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The map label for this gene is eno

Identifier: 124024460

GI number: 124024460

Start: 2439382

End: 2440677

Strand: Reverse

Name: eno

Synonym: P9303_27721

Alternate gene names: 124024460

Gene position: 2440677-2439382 (Counterclockwise)

Preceding gene: 124024470

Following gene: 124024456

Centisome position: 90.98

GC content: 53.94

Gene sequence:

>1296_bases
GTGATCGATTCCCTCGACCTCGTCATTGACACCATCGTGGCCAGAGAGGTTCTGGACTCCCGCGGCAACCCCACAGTTGA
AGCAGAAGTGTTGCTCGAAGCAGGTGCCATCGGTCGGGCCATCGTGCCCAGCGGAGCAAGCACCGGTGCCCATGAAGCGC
ATGAATTAAGAGATGGCGACAGCCGCTACATGGGCAAAGGGGTGACTAAGGCTGTGAACCATATCGAGGATCGGATTGCC
CCAGCGCTCTGTGGGATCTCCTCCCTAGATCAGGCCAGTGTTGATGGCACCATGCAAGAGCTTGATGGCAGCGACAACAA
ATCAAGCCTGGGCGCCAACGCGATCCTGGCCGTGAGCATGGCCACTGCCCGCGCGGCCGCTAATGGCTTGGGTTTGCCCC
TCTACCGCTACCTGGGTGGCCCCATGGCCTCATTGCTGCCCGTACCGCTGATGAATGTGATCAACGGTGGTGCGCATGCT
GCTAACAATCTCGATTTTCAGGAATTCATGCTGGTGCCCCATGGAGCAAGCACCTTCCGAGAATCGCTTCGCATGGGCGC
AGAAGTATTCCATACACTCAAGGGCCTGCTCAGTGCTCAGGGGCTCTCAACGGCAGTTGGAGATGAAGGCGGTTTTGCGC
CAAACCTGACCAACAATGATGCAGCAGGAGATCTGCTCATTCAAGCAATCGAAAAAGCAGGCTATTCGCCTGGTAAGGAT
ATTTCCTTGGCACTAGATGTAGCCAGCACAGAGTTCTATAAAGACGGCTGCTATGCCTTCGGGGGTGGGAGTTACACCAG
CACCGAAATGGTCAATGAGCTCGAAAAACTTGTTGATCGCTACCCAATTATTTCGATCGAAGATGGACTAGCAGAAGATG
ATTGGCAAGGTTGGGCTCTACTCACAAAAAAATTGGGGAAGCGTATCCAACTGATAGGTGACGATATTTTTGTAACTAGT
ACTAAGCGCCTACAACAGGGAATTGATCAAAACGTAGCCAATTCCATCTTGATTAAAGTGAATCAGATCGGCTCGCTAAC
CGAAACGCTTCAGGCCATTGATCTTGCTGGGCGCTCTGGTTACACCAGCGTGATCAGCCACCGCAGCGGCGAAACAGAAG
ACACCACAATTGCAGATCTTGCCGTTGCTACCCGTGCTGGGCAGATCAAAACGGGCTCCCTAAGTCGCAGTGAACGGGTA
GCGAAATACAACCAACTGCTGCGAATCGAAGACGAACTCGGTACTCAAGCGCTCTATGCCGGTGCCACAGGACAAGGGCC
ACGGGGCCGAAGCTAA

Upstream 100 bases:

>100_bases
CTACGACTGACAACAGCCTCAGCATCAGTAAAAGGGCTAGGCGGTGCACAGTCCGCGGGATGGCAAACCATAGGATCCAC
GCAACTTCAATTCGTCACAG

Downstream 100 bases:

>100_bases
GCACAGGCCTCAAAGGGTTGGACGGGGATCACTTTGGAATCGCTTCAGCGACTCTGACTCGACACATGATCCATGCGCGA
CTCACGTCCCAGCTTGATCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGDSRYMGKGVTKAVNHIEDRIA
PALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSMATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHA
ANNLDFQEFMLVPHGASTFRESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLTNNDAAGDLLIQAIEKAGYSPGKD
ISLALDVASTEFYKDGCYAFGGGSYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWALLTKKLGKRIQLIGDDIFVTS
TKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSGYTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERV
AKYNQLLRIEDELGTQALYAGATGQGPRGRS

Sequences:

>Translated_431_residues
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGDSRYMGKGVTKAVNHIEDRIA
PALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSMATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHA
ANNLDFQEFMLVPHGASTFRESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLTNNDAAGDLLIQAIEKAGYSPGKD
ISLALDVASTEFYKDGCYAFGGGSYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWALLTKKLGKRIQLIGDDIFVTS
TKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSGYTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERV
AKYNQLLRIEDELGTQALYAGATGQGPRGRS
>Mature_431_residues
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGDSRYMGKGVTKAVNHIEDRIA
PALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSMATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHA
ANNLDFQEFMLVPHGASTFRESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLTNNDAAGDLLIQAIEKAGYSPGKD
ISLALDVASTEFYKDGCYAFGGGSYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWALLTKKLGKRIQLIGDDIFVTS
TKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSGYTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERV
AKYNQLLRIEDELGTQALYAGATGQGPRGRS

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=51.8348623853211, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=439, Percent_Identity=50.7972665148064, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=439, Percent_Identity=50.7972665148064, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=50.4587155963303, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=437, Percent_Identity=45.9954233409611, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=28.3582089552239, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=28.3582089552239, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=28.3582089552239, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI310129182, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310110045, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310120572, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789141, Length=421, Percent_Identity=61.0451306413302, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=52.9680365296804, Blast_Score=423, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=52.9680365296804, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=196, Percent_Identity=48.9795918367347, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=50.1154734411085, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=48.2758620689655, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=48.2758620689655, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=48.2758620689655, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.4226327944573, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=442, Percent_Identity=48.868778280543, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=438, Percent_Identity=48.8584474885845, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=438, Percent_Identity=48.8584474885845, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PROM3 (A2CDE2)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018767.1
- ProteinModelPortal:   A2CDE2
- SMR:   A2CDE2
- STRING:   A2CDE2
- GeneID:   4776021
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_27721
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45424; Mature: 45424

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 339-339 BINDING 159-159 BINDING 168-168 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGD
CCCHHHHHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCCEECCCCCCCCCHHHHHHCCCC
SRYMGKGVTKAVNHIEDRIAPALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHHH
ATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHAANNLDFQEFMLVPHGASTFR
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHH
ESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLTNNDAAGDLLIQAIEKAGYSPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
ISLALDVASTEFYKDGCYAFGGGSYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWAL
CEEEEEEHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTKKLGKRIQLIGDDIFVTSTKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSG
HHHHHCCCEEEECCCEEEECHHHHHHHHHHHHHHHEEEEEHHHCHHHHHHHHHHHCCCCC
YTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERVAKYNQLLRIEDELGTQALYA
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GATGQGPRGRS
CCCCCCCCCCC
>Mature Secondary Structure
MIDSLDLVIDTIVAREVLDSRGNPTVEAEVLLEAGAIGRAIVPSGASTGAHEAHELRDGD
CCCHHHHHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCCEECCCCCCCCCHHHHHHCCCC
SRYMGKGVTKAVNHIEDRIAPALCGISSLDQASVDGTMQELDGSDNKSSLGANAILAVSM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHHH
ATARAAANGLGLPLYRYLGGPMASLLPVPLMNVINGGAHAANNLDFQEFMLVPHGASTFR
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHH
ESLRMGAEVFHTLKGLLSAQGLSTAVGDEGGFAPNLTNNDAAGDLLIQAIEKAGYSPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
ISLALDVASTEFYKDGCYAFGGGSYTSTEMVNELEKLVDRYPIISIEDGLAEDDWQGWAL
CEEEEEEHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTKKLGKRIQLIGDDIFVTSTKRLQQGIDQNVANSILIKVNQIGSLTETLQAIDLAGRSG
HHHHHCCCEEEECCCEEEECHHHHHHHHHHHHHHHEEEEEHHHCHHHHHHHHHHHCCCCC
YTSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERVAKYNQLLRIEDELGTQALYA
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GATGQGPRGRS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA