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The map label for this gene is trmE [H]

Identifier: 124024442

GI number: 124024442

Start: 2425258

End: 2426655

Strand: Reverse

Name: trmE [H]

Synonym: P9303_27541

Alternate gene names: 124024442

Gene position: 2426655-2425258 (Counterclockwise)

Preceding gene: 124024446

Following gene: 124024441

Centisome position: 90.46

GC content: 56.87

Gene sequence:

>1398_bases
ATGATGTCTTCTCCAATCCCCCATCAACAAACGATTGCCGCAGTGGCAACGGCGGTTGCACCAGGGCAAGGGGGTATTGC
TGTGGTGCGGCTTTCTGGGCCAGCGGCTGAGGTCGTTGGGCGGAGTGTGGTGTCGATCCCGGGGCAACAACTTTGGGTTA
GTCATCGTGTGTTGTATGGCCATGTGATGGATGAGAGCGGCAAGGAACGGATCGATGAAGTTCTTGTGTTGCTGATGAAG
GGGCCACGCAGTTTCACTGGCGAGGATGTTGTGGAGATCCACTGTCATGGTGGATTGATGGCTGTTCAGCGGGTGCTTGA
GCGTGTGTTGGCTCAGCCGCATGTGCGCCGGGCATTGCCTGGAGAGTTCAGCCAGCGGGCGGTGCTCAACGGCCGGCTTG
ACCTCACCCAGGCGGAGGCGATCAGTGAACTTGTGGCGGCGCGTAGTCGTCGAGCAGCCCAGTTGGCGATGACGGGAGTC
GATGGAGGCATTCAGCGCCGCATCACCTCCCTGCGGGAAAGGTTGTTGGATCAGCTCAGTGAGCTGGAGGCCAGGGTTGA
TTTTGAGGAGGATCTTCCCCCTCTCGATGCTGCTGAGTTGTTACTTGAGTTGCAGTGCGTGCGGCGAGAGCTCGAACAGC
TTGTTGAAGATGCCAAGCGTGGTGATGTGCTGCGTCAGGGGCTACAGGTGGCTCTGGTTGGTCGCCCTAATGTTGGTAAG
AGCTCCCTGTTGAATCGCCTCAGCCGCCGGGAGCGGGCGATCGTGACGGATCTTCCTGGTACGACCAGGGATGTCTTGGA
GAGCGAGATTGTGCTGGAGGGTGTGCCGATCACCTTGGTTGACACCGCAGGTATTCGCGCCACCAAAGATGCGTTGGAGC
AACTTGGGATAGACCGTAGTCATCAGGCTCTCGCTGCAGCTGATGTGGCGGTGTTGGTGTTTGATCTGAGTTTGGGATGG
ACAGCCGACGATGCGGCGTTGCTCGCTCAGATCCCAGATGATCTGCCAAGGCTTCTTGTGGGTAATAAAGCTGATCTTCA
GCCTGCCTCGATGACTGCTTCGCTTAGCAATGAGGTCGATGGCAAGACTGTGGATGTGATGTTGAGTGCACTCACTGGGC
AAGGAGAAGAGGCCTTGATTGAGGCTGTTTTGAAGACTTGTGGGGCCAGCGAAGCGCAGGGGTTGGTGGTTGCTCTCAAT
CAACGGCAGCAAGATCTTGCCGCAACAGCAGCAATTGCCTTGGCTCGCACGCAGGAAGCTGCAGCGCAGAAGTTGCCCTG
GGATTTCTGGACCATTGATCTGCGGCAAGCGATCAGCAGCTTGGGTGAGATCACTGGCGAGGAGATCACAGAAGCTGTGC
TTGATCGAATCTTTTCGCGGTTTTGTATTGGCAAATAA

Upstream 100 bases:

>100_bases
CGCCTGCTGATGCCCTGGAGGGTTTGCTGGATCATGGCCAGACCGAAGCAATGGTGCTGCAGGGTGGCGTTGATCGTAGG
AAGGGCTGAAGGTACCAGTG

Downstream 100 bases:

>100_bases
GTGAAAATAGAAACGCACTAGTGTGAATCAATAGTGGCAAAATGCTTTGTTCTGCCTAGCCAATCACGCTTCGAAAGATT
GTATTTTTGTTTGCAAGATG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYGHVMDESGKERIDEVLVLLMK
GPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALPGEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGV
DGGIQRRITSLRERLLDQLSELEARVDFEEDLPPLDAAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATKDALEQLGIDRSHQALAAADVAVLVFDLSLGW
TADDAALLAQIPDDLPRLLVGNKADLQPASMTASLSNEVDGKTVDVMLSALTGQGEEALIEAVLKTCGASEAQGLVVALN
QRQQDLAATAAIALARTQEAAAQKLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK

Sequences:

>Translated_465_residues
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYGHVMDESGKERIDEVLVLLMK
GPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALPGEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGV
DGGIQRRITSLRERLLDQLSELEARVDFEEDLPPLDAAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATKDALEQLGIDRSHQALAAADVAVLVFDLSLGW
TADDAALLAQIPDDLPRLLVGNKADLQPASMTASLSNEVDGKTVDVMLSALTGQGEEALIEAVLKTCGASEAQGLVVALN
QRQQDLAATAAIALARTQEAAAQKLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK
>Mature_465_residues
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYGHVMDESGKERIDEVLVLLMK
GPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALPGEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGV
DGGIQRRITSLRERLLDQLSELEARVDFEEDLPPLDAAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATKDALEQLGIDRSHQALAAADVAVLVFDLSLGW
TADDAALLAQIPDDLPRLLVGNKADLQPASMTASLSNEVDGKTVDVMLSALTGQGEEALIEAVLKTCGASEAQGLVVALN
QRQQDLAATAAIALARTQEAAAQKLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=473, Percent_Identity=35.3065539112051, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI193082993, Length=465, Percent_Identity=35.0537634408602, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=33.2007952286282, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=38.2608695652174, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI87082120, Length=173, Percent_Identity=35.2601156069364, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=31.3304721030043, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=508, Percent_Identity=30.9055118110236, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45550813, Length=469, Percent_Identity=32.409381663113, Blast_Score=195, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50036; Mature: 50036

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHCCCHHHHHHHHHHHH
HVMDESGKERIDEVLVLLMKGPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALP
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHCCC
GEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGVDGGIQRRITSLRERLLDQLS
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHH
ELEARVDFEEDLPPLDAAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATKDALEQLGIDRS
HHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCC
HQALAAADVAVLVFDLSLGWTADDAALLAQIPDDLPRLLVGNKADLQPASMTASLSNEVD
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCC
GKTVDVMLSALTGQGEEALIEAVLKTCGASEAQGLVVALNQRQQDLAATAAIALARTQEA
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
AAQKLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHCCCHHHHHHHHHHHH
HVMDESGKERIDEVLVLLMKGPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALP
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHCCC
GEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGVDGGIQRRITSLRERLLDQLS
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHH
ELEARVDFEEDLPPLDAAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATKDALEQLGIDRS
HHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCC
HQALAAADVAVLVFDLSLGWTADDAALLAQIPDDLPRLLVGNKADLQPASMTASLSNEVD
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCC
GKTVDVMLSALTGQGEEALIEAVLKTCGASEAQGLVVALNQRQQDLAATAAIALARTQEA
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
AAQKLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]