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The map label for this gene is rnhB [H]

Identifier: 124024062

GI number: 124024062

Start: 2088532

End: 2089134

Strand: Reverse

Name: rnhB [H]

Synonym: P9303_23711

Alternate gene names: 124024062

Gene position: 2089134-2088532 (Counterclockwise)

Preceding gene: 124024063

Following gene: 124024060

Centisome position: 77.88

GC content: 59.04

Gene sequence:

>603_bases
ATGGACCCTTCGGCGGAATCCATCGCCGGAGTTGATGAGGTGGGCCGGGGCTGCTGGTTTGGTCCTGTCTTTGCCGGAGC
GGTTGTCCTGACTGAAGTTGCGGCAGTCGAATTGTTGGCTGAGGGGTTAACAGACAGCAAAGCACTGACTGTTCGCCGCA
GGGCTCGCCTAGTGCCGCTGATTGAGGATGCCGCGACGGCTTGGGCTCTTGGACAGGCCTCGGCTCGCGAAATTGACGCG
CTAGGCATTCGCTCAGCGACGGAACTTTCCATGCTGAGGGCACTGCAGCGGCTACCTACACCGTTGGAGCTTGTCTTGGT
TGATGGCGTCTTGCCATTGCGCCTCTGGATGGGGCCACAACGTACGGTGGTGCGCGGCGATAGCAAATGTGCGGCCATCG
CTGCAGCCAGTGTGTTGGCCAAGCAGGCTCGTGATGGGCTGATCAAGCGGTTGGCTAGTCGCTTCTATGGCTACGGTTTG
GAGCGACATGTTGGCTATGGAACGGCGATCCATCGGCGTGCCTTGTTGGATTTAGGCCCTACAGCGCTTCATCGTCGCTC
ATTCCTCACCAAGCTGTTTGCTGTTCATGGCAGCCTCACTTGA

Upstream 100 bases:

>100_bases
AGCGCTTCTGTAGAAACCGTTTCTTCAATTTCTGAGCAGCAGGAGAGTGAACCCGAGGATCCGCGCCGCCGCCGCCGCCG
CTCCTCTGCCGCAGGCTGAT

Downstream 100 bases:

>100_bases
GCTTATGGGTGTCTCTTTGCACCATTGGCTGAAATCCTTGACAAGTTGTTGGCCAACACGGGTTTTGATGCCAAGCAGGA
TGCCGCTGAGAATTGATTGA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MDPSAESIAGVDEVGRGCWFGPVFAGAVVLTEVAAVELLAEGLTDSKALTVRRRARLVPLIEDAATAWALGQASAREIDA
LGIRSATELSMLRALQRLPTPLELVLVDGVLPLRLWMGPQRTVVRGDSKCAAIAAASVLAKQARDGLIKRLASRFYGYGL
ERHVGYGTAIHRRALLDLGPTALHRRSFLTKLFAVHGSLT

Sequences:

>Translated_200_residues
MDPSAESIAGVDEVGRGCWFGPVFAGAVVLTEVAAVELLAEGLTDSKALTVRRRARLVPLIEDAATAWALGQASAREIDA
LGIRSATELSMLRALQRLPTPLELVLVDGVLPLRLWMGPQRTVVRGDSKCAAIAAASVLAKQARDGLIKRLASRFYGYGL
ERHVGYGTAIHRRALLDLGPTALHRRSFLTKLFAVHGSLT
>Mature_200_residues
MDPSAESIAGVDEVGRGCWFGPVFAGAVVLTEVAAVELLAEGLTDSKALTVRRRARLVPLIEDAATAWALGQASAREIDA
LGIRSATELSMLRALQRLPTPLELVLVDGVLPLRLWMGPQRTVVRGDSKCAAIAAASVLAKQARDGLIKRLASRFYGYGL
ERHVGYGTAIHRRALLDLGPTALHRRSFLTKLFAVHGSLT

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=186, Percent_Identity=44.0860215053763, Blast_Score=130, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 21433; Mature: 21433

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSAESIAGVDEVGRGCWFGPVFAGAVVLTEVAAVELLAEGLTDSKALTVRRRARLVPL
CCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHH
IEDAATAWALGQASAREIDALGIRSATELSMLRALQRLPTPLELVLVDGVLPLRLWMGPQ
HHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCC
RTVVRGDSKCAAIAAASVLAKQARDGLIKRLASRFYGYGLERHVGYGTAIHRRALLDLGP
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCH
TALHRRSFLTKLFAVHGSLT
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDPSAESIAGVDEVGRGCWFGPVFAGAVVLTEVAAVELLAEGLTDSKALTVRRRARLVPL
CCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHH
IEDAATAWALGQASAREIDALGIRSATELSMLRALQRLPTPLELVLVDGVLPLRLWMGPQ
HHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCC
RTVVRGDSKCAAIAAASVLAKQARDGLIKRLASRFYGYGLERHVGYGTAIHRRALLDLGP
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCH
TALHRRSFLTKLFAVHGSLT
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]