Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 124024084

GI number: 124024084

Start: 2108216

End: 2109262

Strand: Reverse

Name: pyrB

Synonym: P9303_23931

Alternate gene names: 124024084

Gene position: 2109262-2108216 (Counterclockwise)

Preceding gene: 124024085

Following gene: 124024079

Centisome position: 78.63

GC content: 55.11

Gene sequence:

>1047_bases
ATGAGCGGTTGGTCCCATCGGCATATCCTTGATCTGGCCAGCTTCTCCCTTGAGGATTACAGCTCTGTTCTGGAGCTAGC
GCATCGTTTCCGCTCGATGCCTGTCACGGGTGCGCGCAAGTTGCCGGCGTTGCAGGGGCGTTTGGTGGCAACGTTGTTTT
TTGAGCCCAGTACGCGCACCCGTAGCAGCTTTGAGTTGGCGGCCCGTCGTCTTTCGGCTGATGTGCAGAGTTTCACTCCA
GCTAGTAGTTCACTCAGCAAGGGAGAGACTGTGCTTGATACGGCTCGCACCTATGTGGCGATGGGCGCTGATGTGCTGGT
GGTGCGTCATAGCTCGACCAGTGTTCCCGAACAACTTGCTTGTGCTTTAGATCGTTCAGGCGAACGCACGGCTGTGCTCA
ATGGTGGCGATGGGTTGCATAGCCATCCCAGCCAGGGATTGCTTGATCTCTACACCCTCGCTCACTATTTCGATTCGCAG
AATCCCCTGCCTGAAGCCCTTCAGGGCAAGCGGATTGTGATTGTTGGAGACGTTCTCCATTCACGTGTGGCGCGATCCAA
TCTTTGGGCACTCACTGCCTGCGGTGCAGATGTTGTGTTGTGTGGTCCGCCGAGCCTGGTTCCTCAAGATTTTGTCGCTT
TTGTCGAGGCGCCACCTCCTGGTCAATCGCATGACCCTGTTCAACATCGTGGTTGTGTGGAGGTGGTGCGAACCCTTGAG
GAGGCGTTGCCTGGTGCGGATGCGGTGATGACTTTGAGGTTGCAGAAGGAAAGGATGCATCAGCATTTGCTTACTGATCT
CAACCGCTTTCATCGTGATTATGGGCTCACCCACGAGCGCCTCAAGTTATGCGGGAAGCCTGTACCTCTGCTCCATCCTG
GGCCTGTGAACAGAGGGGTTGAGTTGGGTGGATCATTACTGGATGATCACTCCATCAGTCTTGTCGAAGAGCAGGTGCGC
AATGGCATTCCTATCCGCATGGCCTTGCTTTATTTGATGGCAGCTGTTGAGTCTTCCTCAGATCCTTCCTTAGCAGCCAT
CGGCTGA

Upstream 100 bases:

>100_bases
GTTGGTATCTCAAGAACTGTCGCAGTATTAGTCGCCGTGCCAAGGGAGATCGCACGCCGCTTTTGCAGCAAGCCTGGGCT
CCTGTTGCTAGTGATGGTCA

Downstream 100 bases:

>100_bases
GAAGCTATGCCTGCGATCTGAGTGCTGTAGTACTTCATGGCGGCTTGTAGTGCTGCGTCCGGTGCTGCGATTGAGGGGCC
TAAACCAGGCTGCATTGGGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRTRSSFELAARRLSADVQSFTP
ASSSLSKGETVLDTARTYVAMGADVLVVRHSSTSVPEQLACALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHYFDSQ
NPLPEALQGKRIVIVGDVLHSRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQSHDPVQHRGCVEVVRTLE
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGVELGGSLLDDHSISLVEEQVR
NGIPIRMALLYLMAAVESSSDPSLAAIG

Sequences:

>Translated_348_residues
MSGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRTRSSFELAARRLSADVQSFTP
ASSSLSKGETVLDTARTYVAMGADVLVVRHSSTSVPEQLACALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHYFDSQ
NPLPEALQGKRIVIVGDVLHSRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQSHDPVQHRGCVEVVRTLE
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGVELGGSLLDDHSISLVEEQVR
NGIPIRMALLYLMAAVESSSDPSLAAIG
>Mature_347_residues
SGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRTRSSFELAARRLSADVQSFTPA
SSSLSKGETVLDTARTYVAMGADVLVVRHSSTSVPEQLACALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHYFDSQN
PLPEALQGKRIVIVGDVLHSRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQSHDPVQHRGCVEVVRTLEE
ALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGVELGGSLLDDHSISLVEEQVRN
GIPIRMALLYLMAAVESSSDPSLAAIG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=325, Percent_Identity=33.2307692307692, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI2367364, Length=334, Percent_Identity=29.0419161676647, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790703, Length=302, Percent_Identity=28.476821192053, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786469, Length=253, Percent_Identity=30.0395256916996, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=29.9694189602446, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322331, Length=334, Percent_Identity=28.1437125748503, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24642586, Length=334, Percent_Identity=30.2395209580838, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PROM3 (A2CCB6)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018391.1
- ProteinModelPortal:   A2CCB6
- SMR:   A2CCB6
- STRING:   A2CCB6
- GeneID:   4777290
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_23931
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37788; Mature: 37656

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRT
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEEECCCCCC
RSSFELAARRLSADVQSFTPASSSLSKGETVLDTARTYVAMGADVLVVRHSSTSVPEQLA
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
CALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHYFDSQNPLPEALQGKRIVIVGDVLH
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEHHHHH
SRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQSHDPVQHRGCVEVVRTLE
HHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHH
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGV
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCH
ELGGSLLDDHSISLVEEQVRNGIPIRMALLYLMAAVESSSDPSLAAIG
HHCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
SGWSHRHILDLASFSLEDYSSVLELAHRFRSMPVTGARKLPALQGRLVATLFFEPSTRT
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEEECCCCCC
RSSFELAARRLSADVQSFTPASSSLSKGETVLDTARTYVAMGADVLVVRHSSTSVPEQLA
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
CALDRSGERTAVLNGGDGLHSHPSQGLLDLYTLAHYFDSQNPLPEALQGKRIVIVGDVLH
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEHHHHH
SRVARSNLWALTACGADVVLCGPPSLVPQDFVAFVEAPPPGQSHDPVQHRGCVEVVRTLE
HHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHH
EALPGADAVMTLRLQKERMHQHLLTDLNRFHRDYGLTHERLKLCGKPVPLLHPGPVNRGV
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCH
ELGGSLLDDHSISLVEEQVRNGIPIRMALLYLMAAVESSSDPSLAAIG
HHCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA