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The map label for this gene is rne [H]

Identifier: 124024063

GI number: 124024063

Start: 2089136

End: 2091079

Strand: Reverse

Name: rne [H]

Synonym: P9303_23721

Alternate gene names: 124024063

Gene position: 2091079-2089136 (Counterclockwise)

Preceding gene: 124024064

Following gene: 124024062

Centisome position: 77.95

GC content: 55.25

Gene sequence:

>1944_bases
ATGCCCCAGCAAATTGTCATCTCTGAGCAGCTGCGCATCGCAGCTTTGCTCACAGATGAGCGAGTCGATGAGTTGATCGT
CGCTCAGGGTCGATACCAGATCGGAGATGTCTACCTCGGCACAGTCGAGAACGTTCTCCCGGGAATTGATGCAGCTTTTG
TGAATATTGGGGAAAGTGAGAAAAATGGTTTTATCCATGTCACTGATCTGGGCCCTCTGCGTTTGAAGAAGGGTGCTGCA
GGGATTACCGAGCTGCTGGAGCCCCGTCAGCAGGTGTTGGTACAGGTGATGAAGGAGCCAACAGGGTCTAAGGGGCCAAG
GCTTACTGGCAACCTGGCGCTGCCTGGTCGCTACTTGGTGCTGCAGCCCCATGGCCAGGGGGTGAACATCTCTCGCCGGA
TTAATTCCGAAGGGGAACGCAATCGCTTGCGAGCACTTGGCGTACTAGTGAAGCCGCCTGGTGCTGGCCTGTTGGTTCGC
ACCGAAGCCGATGGCATTAGTGAAGATCTGCTGATCGATGATCTGGAGGTTTTACTCAGGCAATGGGAAGCGATCCAGCA
GGCGGCAGACAATGCTCTACCACCGGTACTGCTCAATCGCGATGAAGATTTCATTCATCGCGTTTTAAGAGATCACGTCG
GTCCAGATCTTGTGAGAGTAGTGGTCAATGATGCCGCTGCCATTGAGCGTGTGAGCAGTTTCCTTGGCCAGGAAGGGCCA
AATGTATTAGTTGAGGCCCATAACGAATCCACCGAACTTCTTGAGCATTACCGAGTTAATGCGGCCATCCGAGATGCTCT
CAAGCCCAGGGTAGATCTGCCATCCGGTGGCTACGTGATCATTGAGCCGACGGAGGCACTCACTGTTATTGATGTCAATT
CAGGCTCGTTCACTCGTTCGGCCAATGCTCGTCAGACGGTGCTCTGGACCAATTGTGAGGCAGCTATTGAGATTGCCCGC
CAGCTGAAGCTTCGCAATATTGGCGGTGTGATCATCATCGACTTCATCGACATGGAGTCGCGCCGTGATCAGTTGCAGTT
GCTGGAGCATTTCACAGCAGCCGTTCGCGATGACGCTGCTCGTCCTCAGATTGCCCAGTTGACTGAACTGGGGCTGGTGG
AACTCACTCGTAAGCGTCAGGGGCAGAACATCTATGAATTGTTTGGCAGGGCTTGTCCTGCCTGTGGTGGCCTTGGCCAT
GTAGCGGTACTACCTGGCAAAGATCTGCTACAGCCACTTGCTATGGCGACTGGATTGGTGAGATCAGCAGCCGCTGCTCG
AGCTGAAGCGCAGGTGCCAGCGGATGGCGGGAATGGTCGCCGTAGGCGTGGTGGGCGCATTCGTACCGGTGCTGTTGAGC
TGCCTGCACATACGCCTCCTTCTGATGTGGCGAATGGAGTTGAGTCAACCACGTCTGTTGAACCTGAGAGACAAGAGCCT
TCGGCCTCGTCTGAGGGTGTTATTCGTCGCCAGGAACCTGAATTGGTTGCTGTTCCAATGGATTCAGGGCAGGAGGAGGT
GTTTGCTTGGCTTGGATTAAACCCTTTGCTTTTGATTGAGCCACCTCCAGAGAATGACAACCTTCTGGTTCGCGTGGTTC
GGCCTGGGGAAGATGCTGAGTCTGTCCTTGAGGAGGCTCGTGAGCAGCTTTCAGCTAGTTCCAATCGGCGTCGGCGTCGG
GGACGAGGTGGTGGTCGAGGGGCCGGCCGTGGTGCTCCCGAAGCCAGTGTCGAAACCCCTTCATCCAATGGCACAGAGGT
CGTTGTCCAGCAAGCCAAGGTTGTCGCAAAGAATGAGCCCCTAAGTGTTCAAATCACTCCTCTTGAGGTTGATCATTCAT
CGAGTAGCGCTTCTGTAGAAACCGTTTCTTCAATTTCTGAGCAGCAGGAGAGTGAACCCGAGGATCCGCGCCGCCGCCGC
CGCCGCTCCTCTGCCGCAGGCTGA

Upstream 100 bases:

>100_bases
TTCAACGGCCTTTGGCTGTTAATTCGTTTTCCATCCGATGCATTGAGCCGGCAGGCTATTGATCGGTTCTGAGTCCAGCA
GGGCTCCCCATTTATTTTCT

Downstream 100 bases:

>100_bases
TATGGACCCTTCGGCGGAATCCATCGCCGGAGTTGATGAGGTGGGCCGGGGCTGCTGGTTTGGTCCTGTCTTTGCCGGAG
CGGTTGTCCTGACTGAAGTT

Product: ribonuclease E/G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MPQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESEKNGFIHVTDLGPLRLKKGAA
GITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLVLQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLVR
TEADGISEDLLIDDLEVLLRQWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRSANARQTVLWTNCEAAIEIAR
QLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAARPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGH
VAVLPGKDLLQPLAMATGLVRSAAAARAEAQVPADGGNGRRRRGGRIRTGAVELPAHTPPSDVANGVESTTSVEPERQEP
SASSEGVIRRQEPELVAVPMDSGQEEVFAWLGLNPLLLIEPPPENDNLLVRVVRPGEDAESVLEEAREQLSASSNRRRRR
GRGGGRGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEPLSVQITPLEVDHSSSSASVETVSSISEQQESEPEDPRRRR
RRSSAAG

Sequences:

>Translated_647_residues
MPQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESEKNGFIHVTDLGPLRLKKGAA
GITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLVLQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLVR
TEADGISEDLLIDDLEVLLRQWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRSANARQTVLWTNCEAAIEIAR
QLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAARPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGH
VAVLPGKDLLQPLAMATGLVRSAAAARAEAQVPADGGNGRRRRGGRIRTGAVELPAHTPPSDVANGVESTTSVEPERQEP
SASSEGVIRRQEPELVAVPMDSGQEEVFAWLGLNPLLLIEPPPENDNLLVRVVRPGEDAESVLEEAREQLSASSNRRRRR
GRGGGRGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEPLSVQITPLEVDHSSSSASVETVSSISEQQESEPEDPRRRR
RRSSAAG
>Mature_646_residues
PQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESEKNGFIHVTDLGPLRLKKGAAG
ITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLVLQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLVRT
EADGISEDLLIDDLEVLLRQWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGPN
VLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRSANARQTVLWTNCEAAIEIARQ
LKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAARPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGHV
AVLPGKDLLQPLAMATGLVRSAAAARAEAQVPADGGNGRRRRGGRIRTGAVELPAHTPPSDVANGVESTTSVEPERQEPS
ASSEGVIRRQEPELVAVPMDSGQEEVFAWLGLNPLLLIEPPPENDNLLVRVVRPGEDAESVLEEAREQLSASSNRRRRRG
RGGGRGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEPLSVQITPLEVDHSSSSASVETVSSISEQQESEPEDPRRRRR
RSSAAG

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=404, Percent_Identity=36.6336633663366, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082235, Length=391, Percent_Identity=37.0843989769821, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 70050; Mature: 69919

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESE
CCCCEEECCCCEEEEHHHHHHHHHHHHHCCCEEECCEEECCHHHHHCCCCEEEEECCCCC
KNGFIHVTDLGPLRLKKGAAGITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLV
CCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLVRTEADGISEDLLIDDLEVLLR
ECCCCCCCEEEECCCCCCHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRS
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEC
ANARQTVLWTNCEAAIEIARQLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAA
CCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
RPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGHVAVLPGKDLLQPLAMATGLV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
RSAAAARAEAQVPADGGNGRRRRGGRIRTGAVELPAHTPPSDVANGVESTTSVEPERQEP
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECEEECCCCCCHHHHHHCCCCCCCCCCCCCCC
SASSEGVIRRQEPELVAVPMDSGQEEVFAWLGLNPLLLIEPPPENDNLLVRVVRPGEDAE
CCCCCCCEEECCCCEEEEECCCCHHHEEEEECCCEEEEEECCCCCCCEEEEEECCCCCHH
SVLEEAREQLSASSNRRRRRGRGGGRGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCCCC
LSVQITPLEVDHSSSSASVETVSSISEQQESEPEDPRRRRRRSSAAG
CEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
PQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESE
CCCEEECCCCEEEEHHHHHHHHHHHHHCCCEEECCEEECCHHHHHCCCCEEEEECCCCC
KNGFIHVTDLGPLRLKKGAAGITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLV
CCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLVRTEADGISEDLLIDDLEVLLR
ECCCCCCCEEEECCCCCCHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRS
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEC
ANARQTVLWTNCEAAIEIARQLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAA
CCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
RPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGHVAVLPGKDLLQPLAMATGLV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
RSAAAARAEAQVPADGGNGRRRRGGRIRTGAVELPAHTPPSDVANGVESTTSVEPERQEP
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECEEECCCCCCHHHHHHCCCCCCCCCCCCCCC
SASSEGVIRRQEPELVAVPMDSGQEEVFAWLGLNPLLLIEPPPENDNLLVRVVRPGEDAE
CCCCCCCEEECCCCEEEEECCCCHHHEEEEECCCEEEEEECCCCCCCEEEEEECCCCCHH
SVLEEAREQLSASSNRRRRRGRGGGRGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCCCC
LSVQITPLEVDHSSSSASVETVSSISEQQESEPEDPRRRRRRSSAAG
CEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]