Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 124024579

GI number: 124024579

Start: 2556611

End: 2557000

Strand: Reverse

Name: gcvH [H]

Synonym: P9303_28911

Alternate gene names: 124024579

Gene position: 2557000-2556611 (Counterclockwise)

Preceding gene: 124024580

Following gene: 124024578

Centisome position: 95.32

GC content: 54.1

Gene sequence:

>390_bases
ATGGCTTTCCAATTCCCCGACCACTTTCGGTTTGCCGATACCCATGAATACGCCAGTTTGGATGGTGATTTGGTGCGGGT
CGGCATCAGTGCCTTTGCTGTTGATCAGTTGGGTGACATTGTGTTTGTGGATTTGCCGGAGGTGGGCGATCTGCTTAATC
GCGGCACCACTTTCGGGTCCGTTGAGTCAGTGAAAGCTGTCGAAGACTTGCATGCTCCGATCAGCGGTGAGTTGGTGCGG
ATTAATGAGTCGGTGCTTTCCAGCCCGGATGAACTTCAAAATGATCCGCATGGTGAGGGGTGGTTGCTCGTGGTTCGCCC
GGCGGACCCAGCTCAATTGCAGGATCTGATGGACGCAGCCACCTATGCCAACAAGGTGGCTGTCGAATAA

Upstream 100 bases:

>100_bases
CTCATGTGGAATTGGCACTGATCCGAGCTTTGGTTGCTTTGGTTCGCGCTGGTTTGCTGGATGTACCCCAGACTGGGTGA
GCTTTGCCTCGCCAGGCTCG

Downstream 100 bases:

>100_bases
ATCAACGAAGCACCCGATGAAGTGCGATGGTTCTGAGCCAAATTTTCTAAACTTCTGTCCATCTTGTTGATGAATGTCCC
TTGACCCTGCTTGAACAGCG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MAFQFPDHFRFADTHEYASLDGDLVRVGISAFAVDQLGDIVFVDLPEVGDLLNRGTTFGSVESVKAVEDLHAPISGELVR
INESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAATYANKVAVE

Sequences:

>Translated_129_residues
MAFQFPDHFRFADTHEYASLDGDLVRVGISAFAVDQLGDIVFVDLPEVGDLLNRGTTFGSVESVKAVEDLHAPISGELVR
INESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAATYANKVAVE
>Mature_128_residues
AFQFPDHFRFADTHEYASLDGDLVRVGISAFAVDQLGDIVFVDLPEVGDLLNRGTTFGSVESVKAVEDLHAPISGELVRI
NESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAATYANKVAVE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=38.9380530973451, Blast_Score=100, Evalue=3e-22,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=38.9380530973451, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789271, Length=118, Percent_Identity=38.135593220339, Blast_Score=99, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=129, Percent_Identity=35.6589147286822, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=34.2105263157895, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319272, Length=123, Percent_Identity=34.1463414634146, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=35.0877192982456, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14022; Mature: 13891

Theoretical pI: Translated: 3.96; Mature: 3.96

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQFPDHFRFADTHEYASLDGDLVRVGISAFAVDQLGDIVFVDLPEVGDLLNRGTTFGS
CCCCCCCCCCCCCCCCHHCCCCCEEEHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCC
VESVKAVEDLHAPISGELVRINESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAA
HHHHHHHHHHCCCCCCCEEEECHHHHCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
TYANKVAVE
HHHHHCCCC
>Mature Secondary Structure 
AFQFPDHFRFADTHEYASLDGDLVRVGISAFAVDQLGDIVFVDLPEVGDLLNRGTTFGS
CCCCCCCCCCCCCCCHHCCCCCEEEHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCC
VESVKAVEDLHAPISGELVRINESVLSSPDELQNDPHGEGWLLVVRPADPAQLQDLMDAA
HHHHHHHHHHCCCCCCCEEEECHHHHCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
TYANKVAVE
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]