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The map label for this gene is gatB [H]

Identifier: 124265279

GI number: 124265279

Start: 82273

End: 83721

Strand: Direct

Name: gatB [H]

Synonym: Mpe_A0086

Alternate gene names: 124265279

Gene position: 82273-83721 (Clockwise)

Preceding gene: 124265278

Following gene: 124265282

Centisome position: 2.03

GC content: 68.32

Gene sequence:

>1449_bases
ATGAACGTCGCCCCCGCGCTGATCCGTGGTTACGAAGTCGTCATCGGCCTCGAGACCCACACCCAGCTGTCCACCGCGTC
GAAGATCTTCTCCGGTGCCCCCACCGCCTTCGGCGCGGCGCCGAACACCCAGGCCTGCGCGGTCGACCTGGCTCTGCCCG
GCAGCCTGCCGGTGATGAACCGCGGTGCGGTCGAGCGCGCGATCCGCTTCGGCCTGGCCGTGGGCGGCCAGATCGCGCCG
CTGTCGATCTTCGCGCGCAAGAACTACTTCTATCCCGACCTGCCCAAGGGCTACCAGATCAGCCAGTACGAGCTGCCGGT
GGTGCAGGGCGGCACAGTGGAGTTCTACCTGGCCGACGTGCCGCACCAGGTGCGGCTGACGCGGGCCCATCTCGAGGAAG
ACGCCGGCAAGTCGGTGCACGACGCCTTCGACGGCCTGAGCGGCATCGACCTCAACCGCGCCGGCACGCCGCTGCTGGAG
ATCGTCTCCGAGCCCGACATGCGCTCGTCCAAGGAGGCGGTCGAGTACGCCAAGGCGCTGCACGCGCTGGTGGTGTGGCT
GGGCATCTGCGACGGCAACATGCAGGAAGGCAGCTTCCGCTGCGACGCCAACGTCTCGGTGCGCCGGCCCGGCGCGCCGT
TCGGCACGCGGCGTGAGATCAAGAACCTCAACAGCTTCCGCTTCCTGCAGCAGGCGATCGACTTCGAGATCCAGTGGCAG
ATCGACCAGATCGAGGACGGTCTCGCGATCCGGCAGGCCACCGTGCTGTTCAACCCCGACAGCGGCGAGACGCGAGCGAT
GCGCACCAAGGAAGACGCGCACGACTACCGCTACTTCCCCGACCCTGACCTGCCGCCGCTGGTGATCGCGCCGGAATGGA
TCGCGCGCGTGACCGGCGAAATGCCGGAGCTGCCGCGCGTGATGGCGGCGCGCTTCGAGCGCGAGGACGGCCTAGCCGCC
TACGACGCCTCGATGATGACGCAGAGCCTCGCGGTGGCGCGCTATTACGAGGCCACGAAGGCGGCGGGTGCGCCGGCCAA
GCTGGCGGCGAACTGGCTGATGGGCGAGCTGTCGCGGCGGCTCAATGCCGAGGAACTCGGCATCGATGCGAGCCCGGTGA
GCCCGGCGACGCTCGCGGCGCTGATCGCCCGCATCGCCGACGGCACGATCTCGAACAACGGCGCGAAGCAGGTGTTCGAT
GCGCTGTGGAACGGCGAGACGGACGTCGACGCGGTCATCTCCGCCAAGGGCCTGAAGCAGATGTCCGACAGCGGCGAGCT
CTCCGCCATCGTCGACGCCGTGCTCGCCGCGAATCCGAAGTCGGTGGACGAATACCGCGCCGGCAAGGACAAGGCCTTCA
ACGCGCTGGTCGGCCAGGCGATGAAGGCGAGCAAGGGCAAGGCCAACCCCACGCAACTCAGCGAGCTGCTCAAGAAGAAG
CTCGGTTGA

Upstream 100 bases:

>100_bases
CTGATCGGCAACTACTGGCAGGAGGCGCAGCTGCTGCACGCCGCCCACAGCTTCCAGCAAGCCACCGATTTCCACCTCCG
GACGCCGGAGGGCTACTGAC

Downstream 100 bases:

>100_bases
CGGCGGAGAGCGGCGGCCTCCGGCCGGCGCGGCTCAAGGCTTGGGTGGCGGCGCGGCCTGACCGGCCAGCGCCGACTGCG
GCAGCGGGCCGAGCGATCCC

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNVAPALIRGYEVVIGLETHTQLSTASKIFSGAPTAFGAAPNTQACAVDLALPGSLPVMNRGAVERAIRFGLAVGGQIAP
LSIFARKNYFYPDLPKGYQISQYELPVVQGGTVEFYLADVPHQVRLTRAHLEEDAGKSVHDAFDGLSGIDLNRAGTPLLE
IVSEPDMRSSKEAVEYAKALHALVVWLGICDGNMQEGSFRCDANVSVRRPGAPFGTRREIKNLNSFRFLQQAIDFEIQWQ
IDQIEDGLAIRQATVLFNPDSGETRAMRTKEDAHDYRYFPDPDLPPLVIAPEWIARVTGEMPELPRVMAARFEREDGLAA
YDASMMTQSLAVARYYEATKAAGAPAKLAANWLMGELSRRLNAEELGIDASPVSPATLAALIARIADGTISNNGAKQVFD
ALWNGETDVDAVISAKGLKQMSDSGELSAIVDAVLAANPKSVDEYRAGKDKAFNALVGQAMKASKGKANPTQLSELLKKK
LG

Sequences:

>Translated_482_residues
MNVAPALIRGYEVVIGLETHTQLSTASKIFSGAPTAFGAAPNTQACAVDLALPGSLPVMNRGAVERAIRFGLAVGGQIAP
LSIFARKNYFYPDLPKGYQISQYELPVVQGGTVEFYLADVPHQVRLTRAHLEEDAGKSVHDAFDGLSGIDLNRAGTPLLE
IVSEPDMRSSKEAVEYAKALHALVVWLGICDGNMQEGSFRCDANVSVRRPGAPFGTRREIKNLNSFRFLQQAIDFEIQWQ
IDQIEDGLAIRQATVLFNPDSGETRAMRTKEDAHDYRYFPDPDLPPLVIAPEWIARVTGEMPELPRVMAARFEREDGLAA
YDASMMTQSLAVARYYEATKAAGAPAKLAANWLMGELSRRLNAEELGIDASPVSPATLAALIARIADGTISNNGAKQVFD
ALWNGETDVDAVISAKGLKQMSDSGELSAIVDAVLAANPKSVDEYRAGKDKAFNALVGQAMKASKGKANPTQLSELLKKK
LG
>Mature_482_residues
MNVAPALIRGYEVVIGLETHTQLSTASKIFSGAPTAFGAAPNTQACAVDLALPGSLPVMNRGAVERAIRFGLAVGGQIAP
LSIFARKNYFYPDLPKGYQISQYELPVVQGGTVEFYLADVPHQVRLTRAHLEEDAGKSVHDAFDGLSGIDLNRAGTPLLE
IVSEPDMRSSKEAVEYAKALHALVVWLGICDGNMQEGSFRCDANVSVRRPGAPFGTRREIKNLNSFRFLQQAIDFEIQWQ
IDQIEDGLAIRQATVLFNPDSGETRAMRTKEDAHDYRYFPDPDLPPLVIAPEWIARVTGEMPELPRVMAARFEREDGLAA
YDASMMTQSLAVARYYEATKAAGAPAKLAANWLMGELSRRLNAEELGIDASPVSPATLAALIARIADGTISNNGAKQVFD
ALWNGETDVDAVISAKGLKQMSDSGELSAIVDAVLAANPKSVDEYRAGKDKAFNALVGQAMKASKGKANPTQLSELLKKK
LG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=493, Percent_Identity=38.5395537525355, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=40.2684563758389, Blast_Score=218, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=30.7101727447217, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=34.0122199592668, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52078; Mature: 52078

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00024 HEMOPEXIN ; PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAPALIRGYEVVIGLETHTQLSTASKIFSGAPTAFGAAPNTQACAVDLALPGSLPVMN
CCCCHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
RGAVERAIRFGLAVGGQIAPLSIFARKNYFYPDLPKGYQISQYELPVVQGGTVEFYLADV
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCEEECCEEEEEEECC
PHQVRLTRAHLEEDAGKSVHDAFDGLSGIDLNRAGTPLLEIVSEPDMRSSKEAVEYAKAL
CCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHH
HALVVWLGICDGNMQEGSFRCDANVSVRRPGAPFGTRREIKNLNSFRFLQQAIDFEIQWQ
HHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCHHHHHCCHHHHHHHHHHCEEEEEE
IDQIEDGLAIRQATVLFNPDSGETRAMRTKEDAHDYRYFPDPDLPPLVIAPEWIARVTGE
HHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEECHHHHHHHCCC
MPELPRVMAARFEREDGLAAYDASMMTQSLAVARYYEATKAAGAPAKLAANWLMGELSRR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LNAEELGIDASPVSPATLAALIARIADGTISNNGAKQVFDALWNGETDVDAVISAKGLKQ
CCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
MSDSGELSAIVDAVLAANPKSVDEYRAGKDKAFNALVGQAMKASKGKANPTQLSELLKKK
HCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LG
CC
>Mature Secondary Structure
MNVAPALIRGYEVVIGLETHTQLSTASKIFSGAPTAFGAAPNTQACAVDLALPGSLPVMN
CCCCHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
RGAVERAIRFGLAVGGQIAPLSIFARKNYFYPDLPKGYQISQYELPVVQGGTVEFYLADV
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCEEECCEEEEEEECC
PHQVRLTRAHLEEDAGKSVHDAFDGLSGIDLNRAGTPLLEIVSEPDMRSSKEAVEYAKAL
CCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHH
HALVVWLGICDGNMQEGSFRCDANVSVRRPGAPFGTRREIKNLNSFRFLQQAIDFEIQWQ
HHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCHHHHHCCHHHHHHHHHHCEEEEEE
IDQIEDGLAIRQATVLFNPDSGETRAMRTKEDAHDYRYFPDPDLPPLVIAPEWIARVTGE
HHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEECHHHHHHHCCC
MPELPRVMAARFEREDGLAAYDASMMTQSLAVARYYEATKAAGAPAKLAANWLMGELSRR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LNAEELGIDASPVSPATLAALIARIADGTISNNGAKQVFDALWNGETDVDAVISAKGLKQ
CCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
MSDSGELSAIVDAVLAANPKSVDEYRAGKDKAFNALVGQAMKASKGKANPTQLSELLKKK
HCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA