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The map label for this gene is trpB [H]

Identifier: 124024393

GI number: 124024393

Start: 2387346

End: 2388611

Strand: Reverse

Name: trpB [H]

Synonym: P9303_27051

Alternate gene names: 124024393

Gene position: 2388611-2387346 (Counterclockwise)

Preceding gene: 124024394

Following gene: 124024392

Centisome position: 89.04

GC content: 56.71

Gene sequence:

>1266_bases
TTGCCTGGCGTACCGGTGACCAGCACTTTGCCCTCTCAGCCCAAAGATGCGGACCTCGCTGTGAGTGCGCGACCTTCTGC
CCAGGGGCGTTTCGGCAGATATGGGGGCCAGTACGTGCCCGAAACCTTGATGCCTGCCTTGGCTGAACTGGAGCAAGCAG
CTGCGCTGGCATGGAAAGATCCTGCCTTCACTGCTGAGTTGGATCGGCTGCTGCGTAGCTATGTGGGTCGGGCGACGCCG
CTTTATGAGGCTGAGCGCCTCACAGCTCATTACTGCCGTTCAGACGGAGGACCCAGGATCTGGCTGAAGCGTGAGGATCT
CAATCACACAGGCGCCCACAAAATCAATAACGCTTTGGGTCAGGCTCTGTTGGCGTTACGCATGGGTAAAAAGCGGATCA
TTGCCGAAACAGGTGCGGGTCAACACGGTGTGGCCACGGCCACGGTGTGTGCCCGCTTTGGTCTTGAGTGTGTTGTTTAC
ATGGGCGCTGAGGACATGCGCCGTCAGGCGTTGAATGTGTTCCGCATGCGTCTGCTTGGGGCCACAGTGCAGCCTGTCAC
TGCAGGCACGGCAACGCTGAAGGACGCGACGAGTGAGGCGATTCGTGATTGGGTCACCAATGTGGAAACCACCCATTACA
TCCTTGGTTCGGTGGCTGGTCCCCATCCCTATCCGATGCTTGTTCGCGACTTTCATGCCGTGATCGGTCAGGAGGCCAGG
CAGCAGTGTGCTGAGGCTTTCGGTCGCTTGCCTGATGTTCTTTTGGCGTGCGTTGGTGGAGGGTCCAATGCCATGGGTTT
GTTTCATCCCTTCGTGCAGGAGCGCTCTGTGCGCATGATTGGAGTTGAAGCTGCTGGTGATGGTGTTGAAACTGGTCGTC
ATGCGGCAACAATCACCGAAGGTCGAGTAGGCGTCTTGCATGGAGCGATGAGTTTGTTGCTTCAGGATGATGAAGGTCAG
GTGCAAGAAGCCCATTCGATTAGTGCCGGCCTCGACTACCCGGGAGTTGGTCCAGAACACAGCTACTTATGTGAGATTGG
TCGGGCGGAGTATGCCGCGGTTAGCGATCAGCAGGCTTTGGATGCCTTGCGCTTGGTGAGTGAACTAGAGGGAATTATTC
CTGCTTTAGAGACGGCCCATGCCTTTGCTTGGTTAGAGACGCTTTGCCCCACTCTTGCCGCTGGAACAGAAGTGGTGATC
AATTGCTCAGGCCGCGGCGATAAGGATGTCAACACCGTGGCAGAGAAACTTGGCAATCAGCTTTGA

Upstream 100 bases:

>100_bases
TGAGTTGGCTGGCAAAGACTCCCTCTAACTTTGCGGCTTCAAACGAAAGCACTCTTTGCTGCTTGGGGTGATTCTTTCGA
TTCCTAGGCTGGTGTTGTTC

Downstream 100 bases:

>100_bases
GTGATGGCTGCCGGCAGTCGCTAAGGCGAGGGTTTTGATCTAGGAATTTTCCTTGCCTGCCAACCCAACGACTGCCTCAC
GCTCTGCTCTGCAAGCCAGT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKDPAFTAELDRLLRSYVGRATP
LYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVY
MGAEDMRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMLVRDFHAVIGQEAR
QQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQERSVRMIGVEAAGDGVETGRHAATITEGRVGVLHGAMSLLLQDDEGQ
VQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVI
NCSGRGDKDVNTVAEKLGNQL

Sequences:

>Translated_421_residues
MPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKDPAFTAELDRLLRSYVGRATP
LYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVY
MGAEDMRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMLVRDFHAVIGQEAR
QQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQERSVRMIGVEAAGDGVETGRHAATITEGRVGVLHGAMSLLLQDDEGQ
VQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVI
NCSGRGDKDVNTVAEKLGNQL
>Mature_420_residues
PGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKDPAFTAELDRLLRSYVGRATPL
YEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALGQALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVYM
GAEDMRRQALNVFRMRLLGATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMLVRDFHAVIGQEARQ
QCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQERSVRMIGVEAAGDGVETGRHAATITEGRVGVLHGAMSLLLQDDEGQV
QEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVIN
CSGRGDKDVNTVAEKLGNQL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=388, Percent_Identity=53.6082474226804, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6321412, Length=396, Percent_Identity=60.1010101010101, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 44985; Mature: 44853

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKD
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PAFTAELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALG
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
QALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVYMGAEDMRRQALNVFRMRLLG
HHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHC
ATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMLVRDFHAVIGQEAR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQERSVRMIGVEAAGDGVETGRHAATITE
HHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEECC
GRVGVLHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQAL
CCHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHCCHHHHH
DALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVINCSGRGDKDVNTVAEKLGNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCC
L
C
>Mature Secondary Structure 
PGVPVTSTLPSQPKDADLAVSARPSAQGRFGRYGGQYVPETLMPALAELEQAAALAWKD
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PAFTAELDRLLRSYVGRATPLYEAERLTAHYCRSDGGPRIWLKREDLNHTGAHKINNALG
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
QALLALRMGKKRIIAETGAGQHGVATATVCARFGLECVVYMGAEDMRRQALNVFRMRLLG
HHHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHC
ATVQPVTAGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMLVRDFHAVIGQEAR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QQCAEAFGRLPDVLLACVGGGSNAMGLFHPFVQERSVRMIGVEAAGDGVETGRHAATITE
HHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEECC
GRVGVLHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQAL
CCHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHCCHHHHH
DALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVINCSGRGDKDVNTVAEKLGNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]