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The map label for this gene is cysB [H]

Identifier: 124265320

GI number: 124265320

Start: 129563

End: 130498

Strand: Reverse

Name: cysB [H]

Synonym: Mpe_A0127

Alternate gene names: 124265320

Gene position: 130498-129563 (Counterclockwise)

Preceding gene: 124265321

Following gene: 124265303

Centisome position: 3.23

GC content: 71.26

Gene sequence:

>936_bases
ATGAACTTCCAGCAACTGCGCTCCGTCCGCGAGGCGGTGCGGCTCGGCTTCAACCTCACCGACGTGGCCGAGGCGCTGCA
CACGTCGCAGCCGGGCGTGAGCCGGCAGATCCGCGAGCTCGAAGACGAGCTCGGCGTCGACATCTTCGTGCGCGCCGGCA
AGCGGCTGACCGGGCTCACCGCCCCCGGCGAGGCGGTGCTGCCGATCGTGGAGCGGCTGCTGCACGACGCCGACAACCTC
AAGCGCGCCGGCGCCGACTACGCCCAGCAGGACCACGGCCCGCTGTCCATCGCCGCCACCCACTCGCAGGCGCGCTACGC
GCTGCCGCCAGCGGTGCGCGACTTCCGCGGCAGCCATCCGGGCGTGCAGTTGCAGCTGCACCAGGGCTCGCCGAAGCAGG
TGGCGCAGATGCTGCTCGACGGCGAGGCCGACATCGGCATCGCGACCGAGGCGCTGGCGCAGTACCCCGACCTCGTGGCG
CTGCCCTGCTACCGCTGGACGCACGCGGTGCTGGTGCCGCCCGACCACCCGCTGCTCGACGGCGGCGCCCTCACGCTGAA
GCGGCTCGCCGAATACCCGCTGATCACCTACGACGCCGGCTACACCGGCCGCTCGCACATCGACGAGGCCTTCGAGCGCG
AGGGCCTCACGCCCACGCTGGCGCTGACGGCGATGGACGCCGACGTGATCAAGACCTACGTGGAGTTGGGCCTGGGCGTC
GGCATCGTCGCCTCGATCGCCTACGACGACGAGCGCGATCGCCACCTGCGCGCGCTCGACGCGCGCCACCTGTTCGCCGT
GAACATGACGCGCCTGGCGCTCAAGCGCGGCAGCTACCTGCGGGCCTATGTGTACGACTTCATCGAGAGCTTTGCGCCGT
CGCTGACGCGCAAGGTGGTGCAGCGCGCGCTCGACGGCGGCAAGGTCGAACTCTGA

Upstream 100 bases:

>100_bases
GCGAAAGCAAACAACGAAACGGTCGTTCCCTCGCGCGCGGCCAGCCCGGACACTCGGAAACTTTCGTTTTCGCGAAGCCT
TCGCCTTCGCGGCTGACCCG

Downstream 100 bases:

>100_bases
GCAGTGCGCGGCTCGGTCGGGTGCGACGGCCCGACCGGGACCCGATCAGATCATCGCGGCCGGATCGGCTTCGGACTCCT
GCGCGAAGCGCGTCACGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNFQQLRSVREAVRLGFNLTDVAEALHTSQPGVSRQIRELEDELGVDIFVRAGKRLTGLTAPGEAVLPIVERLLHDADNL
KRAGADYAQQDHGPLSIAATHSQARYALPPAVRDFRGSHPGVQLQLHQGSPKQVAQMLLDGEADIGIATEALAQYPDLVA
LPCYRWTHAVLVPPDHPLLDGGALTLKRLAEYPLITYDAGYTGRSHIDEAFEREGLTPTLALTAMDADVIKTYVELGLGV
GIVASIAYDDERDRHLRALDARHLFAVNMTRLALKRGSYLRAYVYDFIESFAPSLTRKVVQRALDGGKVEL

Sequences:

>Translated_311_residues
MNFQQLRSVREAVRLGFNLTDVAEALHTSQPGVSRQIRELEDELGVDIFVRAGKRLTGLTAPGEAVLPIVERLLHDADNL
KRAGADYAQQDHGPLSIAATHSQARYALPPAVRDFRGSHPGVQLQLHQGSPKQVAQMLLDGEADIGIATEALAQYPDLVA
LPCYRWTHAVLVPPDHPLLDGGALTLKRLAEYPLITYDAGYTGRSHIDEAFEREGLTPTLALTAMDADVIKTYVELGLGV
GIVASIAYDDERDRHLRALDARHLFAVNMTRLALKRGSYLRAYVYDFIESFAPSLTRKVVQRALDGGKVEL
>Mature_311_residues
MNFQQLRSVREAVRLGFNLTDVAEALHTSQPGVSRQIRELEDELGVDIFVRAGKRLTGLTAPGEAVLPIVERLLHDADNL
KRAGADYAQQDHGPLSIAATHSQARYALPPAVRDFRGSHPGVQLQLHQGSPKQVAQMLLDGEADIGIATEALAQYPDLVA
LPCYRWTHAVLVPPDHPLLDGGALTLKRLAEYPLITYDAGYTGRSHIDEAFEREGLTPTLALTAMDADVIKTYVELGLGV
GIVASIAYDDERDRHLRALDARHLFAVNMTRLALKRGSYLRAYVYDFIESFAPSLTRKVVQRALDGGKVEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=304, Percent_Identity=50.6578947368421, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1788296, Length=302, Percent_Identity=52.6490066225166, Blast_Score=321, Evalue=4e-89,
Organism=Escherichia coli, GI157672245, Length=219, Percent_Identity=36.0730593607306, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=31.2757201646091, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788481, Length=251, Percent_Identity=27.0916334661355, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790208, Length=231, Percent_Identity=27.7056277056277, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790399, Length=232, Percent_Identity=23.7068965517241, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=222, Percent_Identity=22.972972972973, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=257, Percent_Identity=28.7937743190661, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081904, Length=212, Percent_Identity=27.8301886792453, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=243, Percent_Identity=25.1028806584362, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34176; Mature: 34176

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50931 HTH_LYSR ; PS50937 HTH_MERR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQQLRSVREAVRLGFNLTDVAEALHTSQPGVSRQIRELEDELGVDIFVRAGKRLTGLT
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCEECCC
APGEAVLPIVERLLHDADNLKRAGADYAQQDHGPLSIAATHSQARYALPPAVRDFRGSHP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCCC
GVQLQLHQGSPKQVAQMLLDGEADIGIATEALAQYPDLVALPCYRWTHAVLVPPDHPLLD
CEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCHHCCEEEECCCCCCCCC
GGALTLKRLAEYPLITYDAGYTGRSHIDEAFEREGLTPTLALTAMDADVIKTYVELGLGV
CHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCH
GIVASIAYDDERDRHLRALDARHLFAVNMTRLALKRGSYLRAYVYDFIESFAPSLTRKVV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QRALDGGKVEL
HHHHCCCCCCC
>Mature Secondary Structure
MNFQQLRSVREAVRLGFNLTDVAEALHTSQPGVSRQIRELEDELGVDIFVRAGKRLTGLT
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCEECCC
APGEAVLPIVERLLHDADNLKRAGADYAQQDHGPLSIAATHSQARYALPPAVRDFRGSHP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCCC
GVQLQLHQGSPKQVAQMLLDGEADIGIATEALAQYPDLVALPCYRWTHAVLVPPDHPLLD
CEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCHHCCEEEECCCCCCCCC
GGALTLKRLAEYPLITYDAGYTGRSHIDEAFEREGLTPTLALTAMDADVIKTYVELGLGV
CHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCH
GIVASIAYDDERDRHLRALDARHLFAVNMTRLALKRGSYLRAYVYDFIESFAPSLTRKVV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QRALDGGKVEL
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]