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The map label for this gene is ndhK

Identifier: 124024214

GI number: 124024214

Start: 2222630

End: 2223382

Strand: Reverse

Name: ndhK

Synonym: P9303_25251

Alternate gene names: 124024214

Gene position: 2223382-2222630 (Counterclockwise)

Preceding gene: 124024215

Following gene: 124024213

Centisome position: 82.88

GC content: 54.18

Gene sequence:

>753_bases
ATGTCAGTGACCCCCTCCATCGATGCCGTACGTGACTTGCGTGCGGCGAGTTGTGGCCCTGTCGGCGCACCCAGTGTGAC
CAGTGAATTGAGTGAGAACATCATTCTCACAAGCCTGGACGATCTACACAATTGGGCTCGGCTTAGCAGTCTCTGGCCGT
TGCTGTATGGCACCGCGTGTTGCTTTATCGAGTTCGCGGCTCTGATTGGATCACGTTTTGATTTTGACCGTTTTGGATTG
GTGCCCCGAAGCTCTCCTCGGCAGGCTGATCTCCTCATCGTTGCTGGCACGGTGACCATGAAGATGGCTCCAGCCCTGGT
CAGGCTTTATGAGCAGATGCCTGAGCCTAAATACGTGATTGCCATGGGTGCATGCACGATCACTGGTGGCATGTTCAGTG
CTGACTCCACTACCGCTGTGCGTGGAGTAGACAAGCTCATCCCCGTAGATCTGTATCTCCCAGGGTGCCCACCTCGCCCT
GAAGCAATTTTTGATGCGGTGATCAAGTTACGCAAAAAAGTGGGTGATGAATCGGTCTCTGAGCGGATCAAAATTGCCCA
GACCCACCGCTACTTCACAGTGCCCCATCAAATGAAGCGAGTTGAGCCAATTGTCACCGGGGCCTACTTGAGTGCTGATA
CCCAGAAAGCTGCTTTGTCGCCTGGTGCTGGGCTGCCAATGGCTGCGGAGTTGAACACCTCTGAAATTGATGCCAGCCCA
GCTTCTCAGCCTTCATCAACCTACGAGTCATGA

Upstream 100 bases:

>100_bases
GGTCTATTGGCTTTCATCGAAGCCCTTATTTTTATTGCCATCCTTCTTGTTGCACTGGCTTATGCCTGGCGGAAGGGTGC
CCTCGAGTGGAGTTGATTTC

Downstream 100 bases:

>100_bases
GCGAGACCCCTTCCACCCCCACGGTGCCGGCGGGTCCGGAACCTGGACCTATAAGCAGATGGCTCAACAAGCAGGGTTTT
GAGCATCAGTTGCTTGATCC

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSVTPSIDAVRDLRAASCGPVGAPSVTSELSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGDESVSERIKIAQTHRYFTVPHQMKRVEPIVTGAYLSADTQKAALSPGAGLPMAAELNTSEIDASP
ASQPSSTYES

Sequences:

>Translated_250_residues
MSVTPSIDAVRDLRAASCGPVGAPSVTSELSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGDESVSERIKIAQTHRYFTVPHQMKRVEPIVTGAYLSADTQKAALSPGAGLPMAAELNTSEIDASP
ASQPSSTYES
>Mature_249_residues
SVTPSIDAVRDLRAASCGPVGAPSVTSELSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLV
PRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPE
AIFDAVIKLRKKVGDESVSERIKIAQTHRYFTVPHQMKRVEPIVTGAYLSADTQKAALSPGAGLPMAAELNTSEIDASPA
SQPSSTYES

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=52.4475524475524, Blast_Score=177, Evalue=9e-45,
Organism=Escherichia coli, GI1788624, Length=153, Percent_Identity=49.6732026143791, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=42.5925925925926, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=49.6732026143791, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=55.3956834532374, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=55.3956834532374, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=55.1470588235294, Blast_Score=170, Evalue=7e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_PROM3 (A2CCP6)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018521.1
- ProteinModelPortal:   A2CCP6
- SMR:   A2CCP6
- STRING:   A2CCP6
- GeneID:   4778323
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_25251
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- ProtClustDB:   PRK06411
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 26879; Mature: 26748

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTPSIDAVRDLRAASCGPVGAPSVTSELSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGDESVS
EECCEEEECCCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHH
ERIKIAQTHRYFTVPHQMKRVEPIVTGAYLSADTQKAALSPGAGLPMAAELNTSEIDASP
HHHHHHHHCCEEECCHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCEEECCCCHHCCCCC
ASQPSSTYES
CCCCCCCCCC
>Mature Secondary Structure 
SVTPSIDAVRDLRAASCGPVGAPSVTSELSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGDESVS
EECCEEEECCCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHH
ERIKIAQTHRYFTVPHQMKRVEPIVTGAYLSADTQKAALSPGAGLPMAAELNTSEIDASP
HHHHHHHHCCEEECCHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCEEECCCCHHCCCCC
ASQPSSTYES
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA