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The map label for this gene is metF [H]

Identifier: 124024370

GI number: 124024370

Start: 2369320

End: 2370216

Strand: Reverse

Name: metF [H]

Synonym: P9303_26821

Alternate gene names: 124024370

Gene position: 2370216-2369320 (Counterclockwise)

Preceding gene: 124024372

Following gene: 124024369

Centisome position: 88.35

GC content: 53.62

Gene sequence:

>897_bases
TTGAGTTCAGCCTTGCAGCGCTCCATCGAGGCCGGAGCGGTGACGATCACTGCTGAGGTCATGCCTCCTCGTGGTGCAGA
TCCTGTTCATTCCTTGACGATGGCTCAAGGTTTGAAAGGTTTTGTTCATGCCATCAATGTGACTGATGGCAGCCGTGCAG
TGATGCGGATGAGCAGCCTTGCTCTCTGCCGGTTGCTGTTAGATGAGGGGCTTGAACCGATTTTGCAGCTCGCTTGCCGT
GATCGCAATCGGATCGCCCTTCAATCAGATTTACTTGGCGCTCATGCCCTGGGTATTCGCAACTTGCTCTGCCTTACAGG
TGATCCTGTCCGGGTTGGTGATCAACCTGAGGCGAGACCTGTTCATGACTATGAGTCGGTGAAATTGCTGCATCAGGTCG
CTGCCTTCAACCGTGCGGAGGATCCTGTTTCGGGATTACTCCCTGATGGAGCGACTGCTTTGTTTGCTGGGGCCGCAGCT
GATCCCAATTGCCAGAGCTTGAGTGGTCTGCGTCGTCGCCTCGAACGAAAGAAGGAGGCCGGCGCTCGCTTTCTTCAGAC
TCAGATGGTGATGAAACCCGCTGTGCTTGAACACTTTTGCAATGAGGTGGCCAATCCCCTTGGGTTGCCTGTGCTTGCTG
GGGTGTTTTTGCTGAAATCAGCTCGTAATGCAAGCTTCATTAATAGGGTTGTGCCTGGAGCATGCATTCCTGAATCACTT
GTGGTTCGGCTGGAGCAAGCATCTGATCCAATGGCTGAGGGGATCGCTATTGCTGCTGAGCAGGTCAGCAGTTACTTGTC
TCTTACCCAGGGCGTCCATCTGATGGCCGTGAAAGCAGAAGAGCGCATCCCCGAGATTCTTGATCAGGCTGGGATAAAGA
AATTGATGGATCCGTAG

Upstream 100 bases:

>100_bases
CTGTGGCCATTTCCTCCCCATTCATGATCTCCGGTCATCTTACGCAGACTTTGGGCAAGCGATCCCTCATAGTTGGTGAG
TGAGTGAAGGACATCGTGAT

Downstream 100 bases:

>100_bases
CATTAAATTCAGTCATACCAACTAATCTCGAGAATTGCGCTAAGATTTGGGGACGTCCCTGATTCATCCCCGAATGAGTC
CTGATCAAGTAATTCATGGA

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSSALQRSIEAGAVTITAEVMPPRGADPVHSLTMAQGLKGFVHAINVTDGSRAVMRMSSLALCRLLLDEGLEPILQLACR
DRNRIALQSDLLGAHALGIRNLLCLTGDPVRVGDQPEARPVHDYESVKLLHQVAAFNRAEDPVSGLLPDGATALFAGAAA
DPNCQSLSGLRRRLERKKEAGARFLQTQMVMKPAVLEHFCNEVANPLGLPVLAGVFLLKSARNASFINRVVPGACIPESL
VVRLEQASDPMAEGIAIAAEQVSSYLSLTQGVHLMAVKAEERIPEILDQAGIKKLMDP

Sequences:

>Translated_298_residues
MSSALQRSIEAGAVTITAEVMPPRGADPVHSLTMAQGLKGFVHAINVTDGSRAVMRMSSLALCRLLLDEGLEPILQLACR
DRNRIALQSDLLGAHALGIRNLLCLTGDPVRVGDQPEARPVHDYESVKLLHQVAAFNRAEDPVSGLLPDGATALFAGAAA
DPNCQSLSGLRRRLERKKEAGARFLQTQMVMKPAVLEHFCNEVANPLGLPVLAGVFLLKSARNASFINRVVPGACIPESL
VVRLEQASDPMAEGIAIAAEQVSSYLSLTQGVHLMAVKAEERIPEILDQAGIKKLMDP
>Mature_297_residues
SSALQRSIEAGAVTITAEVMPPRGADPVHSLTMAQGLKGFVHAINVTDGSRAVMRMSSLALCRLLLDEGLEPILQLACRD
RNRIALQSDLLGAHALGIRNLLCLTGDPVRVGDQPEARPVHDYESVKLLHQVAAFNRAEDPVSGLLPDGATALFAGAAAD
PNCQSLSGLRRRLERKKEAGARFLQTQMVMKPAVLEHFCNEVANPLGLPVLAGVFLLKSARNASFINRVVPGACIPESLV
VRLEQASDPMAEGIAIAAEQVSSYLSLTQGVHLMAVKAEERIPEILDQAGIKKLMDP

Specific function: Unknown

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 31885; Mature: 31754

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSALQRSIEAGAVTITAEVMPPRGADPVHSLTMAQGLKGFVHAINVTDGSRAVMRMSSL
CCHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
ALCRLLLDEGLEPILQLACRDRNRIALQSDLLGAHALGIRNLLCLTGDPVRVGDQPEARP
HHHHHHHHHCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
VHDYESVKLLHQVAAFNRAEDPVSGLLPDGATALFAGAAADPNCQSLSGLRRRLERKKEA
CCHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GARFLQTQMVMKPAVLEHFCNEVANPLGLPVLAGVFLLKSARNASFINRVVPGACIPESL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHH
VVRLEQASDPMAEGIAIAAEQVSSYLSLTQGVHLMAVKAEERIPEILDQAGIKKLMDP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSALQRSIEAGAVTITAEVMPPRGADPVHSLTMAQGLKGFVHAINVTDGSRAVMRMSSL
CHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
ALCRLLLDEGLEPILQLACRDRNRIALQSDLLGAHALGIRNLLCLTGDPVRVGDQPEARP
HHHHHHHHHCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
VHDYESVKLLHQVAAFNRAEDPVSGLLPDGATALFAGAAADPNCQSLSGLRRRLERKKEA
CCHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
GARFLQTQMVMKPAVLEHFCNEVANPLGLPVLAGVFLLKSARNASFINRVVPGACIPESL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHH
VVRLEQASDPMAEGIAIAAEQVSSYLSLTQGVHLMAVKAEERIPEILDQAGIKKLMDP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]