCCAAGCTGGTGCTGGCGTCGGGCGCGCTGCTGGCCGGGCTGTCGGCCGGGCGCGAA
GGACCATCGGTGCAGGTGGCGGCCGGAGTGATGCTGCATGCACGCCGCTGGCTGCCGTCGCGATCGAGCGTCACCGACCA
CGGCCTGCTGATCGCCGGCGGCGCCGCCGGCATCGCCGCCGCCTTCAATGCGCCGCTGGCCGGTGTGGTGTTCGCGATCG
AGGAGCTGTCGCGCAAGATCGAGAACCGCAACAGCGGACTCATCATCACCGCCATCGTGCTGGCCGGCCTGGTGGCGGTC
TCGGCCTTCGGCAACTCCACGTACTTCGGCGTCATCCGCGTCGGCCGGCTCGACAGCGGCGTGCTGCTGCCCGGCCTGCT
GGTGGTGCTGTGCGCGGGCCTGCTGGGCGGCCTGTTCTCGCGCCTGCTGATCGTGTCGATGGTCGGTGCGCCCGATCGCT
TCTGCGCGCTGCGGCGGCGCCATCCGGTGCGCTTCGCGGCCGGCTGCGGCTTCGTGGTGGCGCTGATCGGCATCGTCAGC
GGCGGCCTCACCTATGGCAGCGGCTACGACGAGACCAAGGGCCTGCTGGACGGCGAACCCGGCCTGCCGGTGCTGTTCGT
GCTGCTCAAGTTCATCAGCACCTGGCTGTCGACCTGGAGCGGCGTGCCGGGCGGCATCTTCGCGCCCTCGCTGTCGGTCG
GCGCGGGCATTGGCGCCGACATCGCGCAGCTCACCGGCTCGCCGGCCGGCGCGGCGCTGATCGCCATCGGCATGGCCGGC
TTCCTGGCCGCCGTGACGCAGGCGCCGATCACGGCTTTCATCATCGTGATGGAGATGGTCGACGGCCACGCGATGGTGCT
CAGCCTGATGGCTGCGGCGCTGGGGGCCAGCGGCGTCTCGCGGCTGCTGAGCCGGCCGCTGTACGCCGAGCTGGCCGAGC
TGCAGCTGCGACGCCTGCCGGCCGTGCCGTCGACGATGCCGCCGGACTCGGGCACCGCCACCACCGCATTCCAGGACTCC
GCTCCCGGCAGGCCCTGA

Upstream 100 bases:

>100_bases
CGCAGTAAGGAGAAAGAAGGGATTCGGAGGGGTCCGCGGCCCGGGAATCCGGGGCGGCCGATGATTATGCCCGCCACCCC
GATGGCCCTGCGCCCGCTTC

Downstream 100 bases:

>100_bases
CGCCCGCGCCCGGCCGGCACACGCGGGCGCTCCGCTAGCATCCGCCCCGTGACCCCGCCGCGAACCGACCCGACCCTGCC
CGCCACCGACCCGCTGAACG

Product: voltage gated chloride channel family protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MHEPAQASSVGARAAARAPSHRPAPLKKQPDFTQAVRQELVDWRAWRDRAVVLAYAVAAGLAVVGFTMLSELALEGFGAL
RALHPLLPLVWMPALTAALVWVTLRWVPGAAGSGIPQVLAALDTRTSPSQRGLFVSLRLSVAKLVLASGALLAGLSAGRE
GPSVQVAAGVMLHARRWLPSRSSVTDHGLLIAGGAAGIAAAFNAPLAGVVFAIEELSRKIENRNSGLIITAIVLAGLVAV
SAFGNSTYFGVIRVGRLDSGVLLPGLLVVLCAGLLGGLFSRLLIVSMVGAPDRFCALRRRHPVRFAAGCGFVVALIGIVS
GGLTYGSGYDETKGLLDGEPGLPVLFVLLKFISTWLSTWSGVPGGIFAPSLSVGAGIGADIAQLTGSPAGAALIAIGMAG
FLAAVTQAPITAFIIVMEMVDGHAMVLSLMAAALGASGVSRLLSRPLYAELAELQLRRLPAVPSTMPPDSGTATTAFQDS
APGRP

Sequences:

>Translated_485_residues
MHEPAQASSVGARAAARAPSHRPAPLKKQPDFTQAVRQELVDWRAWRDRAVVLAYAVAAGLAVVGFTMLSELALEGFGAL
RALHPLLPLVWMPALTAALVWVTLRWVPGAAGSGIPQVLAALDTRTSPSQRGLFVSLRLSVAKLVLASGALLAGLSAGRE
GPSVQVAAGVMLHARRWLPSRSSVTDHGLLIAGGAAGIAAAFNAPLAGVVFAIEELSRKIENRNSGLIITAIVLAGLVAV
SAFGNSTYFGVIRVGRLDSGVLLPGLLVVLCAGLLGGLFSRLLIVSMVGAPDRFCALRRRHPVRFAAGCGFVVALIGIVS
GGLTYGSGYDETKGLLDGEPGLPVLFVLLKFISTWLSTWSGVPGGIFAPSLSVGAGIGADIAQLTGSPAGAALIAIGMAG
FLAAVTQAPITAFIIVMEMVDGHAMVLSLMAAALGASGVSRLLSRPLYAELAELQLRRLPAVPSTMPPDSGTATTAFQDS
APGRP
>Mature_485_residues
MHEPAQASSVGARAAARAPSHRPAPLKKQPDFTQAVRQELVDWRAWRDRAVVLAYAVAAGLAVVGFTMLSELALEGFGAL
RALHPLLPLVWMPALTAALVWVTLRWVPGAAGSGIPQVLAALDTRTSPSQRGLFVSLRLSVAKLVLASGALLAGLSAGRE
GPSVQVAAGVMLHARRWLPSRSSVTDHGLLIAGGAAGIAAAFNAPLAGVVFAIEELSRKIENRNSGLIITAIVLAGLVAV
SAFGNSTYFGVIRVGRLDSGVLLPGLLVVLCAGLLGGLFSRLLIVSMVGAPDRFCALRRRHPVRFAAGCGFVVALIGIVS
GGLTYGSGYDETKGLLDGEPGLPVLFVLLKFISTWLSTWSGVPGGIFAPSLSVGAGIGADIAQLTGSPAGAALIAIGMAG
FLAAVTQAPITAFIIVMEMVDGHAMVLSLMAAALGASGVSRLLSRPLYAELAELQLRRLPAVPSTMPPDSGTATTAFQDS
APGRP

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=368, Percent_Identity=31.25, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081943, Length=250, Percent_Identity=29.6, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 49850; Mature: 49850

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEPAQASSVGARAAARAPSHRPAPLKKQPDFTQAVRQELVDWRAWRDRAVVLAYAVAAG
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVVGFTMLSELALEGFGALRALHPLLPLVWMPALTAALVWVTLRWVPGAAGSGIPQVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ALDTRTSPSQRGLFVSLRLSVAKLVLASGALLAGLSAGREGPSVQVAAGVMLHARRWLPS
HHHCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCC
RSSVTDHGLLIAGGAAGIAAAFNAPLAGVVFAIEELSRKIENRNSGLIITAIVLAGLVAV
CCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHH
SAFGNSTYFGVIRVGRLDSGVLLPGLLVVLCAGLLGGLFSRLLIVSMVGAPDRFCALRRR
HHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
HPVRFAAGCGFVVALIGIVSGGLTYGSGYDETKGLLDGEPGLPVLFVLLKFISTWLSTWS
CCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GVPGGIFAPSLSVGAGIGADIAQLTGSPAGAALIAIGMAGFLAAVTQAPITAFIIVMEMV
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGHAMVLSLMAAALGASGVSRLLSRPLYAELAELQLRRLPAVPSTMPPDSGTATTAFQDS
CCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCC
APGRP
CCCCC
>Mature Secondary Structure
MHEPAQASSVGARAAARAPSHRPAPLKKQPDFTQAVRQELVDWRAWRDRAVVLAYAVAAG
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVVGFTMLSELALEGFGALRALHPLLPLVWMPALTAALVWVTLRWVPGAAGSGIPQVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ALDTRTSPSQRGLFVSLRLSVAKLVLASGALLAGLSAGREGPSVQVAAGVMLHARRWLPS
HHHCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCC
RSSVTDHGLLIAGGAAGIAAAFNAPLAGVVFAIEELSRKIENRNSGLIITAIVLAGLVAV
CCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHH
SAFGNSTYFGVIRVGRLDSGVLLPGLLVVLCAGLLGGLFSRLLIVSMVGAPDRFCALRRR
HHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
HPVRFAAGCGFVVALIGIVSGGLTYGSGYDETKGLLDGEPGLPVLFVLLKFISTWLSTWS
CCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GVPGGIFAPSLSVGAGIGADIAQLTGSPAGAALIAIGMAGFLAAVTQAPITAFIIVMEMV
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGHAMVLSLMAAALGASGVSRLLSRPLYAELAELQLRRLPAVPSTMPPDSGTATTAFQDS
CCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCC
APGRP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA