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The map label for this gene is rfe [H]

Identifier: 124024160

GI number: 124024160

Start: 2172154

End: 2173302

Strand: Reverse

Name: rfe [H]

Synonym: P9303_24711

Alternate gene names: 124024160

Gene position: 2173302-2172154 (Counterclockwise)

Preceding gene: 124024161

Following gene: 124024159

Centisome position: 81.01

GC content: 54.83

Gene sequence:

>1149_bases
TTGACTCTCGCGAGCAGCCCCTTTGCTTTTGCTACGGCGAGCTTCGTGATGGCTGCTGTGCTTACAACTTTGGTTGTCCC
ACTCGTGCGGGCGCTAGGCCTTCATCTCGGTCTCACTGATCAGCCTGATTCCCGCAAGCAGCACAGCACTCCGATGGTGC
GTCTTGGTGGCATCGCCATGGTGCTGGGATTTGGTTTGGCTCTGGCAGTGACATGGGGGCTAGGGGGGTTTGGTTTACTT
GCGCCTGCGAAGGATCAGCTGATCTGGTCCACGCTGGCGGGGGCTCTTTGCTTTTTTGTGATTGGCCTGGCAGATGATTT
GTTCGCTTTGTCTCCTTGGCCTCGTTTGGCAGGACAGGTGGCCGTTGCGTTTGCGATGTGGACTCAGGGGGTACGCATTG
GAACGATTGAGTTCCCCTGGTTGGGGGAAGGCTCTTCTTCTTTAATTGCCTTGCCGGATTTGCTTAGCCTTGCTGCCACG
CTGGTTTGGCTTGTGGGTATTACCAATGCCATCAATTGGCTAGATGGTCTTGATGGTCTTGCGGCAGGAGTGGCTGGTAT
TGCCGCTGTTGGTCTTGTGTCGGTCAGTTTTTCACTTCATCAAGTGGCTGCAGGTTTTCTGGCAGCCGCTTTGGCGGGCT
GTTGTCTTGGTTTTCTGCGCCATAACTTCAATCCAGCCCGCATTTTTATGGGTGATGGTGGCTCCTATTTCCTTGGCTTT
ACCTTGGCAGCAATCAGCATCGTGGGACCAGCCAAGGGACTCACCACCGTCAGTCTGTTGCTGCCACTGTTGATTTTGTC
GCTGCCTTTGGCTGATATGTCGGCAGTGATTATGGGGCGTTTGCGGGAGGGCCGTTCTCCCTTTTATCCTGACCGTCGCC
ATCTTCATCACCGTCTGCTGCGCGCAGGCTTCAGTCATCGCCGAACGGTGTTGTTGATCTACGTGTTTACGCAATGGCTA
GCAGCATTGGCCCTTGTGGTGGCCAATGCTGAAATGCGTTTTTTGTGGTTGGCTTTAGCGACAGCCATTCTCGTGGCGAC
AGTCGTGATCAGCCGTCGTCAACGTAGTGCTGAAGGTGCCTTCGTAGACCCTTCTAGCTCCCTTGCAGCTCCTAGTGATC
CACGTGCCTATCGCGATGGTCATGGCTGA

Upstream 100 bases:

>100_bases
GCGCTTGCTTGACTGGCCCTTCGCTCTGGAATCACAGGTTTGCTCTGCCTAGGATTCATTCCATCGGCCCTTCTTTAGGT
CGCTTGCTACAGGAGCAAAC

Downstream 100 bases:

>100_bases
AGCTTCGGCTAGGCGTGGTGTTGAGATCCTTTGCATTGGCACGGAGCTGTTGCTTGGCAACATCCTTAACAGCAATGCTC
GTTGGTTAGCTGAGGAGCTG

Product: glycosyl transferase

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTLASSPFAFATASFVMAAVLTTLVVPLVRALGLHLGLTDQPDSRKQHSTPMVRLGGIAMVLGFGLALAVTWGLGGFGLL
APAKDQLIWSTLAGALCFFVIGLADDLFALSPWPRLAGQVAVAFAMWTQGVRIGTIEFPWLGEGSSSLIALPDLLSLAAT
LVWLVGITNAINWLDGLDGLAAGVAGIAAVGLVSVSFSLHQVAAGFLAAALAGCCLGFLRHNFNPARIFMGDGGSYFLGF
TLAAISIVGPAKGLTTVSLLLPLLILSLPLADMSAVIMGRLREGRSPFYPDRRHLHHRLLRAGFSHRRTVLLIYVFTQWL
AALALVVANAEMRFLWLALATAILVATVVISRRQRSAEGAFVDPSSSLAAPSDPRAYRDGHG

Sequences:

>Translated_382_residues
MTLASSPFAFATASFVMAAVLTTLVVPLVRALGLHLGLTDQPDSRKQHSTPMVRLGGIAMVLGFGLALAVTWGLGGFGLL
APAKDQLIWSTLAGALCFFVIGLADDLFALSPWPRLAGQVAVAFAMWTQGVRIGTIEFPWLGEGSSSLIALPDLLSLAAT
LVWLVGITNAINWLDGLDGLAAGVAGIAAVGLVSVSFSLHQVAAGFLAAALAGCCLGFLRHNFNPARIFMGDGGSYFLGF
TLAAISIVGPAKGLTTVSLLLPLLILSLPLADMSAVIMGRLREGRSPFYPDRRHLHHRLLRAGFSHRRTVLLIYVFTQWL
AALALVVANAEMRFLWLALATAILVATVVISRRQRSAEGAFVDPSSSLAAPSDPRAYRDGHG
>Mature_381_residues
TLASSPFAFATASFVMAAVLTTLVVPLVRALGLHLGLTDQPDSRKQHSTPMVRLGGIAMVLGFGLALAVTWGLGGFGLLA
PAKDQLIWSTLAGALCFFVIGLADDLFALSPWPRLAGQVAVAFAMWTQGVRIGTIEFPWLGEGSSSLIALPDLLSLAATL
VWLVGITNAINWLDGLDGLAAGVAGIAAVGLVSVSFSLHQVAAGFLAAALAGCCLGFLRHNFNPARIFMGDGGSYFLGFT
LAAISIVGPAKGLTTVSLLLPLLILSLPLADMSAVIMGRLREGRSPFYPDRRHLHHRLLRAGFSHRRTVLLIYVFTQWLA
ALALVVANAEMRFLWLALATAILVATVVISRRQRSAEGAFVDPSSSLAAPSDPRAYRDGHG

Specific function: Catalyzes the formation of undecaprenyl-PP-N- acetylglucosamine. Involved in the synthesis of anionic cell-wall polymers as it mediates the initiation of the linkage unit formation that appears to be common to the two types of teichoic acids attached to t

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790218, Length=290, Percent_Identity=30.6896551724138, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000715
- InterPro:   IPR018480 [H]

Pfam domain/function: PF00953 Glycos_transf_4 [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 40291; Mature: 40160

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLASSPFAFATASFVMAAVLTTLVVPLVRALGLHLGLTDQPDSRKQHSTPMVRLGGIAM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHH
VLGFGLALAVTWGLGGFGLLAPAKDQLIWSTLAGALCFFVIGLADDLFALSPWPRLAGQV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AVAFAMWTQGVRIGTIEFPWLGEGSSSLIALPDLLSLAATLVWLVGITNAINWLDGLDGL
HHHHHHHHCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGVAGIAAVGLVSVSFSLHQVAAGFLAAALAGCCLGFLRHNFNPARIFMGDGGSYFLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
TLAAISIVGPAKGLTTVSLLLPLLILSLPLADMSAVIMGRLREGRSPFYPDRRHLHHRLL
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RAGFSHRRTVLLIYVFTQWLAALALVVANAEMRFLWLALATAILVATVVISRRQRSAEGA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FVDPSSSLAAPSDPRAYRDGHG
EECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLASSPFAFATASFVMAAVLTTLVVPLVRALGLHLGLTDQPDSRKQHSTPMVRLGGIAM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHH
VLGFGLALAVTWGLGGFGLLAPAKDQLIWSTLAGALCFFVIGLADDLFALSPWPRLAGQV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AVAFAMWTQGVRIGTIEFPWLGEGSSSLIALPDLLSLAATLVWLVGITNAINWLDGLDGL
HHHHHHHHCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGVAGIAAVGLVSVSFSLHQVAAGFLAAALAGCCLGFLRHNFNPARIFMGDGGSYFLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
TLAAISIVGPAKGLTTVSLLLPLLILSLPLADMSAVIMGRLREGRSPFYPDRRHLHHRLL
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RAGFSHRRTVLLIYVFTQWLAALALVVANAEMRFLWLALATAILVATVVISRRQRSAEGA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FVDPSSSLAAPSDPRAYRDGHG
EECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12101296; 9384377 [H]