Click here to switch to the map view.

The map label for this gene is ndhJ

Identifier: 124024213

GI number: 124024213

Start: 2222076

End: 2222633

Strand: Reverse

Name: ndhJ

Synonym: P9303_25241

Alternate gene names: 124024213

Gene position: 2222633-2222076 (Counterclockwise)

Preceding gene: 124024214

Following gene: 124024211

Centisome position: 82.85

GC content: 53.58

Gene sequence:

>558_bases
ATGAGCGAGACCCCTTCCACCCCCACGGTGCCGGCGGGTCCGGAACCTGGACCTATAAGCAGATGGCTCAACAAGCAGGG
TTTTGAGCATCAGTTGCTTGATCCTGATCATCTTGGTGTGGAGGTGATTGGTGTTGAGTCGATGTTCCTGCAGGTGATCG
TTGCAGCTCTCAAGGCTGATGGTTTTGATTATCTTCAATGCCAGGGTGGCTATGACGAAGGCCCTGGCCAGCAGCTGGTC
TGCTTCTACCATCTCGTGGCCATGGCTGAAATGGTTGCAAAGATGGGGGTGGGAGATTCGTCGTCAGAAGCGGCCAAGGT
CCGTGAGGTACGCCTCAAGGTGTTTTTATCCCGTGAAGACACACCTTCGCTCCCATCGATCTACGGCTTGTTTCGCGGAG
CTGACTGGCAGGAGCGGGAAACCTTTGACATGTTCGGCATCAACTTTGAGGGACATCCCCATCCGAAAAGGTTGCTAATG
CCTGAAGACTGGAAGGGCTGGCCACTGCGGAAAGATTATGTGCAGCCAGACTTTTATGAGATGCAGGATGCTTATTGA

Upstream 100 bases:

>100_bases
CTGCTTTGTCGCCTGGTGCTGGGCTGCCAATGGCTGCGGAGTTGAACACCTCTGAAATTGATGCCAGCCCAGCTTCTCAG
CCTTCATCAACCTACGAGTC

Downstream 100 bases:

>100_bases
GCATTCTTTTTATGCTTTCGATAGAAGCGCATCACTGCTAGGGCAAGGCTGCGTGGGTCATGGCGCAGAGTTGCGCGAGG
CCTTTTCCCTTGCAATGGAG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSETPSTPTVPAGPEPGPISRWLNKQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKADGFDYLQCQGGYDEGPGQQLV
CFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSREDTPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLM
PEDWKGWPLRKDYVQPDFYEMQDAY

Sequences:

>Translated_185_residues
MSETPSTPTVPAGPEPGPISRWLNKQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKADGFDYLQCQGGYDEGPGQQLV
CFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSREDTPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLM
PEDWKGWPLRKDYVQPDFYEMQDAY
>Mature_184_residues
SETPSTPTVPAGPEPGPISRWLNKQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKADGFDYLQCQGGYDEGPGQQLVC
FYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSREDTPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLMP
EDWKGWPLRKDYVQPDFYEMQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family

Homologues:

Organism=Escherichia coli, GI145693162, Length=99, Percent_Identity=40.4040404040404, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHJ_PROM3 (A2CCP5)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018520.1
- ProteinModelPortal:   A2CCP5
- SMR:   A2CCP5
- STRING:   A2CCP5
- GeneID:   4778153
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_25241
- eggNOG:   COG0852
- HOGENOM:   HBG727675
- OMA:   YELQDAY
- ProtClustDB:   PRK12494
- GO:   GO:0006810
- HAMAP:   MF_01357
- InterPro:   IPR010218
- InterPro:   IPR001268
- InterPro:   IPR020396
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa

EC number: 1.6.5.3

Molecular weight: Translated: 20885; Mature: 20754

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETPSTPTVPAGPEPGPISRWLNKQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKAD
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCC
GFDYLQCQGGYDEGPGQQLVCFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSRED
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCC
TPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLMPEDWKGWPLRKDYVQPDFYE
CCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
MQDAY
HCCCC
>Mature Secondary Structure 
SETPSTPTVPAGPEPGPISRWLNKQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKAD
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCC
GFDYLQCQGGYDEGPGQQLVCFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSRED
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCC
TPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLMPEDWKGWPLRKDYVQPDFYE
CCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
MQDAY
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA