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The map label for this gene is rffG [C]

Identifier: 124024227

GI number: 124024227

Start: 2231844

End: 2232866

Strand: Reverse

Name: rffG [C]

Synonym: P9303_25381

Alternate gene names: 124024227

Gene position: 2232866-2231844 (Counterclockwise)

Preceding gene: 124024228

Following gene: 124024225

Centisome position: 83.23

GC content: 52.1

Gene sequence:

>1023_bases
ATGAGTCGATGCGTTGTTGTTAGCGGTGCTGCAGGATTCATTGGTGCTGCCCTCGTGCAGAGGCTGTTAGCCCAGGGTGA
CCGTGTTATTGGCATTGACAACCTGAATGACTACTACGATCCGTCTTTGAAGCGGGCACGACTTGCTCAGATTGAAGCGG
CTTCCCTTAAAGCGTCTTGGACTTTTTATCCAATAGCCTTGGAAGATGGTGCAGGCATAGAGGAACTGTTTAAGGCAGAG
AAGCCTCAGGTGGTCGTTAATTTGGCGGCTCAGGCAGGGGTTCGCTACTCATTGGAGAATCCGGCGGCCTACATTCAGGC
CAATTTGGTGGGCTTCGGTCACATCCTTGAGGGATGTCGTCATCATGGAGTGCAGCACTTGGTGTATGCCTCCAGTAGCT
CGGTATATGGTGGCAATCGCAACTTGCCTTTTCACGAGCAACAGCCTGTGAATCACCCGGTGAGTCTCTATGCAGCTACC
AAGAAGGCCAACGAATTGATGGCTCATACCTATAGCCATTTGTATGGGTTGCCTGCTACAGGATTGCGCTTTTTTACTGT
GTACGGACCTTGGGGCCGCCCGGATATGGCTCCGATGCTCTTCGCCAAGGCGATTCTTGCTGGCGAGCCCATCAAGGTGT
TCAATTACGGCAAGATGCAGCGTGATTTCACCTATATCGACGATATCGTTGAAGGGCTACTTCGTTGTTGCGACAAGCCT
GCTACCGCAAATTTAGGCTTCGATCCGCTGAACCCTGATCCAGCCACCGCTGCTGTACCCCACCGTCTTTTCAATATTGG
TAATAGCCAGCCCATTGAGTTGATGCATTTTATTGAGCTCTTGGAGAAGTCTCTCGGGAGGGAAGCGGTGAAGGACTTCC
AGCCGATGCAACCTGGTGATGTGATGGCCACCGCTGCTGATACATCTGCTCTTGAGGCCTGGGTGAATTTTCGGCCGTCG
ACCCCAATCGCAGAGGGGGTGGAGTGCTTCTCTCAGTGGTATCGAAGCTTTTATGAGGTTTGA

Upstream 100 bases:

>100_bases
TGCGTCAACCTGCCTGGGTGTTTGATGCTCGCGCTGTTGTCGATCCAGAAGCTGTGAAATCGTCTGGTTTGATGCTTTGG
CGAGTTGGAGATGGTGCTGT

Downstream 100 bases:

>100_bases
TCGATAGCTTGACATGCATGTACATGTAGGCGTTAACGCACCATCGCCGCAACATTTCCGCCGTCTACTGTCAGTAATGC
ACCAGTGGTTCGTGGCATTA

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSRCVVVSGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQIEAASLKASWTFYPIALEDGAGIEELFKAE
KPQVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCRHHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAAT
KKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
ATANLGFDPLNPDPATAAVPHRLFNIGNSQPIELMHFIELLEKSLGREAVKDFQPMQPGDVMATAADTSALEAWVNFRPS
TPIAEGVECFSQWYRSFYEV

Sequences:

>Translated_340_residues
MSRCVVVSGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQIEAASLKASWTFYPIALEDGAGIEELFKAE
KPQVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCRHHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAAT
KKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
ATANLGFDPLNPDPATAAVPHRLFNIGNSQPIELMHFIELLEKSLGREAVKDFQPMQPGDVMATAADTSALEAWVNFRPS
TPIAEGVECFSQWYRSFYEV
>Mature_339_residues
SRCVVVSGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQIEAASLKASWTFYPIALEDGAGIEELFKAEK
PQVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCRHHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAATK
KANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKPA
TANLGFDPLNPDPATAAVPHRLFNIGNSQPIELMHFIELLEKSLGREAVKDFQPMQPGDVMATAADTSALEAWVNFRPST
PIAEGVECFSQWYRSFYEV

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=25.7971014492754, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=24.2339832869081, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=24.2339832869081, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=24.2339832869081, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=22.6086956521739, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI193211614, Length=300, Percent_Identity=25.6666666666667, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI8393516, Length=300, Percent_Identity=25.6666666666667, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=26.4957264957265, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=23.0555555555556, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=23.0547550432277, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=24.8587570621469, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=24.7191011235955, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=25.297619047619, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=248, Percent_Identity=27.8225806451613, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=24.9283667621776, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37396; Mature: 37265

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCVVVSGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQIEAASLKASW
CCCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEECE
TFYPIALEDGAGIEELFKAEKPQVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCR
EEEEEEECCCCCHHHHHCCCCCEEEEEEHHHCCCEEECCCCCEEEEEHHHHHHHHHHHHH
HHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAATKKANELMAHTYSHLYGLPAT
HCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
ATANLGFDPLNPDPATAAVPHRLFNIGNSQPIELMHFIELLEKSLGREAVKDFQPMQPGD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCCC
VMATAADTSALEAWVNFRPSTPIAEGVECFSQWYRSFYEV
EEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRCVVVSGAAGFIGAALVQRLLAQGDRVIGIDNLNDYYDPSLKRARLAQIEAASLKASW
CCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEECE
TFYPIALEDGAGIEELFKAEKPQVVVNLAAQAGVRYSLENPAAYIQANLVGFGHILEGCR
EEEEEEECCCCCHHHHHCCCCCEEEEEEHHHCCCEEECCCCCEEEEEHHHHHHHHHHHHH
HHGVQHLVYASSSSVYGGNRNLPFHEQQPVNHPVSLYAATKKANELMAHTYSHLYGLPAT
HCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLRFFTVYGPWGRPDMAPMLFAKAILAGEPIKVFNYGKMQRDFTYIDDIVEGLLRCCDKP
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
ATANLGFDPLNPDPATAAVPHRLFNIGNSQPIELMHFIELLEKSLGREAVKDFQPMQPGD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCCC
VMATAADTSALEAWVNFRPSTPIAEGVECFSQWYRSFYEV
EEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]