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The map label for this gene is leuD [H]

Identifier: 124024157

GI number: 124024157

Start: 2168799

End: 2169431

Strand: Reverse

Name: leuD [H]

Synonym: P9303_24681

Alternate gene names: 124024157

Gene position: 2169431-2168799 (Counterclockwise)

Preceding gene: 124024158

Following gene: 124024156

Centisome position: 80.87

GC content: 53.4

Gene sequence:

>633_bases
ATGACGATTGAGGCTATACCTTTCCCCCATGGCTCTATCGACCAGGTTCAAGGCCGTGCCTTGATGTTGCGTGGTGACGA
CATTGACACGGATCGGATCATCCCAGCCCGGTTTCTTAAGTGTGTGAGTTTCGAGGCGCTGGGAGATCAGGTTTTTGAGG
ATGATCGCTTGGAACTGGGTGGTTCACATCCCTTCGACCTGAAGGTTCATCAGGGTGCTTCGATCTTGGTTGTTAATGAT
AATTTCGGCTGTGGCTCTAGTCGTGAACATGCACCTCAAGCTCTGATGCGCTGGGGTATTCGTGCGCTGATTGGACAGAG
CTTTGCAGAAATCTTCTATGGCAACTGTCTAGCGCTGGGAATTCCATGTGCAACTGCCAGCCTTGCTCAGATTGATGCCT
TGCAGCAGGCGGTGGCAGCGGATCCTTCGCAGAGCTGGGGCTTCAATCTTCAGGCTCAGCAGTTGACGAGTGCTGCAAGT
TCATGGGATTTAAAGATTGAGTCTGGCCCTCGCGAGATGCTGCTTAGCGGACGTTGGGATGCCACATCGCAGTTGGTGGC
TCAGGACGCAGCCCTAAAGCGCACGATGGCAGACCTCCCATACCTCAATGATTTTGTGGCGCCGCTGCATTGA

Upstream 100 bases:

>100_bases
TGATGAGTCCCGCAATGGTGGCTGCTGCTGCCATTACCGGCCAGGTCACCGATGTGCGCAAGCTAATCAGCAACACTGTT
CCTTCTAAAAGCTTCCACCA

Downstream 100 bases:

>100_bases
AGGTTGTTTGTTGAGGAAACGGAAGATTGAATTTTTAGTGATTCGCTGCGGCAGATCATTGGCAGCGTGTCTATGAAAAG
AGAAAGCGCGCTGGTCATGG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTIEAIPFPHGSIDQVQGRALMLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVND
NFGCGSSREHAPQALMRWGIRALIGQSFAEIFYGNCLALGIPCATASLAQIDALQQAVAADPSQSWGFNLQAQQLTSAAS
SWDLKIESGPREMLLSGRWDATSQLVAQDAALKRTMADLPYLNDFVAPLH

Sequences:

>Translated_210_residues
MTIEAIPFPHGSIDQVQGRALMLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVND
NFGCGSSREHAPQALMRWGIRALIGQSFAEIFYGNCLALGIPCATASLAQIDALQQAVAADPSQSWGFNLQAQQLTSAAS
SWDLKIESGPREMLLSGRWDATSQLVAQDAALKRTMADLPYLNDFVAPLH
>Mature_209_residues
TIEAIPFPHGSIDQVQGRALMLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELGGSHPFDLKVHQGASILVVNDN
FGCGSSREHAPQALMRWGIRALIGQSFAEIFYGNCLALGIPCATASLAQIDALQQAVAADPSQSWGFNLQAQQLTSAASS
WDLKIESGPREMLLSGRWDATSQLVAQDAALKRTMADLPYLNDFVAPLH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=189, Percent_Identity=34.9206349206349, Blast_Score=110, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321429, Length=114, Percent_Identity=39.4736842105263, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22839; Mature: 22708

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEAIPFPHGSIDQVQGRALMLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELG
CCEECCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCC
GSHPFDLKVHQGASILVVNDNFGCGSSREHAPQALMRWGIRALIGQSFAEIFYGNCLALG
CCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IPCATASLAQIDALQQAVAADPSQSWGFNLQAQQLTSAASSWDLKIESGPREMLLSGRWD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHEECCCCC
ATSQLVAQDAALKRTMADLPYLNDFVAPLH
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
TIEAIPFPHGSIDQVQGRALMLRGDDIDTDRIIPARFLKCVSFEALGDQVFEDDRLELG
CEECCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCC
GSHPFDLKVHQGASILVVNDNFGCGSSREHAPQALMRWGIRALIGQSFAEIFYGNCLALG
CCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IPCATASLAQIDALQQAVAADPSQSWGFNLQAQQLTSAASSWDLKIESGPREMLLSGRWD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHEECCCCC
ATSQLVAQDAALKRTMADLPYLNDFVAPLH
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]