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The map label for this gene is leuC

Identifier: 124024158

GI number: 124024158

Start: 2169428

End: 2170864

Strand: Reverse

Name: leuC

Synonym: P9303_24691

Alternate gene names: 124024158

Gene position: 2170864-2169428 (Counterclockwise)

Preceding gene: 124024159

Following gene: 124024157

Centisome position: 80.92

GC content: 55.53

Gene sequence:

>1437_bases
TTGAGCTCCGGCACCCTCTACGACAAGGTGTGGGATCTACATCGGGTGGCTGACCTACCTGGTGGATCCACTCAGTTATT
TGTGGGTTTGCATTTGATCCATGAGGTCACCAGCCCGCAGGCTTTCGCTGCTTTGCAGGACAAGGGTTTGCCGGTGCGGT
GCCCTGAGCGCACCGTGGCGACTGTTGATCACATCGTGCCGACGATCAGTCAGAAGCGTCCGTTTGCTGACCCTTTGGCT
GAGGAGATGCTCAGCACCTTGGAACGAAACTGTGCCAATTACGGGATTGTTCTTTGCGGCCTTGGCAGTGGCCGCCAGGG
CATCGTGCATGTGATAGCTCCTGAGTTGGGGCTGACCCAGCCGGGCATGACTGTGGCTTGTGGTGACTCACACACGTCCA
CCCATGGGGCGTTTGGCGCCATTGCGTTCGGCATCGGCACCAGCCAGGTGCGGGATGTGCTTGCCAGTCAGAGTCTTGCC
ATGAACAAGCTCAAGGTGCGCCGGATCTGGGTCGACGGTCAGCTTGGTTCCGGTGTTCATGCCAAGGATTTGATCCTTCA
TGTGATCCGAAGCCTTGGGGTCAAAGCTGGCGTGGGCTATGCCTATGAGTTCGCCGGACCAGCAATTGATGTGCTCTCCA
TGGAAGAACGCATGACCCTCTGCAATATGGCGATTGAGGGAGGCGCCCGCTGCGGATATGTCAATCCTGATGGGGTCACT
TTTGACTATTTGCAAGGACGGTTTTATGCGCCTACTGGGGAGGCTTGGCATCGGGCAGTGGCCTGGTGGAGCAGCTTGGC
CAGTGATCCCAATGCTGTCTTTGATGATGAGGTGATGTTTGATGCGGCCAGTATTGCTCCCATGGTGACCTGGGGCATCA
CTCCTGGTCAGGGCATTGCTGTAGACGAATCGGTACCGACCATTGATTCGCTTGAGCCAAATGAACGTCCAATCGCCGAG
GAAGCTTGTCGCTATATGGATTTGGAGCCGGGAATGGCGATTGAAGGGGTTCCAGTTGATGTTTGTTTTATTGGCAGTTG
TACCAATGGTCGTCTTAGTGATTTAAAGGCTGCTGCCGCTATTGCCAAGGGCCGCCATGTAGCACAAGGGATCAAGGCCT
TTGTGGTGCCAGGGTCAGAACAAGTCGCCAGGGCTGCGGAAGCAGAGGGGCTCGATGGTGTTTTTCGGAAGGCTGGATTT
GAATGGCGTGAACCAGGCTGTTCCATGTGTCTGGCAATGAATCCTGATCGTCTTGAGGGGCGTCAGATCAGTGCAAGTTC
CAGTAACAGGAATTTCAAGGGGCGTCAGGGTTCGTCCAGAGGCCGCACCTTGTTGATGAGTCCCGCAATGGTGGCTGCTG
CTGCCATTACCGGCCAGGTCACCGATGTGCGCAAGCTAATCAGCAACACTGTTCCTTCTAAAAGCTTCCACCAATGA

Upstream 100 bases:

>100_bases
AGGTTGGCAATTCAGGAGCTGAGTGTGGTTCGCAGTCTCGATCTGCTGCGCAGGTTCCTGCTGGATGGGAGCTAAGGTTT
TCGGCTACTGCCTCTGAACC

Downstream 100 bases:

>100_bases
CGATTGAGGCTATACCTTTCCCCCATGGCTCTATCGACCAGGTTCAAGGCCGTGCCTTGATGTTGCGTGGTGACGACATT
GACACGGATCGGATCATCCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVATVDHIVPTISQKRPFADPLA
EEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLA
MNKLKVRRIWVDGQLGSGVHAKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
FDYLQGRFYAPTGEAWHRAVAWWSSLASDPNAVFDDEVMFDAASIAPMVTWGITPGQGIAVDESVPTIDSLEPNERPIAE
EACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAAIAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGF
EWREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ

Sequences:

>Translated_478_residues
MSSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVATVDHIVPTISQKRPFADPLA
EEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLA
MNKLKVRRIWVDGQLGSGVHAKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
FDYLQGRFYAPTGEAWHRAVAWWSSLASDPNAVFDDEVMFDAASIAPMVTWGITPGQGIAVDESVPTIDSLEPNERPIAE
EACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAAIAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGF
EWREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ
>Mature_477_residues
SSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVATVDHIVPTISQKRPFADPLAE
EMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAM
NKLKVRRIWVDGQLGSGVHAKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVTF
DYLQGRFYAPTGEAWHRAVAWWSSLASDPNAVFDDEVMFDAASIAPMVTWGITPGQGIAVDESVPTIDSLEPNERPIAEE
ACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAAIAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGFE
WREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=397, Percent_Identity=28.9672544080605, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI41352693, Length=414, Percent_Identity=24.6376811594203, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=52.6997840172786, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=27.0718232044199, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=443, Percent_Identity=28.6681715575621, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=443, Percent_Identity=28.6681715575621, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=321, Percent_Identity=29.595015576324, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=26.1728395061728, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=50.8510638297872, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322261, Length=404, Percent_Identity=29.4554455445545, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=397, Percent_Identity=27.9596977329975, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320440, Length=456, Percent_Identity=25.6578947368421, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=29.1139240506329, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281365315, Length=395, Percent_Identity=29.1139240506329, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=395, Percent_Identity=29.1139240506329, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=394, Percent_Identity=27.6649746192893, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645686, Length=489, Percent_Identity=25.3578732106339, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137564, Length=375, Percent_Identity=26.4, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PROM3 (A2CCJ0)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018465.1
- ProteinModelPortal:   A2CCJ0
- SMR:   A2CCJ0
- STRING:   A2CCJ0
- GeneID:   4776020
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_24691
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   TLERNCA
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50953; Mature: 50821

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVA
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHH
TVDHIVPTISQKRPFADPLAEEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAMNKLKVRRIWVDGQLGSGVH
CCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCC
AKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
HHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCE
FDYLQGRFYAPTGEAWHRAVAWWSSLASDPNAVFDDEVMFDAASIAPMVTWGITPGQGIA
EHHHCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCEE
VDESVPTIDSLEPNERPIAEEACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAA
ECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHH
IAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGFEWREPGCSMCLAMNPDRLEG
HHHCHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSGTLYDKVWDLHRVADLPGGSTQLFVGLHLIHEVTSPQAFAALQDKGLPVRCPERTVA
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHH
TVDHIVPTISQKRPFADPLAEEMLSTLERNCANYGIVLCGLGSGRQGIVHVIAPELGLTQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLAMNKLKVRRIWVDGQLGSGVH
CCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCC
AKDLILHVIRSLGVKAGVGYAYEFAGPAIDVLSMEERMTLCNMAIEGGARCGYVNPDGVT
HHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCE
FDYLQGRFYAPTGEAWHRAVAWWSSLASDPNAVFDDEVMFDAASIAPMVTWGITPGQGIA
EHHHCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCEE
VDESVPTIDSLEPNERPIAEEACRYMDLEPGMAIEGVPVDVCFIGSCTNGRLSDLKAAAA
ECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHH
IAKGRHVAQGIKAFVVPGSEQVARAAEAEGLDGVFRKAGFEWREPGCSMCLAMNPDRLEG
HHHCHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSSRGRTLLMSPAMVAAAAITGQVTDVRKLISNTVPSKSFHQ
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA