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The map label for this gene is gatA [H]

Identifier: 124265278

GI number: 124265278

Start: 80781

End: 82271

Strand: Direct

Name: gatA [H]

Synonym: Mpe_A0085

Alternate gene names: 124265278

Gene position: 80781-82271 (Clockwise)

Preceding gene: 124265277

Following gene: 124265279

Centisome position: 2.0

GC content: 71.5

Gene sequence:

>1491_bases
ATGAGCACCGAGCTGCACCAGCTCGGCGTGGCCGACCTGTCGGCCCGTCTGGCCGCCAAGACGGTATCGACTGCCGAAGT
CACGTCACACCTGCTTGCGCGCGTTGCTGCCCATGAACACCTGGGGGCCTTCCTGTGCACCGATGTCGAACTGGCGCTGC
AGCAGGCCCGCGCGGCCGACGAGCGGCGCGCCCGTGGCGAGACCCTCGGCGCGCTCGACGGCGTGCCGCTGGCCCACAAG
GACATCTTCGTCACGACCGACTTTCCCAGCACCGCGGCCTCGAAGATGCTGGCCGGTTACCGCTCGCCCTTCGATGCAAC
GGTCGTCGCGAAGCTCAAGGCGGCCGGCACCGTGACCTTGGGCAAGCTCAACTGCGACGAGTTCGCGATGGGCGGCAGCA
ACGAGAACTCGGCCATCCGGCCGGTCCGCAACCCCTGGGACGCGGAGCGCATCCCGGGCGGGTCGTCGGGTGGCTCGGCG
GTGGCGGTGGCGGCGCGGCTGGTGCCGGCCACCACCGGCACCGACACCGGCGGCTCGGTGCGCCAGCCGGCCTCGCTGTC
CGGCGTGACCGGCATCAAGCCGACCTACGGGCGCTGCTCGCGCTACGGGATGATCGCCTTCGCCTCCAGCCTCGACCAGG
CAGGCCCGATCGCCAAGAGCGCCGAGGACTGCGCGCTGCTGCTGGGCGCGATGAGCGGCTTCGACGAGCGCGACGCCACC
TCGGCGCAGCAGCCGGTGCCCGACTTCGCCGCGCTGCTGCGTGCCCCGCGCGACGGTGCAAGCGCCGGCCGGCCGTTGCA
CGGCCTGCGCATCGGTCTGCCGCGGGAGTTCTTCGGTGCCGGCGTGGCCGGCGACGTGACGCAGGCGGTACGTGCTGCAC
TGGCCGAGGTCGAGAAGCTCGGCGCCACGTTGGTCGACGTGAGCCTGCCGCGCACCGAGCTGTCGATCCCGGTCTACTAC
ATCATCGCCCCCGCCGAGGCGAGCTCCAACCTGGGCCGCTTCGACGGCGTGCGCTACGGGCACCGCGCCGCGCAGTACGG
TGACCTGCTCGACATGTACCGCAAGAGCCGCAGCGAAGGCTTCGGGGCCGAGGTCAAGCGCCGCATCATGATCGGCACCT
ACGTGCTGAGCCACGGCTACTACGACGCCTACTACCTGCAGGCGCAGAAGCTGCGCCGCATGATCGCCGACGACTTCCAG
GCGGCCTTCGCCCACTGCGACCTGATCGCCGGTCCGGTGGCGCCGACCGTCGCGCGCCGGCTGGGTTCGCAGACCGACCC
GGTGGCCGAGTACCTGGCCGACATCTTCACGCTGCCGGCCAGCCTGGCCGGACTGCCGGGCATGAGCGCGCCGGCGGGAT
TCGGCGAGGGGGGGCTGCCGGTCGGCCTGCAGCTGATCGGCAACTACTGGCAGGAGGCGCAGCTGCTGCACGCCGCCCAC
AGCTTCCAGCAAGCCACCGATTTCCACCTCCGGACGCCGGAGGGCTACTGA

Upstream 100 bases:

>100_bases
TGCGCGAAGACGCGGTCACCGAGGTCGACCAGCGCCAGGCCAACCAACTCAGCGCACCGGCCGTCGAGGCGGGGCTGTAC
CTCGTGCCCAAGGTGATCGA

Downstream 100 bases:

>100_bases
CATGAACGTCGCCCCCGCGCTGATCCGTGGTTACGAAGTCGTCATCGGCCTCGAGACCCACACCCAGCTGTCCACCGCGT
CGAAGATCTTCTCCGGTGCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSTELHQLGVADLSARLAAKTVSTAEVTSHLLARVAAHEHLGAFLCTDVELALQQARAADERRARGETLGALDGVPLAHK
DIFVTTDFPSTAASKMLAGYRSPFDATVVAKLKAAGTVTLGKLNCDEFAMGGSNENSAIRPVRNPWDAERIPGGSSGGSA
VAVAARLVPATTGTDTGGSVRQPASLSGVTGIKPTYGRCSRYGMIAFASSLDQAGPIAKSAEDCALLLGAMSGFDERDAT
SAQQPVPDFAALLRAPRDGASAGRPLHGLRIGLPREFFGAGVAGDVTQAVRAALAEVEKLGATLVDVSLPRTELSIPVYY
IIAPAEASSNLGRFDGVRYGHRAAQYGDLLDMYRKSRSEGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKLRRMIADDFQ
AAFAHCDLIAGPVAPTVARRLGSQTDPVAEYLADIFTLPASLAGLPGMSAPAGFGEGGLPVGLQLIGNYWQEAQLLHAAH
SFQQATDFHLRTPEGY

Sequences:

>Translated_496_residues
MSTELHQLGVADLSARLAAKTVSTAEVTSHLLARVAAHEHLGAFLCTDVELALQQARAADERRARGETLGALDGVPLAHK
DIFVTTDFPSTAASKMLAGYRSPFDATVVAKLKAAGTVTLGKLNCDEFAMGGSNENSAIRPVRNPWDAERIPGGSSGGSA
VAVAARLVPATTGTDTGGSVRQPASLSGVTGIKPTYGRCSRYGMIAFASSLDQAGPIAKSAEDCALLLGAMSGFDERDAT
SAQQPVPDFAALLRAPRDGASAGRPLHGLRIGLPREFFGAGVAGDVTQAVRAALAEVEKLGATLVDVSLPRTELSIPVYY
IIAPAEASSNLGRFDGVRYGHRAAQYGDLLDMYRKSRSEGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKLRRMIADDFQ
AAFAHCDLIAGPVAPTVARRLGSQTDPVAEYLADIFTLPASLAGLPGMSAPAGFGEGGLPVGLQLIGNYWQEAQLLHAAH
SFQQATDFHLRTPEGY
>Mature_495_residues
STELHQLGVADLSARLAAKTVSTAEVTSHLLARVAAHEHLGAFLCTDVELALQQARAADERRARGETLGALDGVPLAHKD
IFVTTDFPSTAASKMLAGYRSPFDATVVAKLKAAGTVTLGKLNCDEFAMGGSNENSAIRPVRNPWDAERIPGGSSGGSAV
AVAARLVPATTGTDTGGSVRQPASLSGVTGIKPTYGRCSRYGMIAFASSLDQAGPIAKSAEDCALLLGAMSGFDERDATS
AQQPVPDFAALLRAPRDGASAGRPLHGLRIGLPREFFGAGVAGDVTQAVRAALAEVEKLGATLVDVSLPRTELSIPVYYI
IAPAEASSNLGRFDGVRYGHRAAQYGDLLDMYRKSRSEGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKLRRMIADDFQA
AFAHCDLIAGPVAPTVARRLGSQTDPVAEYLADIFTLPASLAGLPGMSAPAGFGEGGLPVGLQLIGNYWQEAQLLHAAHS
FQQATDFHLRTPEGY

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=34.251968503937, Blast_Score=302, Evalue=6e-82,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=25.1509054325956, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=37.3362445414847, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=252, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=143, Percent_Identity=32.8671328671329, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=247, Percent_Identity=28.7449392712551, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=198, Percent_Identity=28.2828282828283, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=438, Percent_Identity=36.986301369863, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=28.2758620689655, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=38.8329979879276, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24644968, Length=522, Percent_Identity=24.3295019157088, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=24.6601941747573, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=28.4552845528455, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=28.4552845528455, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=28.4552845528455, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356731, Length=183, Percent_Identity=34.4262295081967, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648441, Length=172, Percent_Identity=31.9767441860465, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648439, Length=172, Percent_Identity=31.9767441860465, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52234; Mature: 52102

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELHQLGVADLSARLAAKTVSTAEVTSHLLARVAAHEHLGAFLCTDVELALQQARAAD
CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRARGETLGALDGVPLAHKDIFVTTDFPSTAASKMLAGYRSPFDATVVAKLKAAGTVTL
HHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEE
GKLNCDEFAMGGSNENSAIRPVRNPWDAERIPGGSSGGSAVAVAARLVPATTGTDTGGSV
ECCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
RQPASLSGVTGIKPTYGRCSRYGMIAFASSLDQAGPIAKSAEDCALLLGAMSGFDERDAT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
SAQQPVPDFAALLRAPRDGASAGRPLHGLRIGLPREFFGAGVAGDVTQAVRAALAEVEKL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHH
GATLVDVSLPRTELSIPVYYIIAPAEASSNLGRFDGVRYGHRAAQYGDLLDMYRKSRSEG
CCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
FGAEVKRRIMIGTYVLSHGYYDAYYLQAQKLRRMIADDFQAAFAHCDLIAGPVAPTVARR
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LGSQTDPVAEYLADIFTLPASLAGLPGMSAPAGFGEGGLPVGLQLIGNYWQEAQLLHAAH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SFQQATDFHLRTPEGY
HHHHHCCCEEECCCCC
>Mature Secondary Structure 
STELHQLGVADLSARLAAKTVSTAEVTSHLLARVAAHEHLGAFLCTDVELALQQARAAD
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRARGETLGALDGVPLAHKDIFVTTDFPSTAASKMLAGYRSPFDATVVAKLKAAGTVTL
HHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEE
GKLNCDEFAMGGSNENSAIRPVRNPWDAERIPGGSSGGSAVAVAARLVPATTGTDTGGSV
ECCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
RQPASLSGVTGIKPTYGRCSRYGMIAFASSLDQAGPIAKSAEDCALLLGAMSGFDERDAT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
SAQQPVPDFAALLRAPRDGASAGRPLHGLRIGLPREFFGAGVAGDVTQAVRAALAEVEKL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHH
GATLVDVSLPRTELSIPVYYIIAPAEASSNLGRFDGVRYGHRAAQYGDLLDMYRKSRSEG
CCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
FGAEVKRRIMIGTYVLSHGYYDAYYLQAQKLRRMIADDFQAAFAHCDLIAGPVAPTVARR
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LGSQTDPVAEYLADIFTLPASLAGLPGMSAPAGFGEGGLPVGLQLIGNYWQEAQLLHAAH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SFQQATDFHLRTPEGY
HHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA