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The map label for this gene is hemK [C]

Identifier: 124023898

GI number: 124023898

Start: 1959454

End: 1960374

Strand: Reverse

Name: hemK [C]

Synonym: P9303_22051

Alternate gene names: 124023898

Gene position: 1960374-1959454 (Counterclockwise)

Preceding gene: 124023899

Following gene: 124023897

Centisome position: 73.08

GC content: 54.51

Gene sequence:

>921_bases
ATGGCATTGCTGGAGATTTGCGCAGCAGAGCTATTGGCCTGGCGTCGTTTGCAGTTGGCTGAAGGAGGCCGTGCTGTTGA
TTTTGACTGGTTGCTTGATTTGGGTGGAGGTTTGCGCTGGAGCGATCTTCAGCAGCTTTATCTCGATCCACGACGTTCAG
TGCTGCTTGAGCGATCCCTTGATCAGCTGGCAATGATCTGGAAGCAACATCTTGATCATCACATTCCTCTTCAGCATTTG
ATCGGTTGCTGCCCTTGGAGGGACGTTGAGCTGGAGGTCAGTGCGGCGGCATTGATTCCCCGTCAGGAGACTGAGCTGTT
GGTGGATTTTGCTTTGCAAGCATTTGCTCGGAAACCTTTTGGTTGCTGGGCCGATTTGGGGACAGGTTCAGGAGCACTAG
CTGTGGCATTGGCTCGCGCGCTCCCTGTTTGGCGTGGACATGCAGTGGATTGCAGCATCGAGGCCTTGGCTTTGGCGAAA
CGGAACTTGCAAAGGCTTGCGCCCCATGCCTTATGGCAGCTGCATCAGGGCAGTTGGTGGGAGCCATTGCGGCCTTGGTG
GGGAGAGTTCAGCTTGGTGCTGGTCAATCCTCCCTACATCCCAGAAGCTGTAATGGCTCAACTTGAACCTGTGGTCCGTG
ATCATGAGCCGCATTTGGCTCTATGCGGGGGAGCTGATGGGTTGGTGGCCACACGTCAAATCATTGTTGGTGCCATGCAG
GCCTTAGAGCCTGGTGGTTGGCTGTTCTTGGAACACCACCATGACCAGAGCGATGCCGTGCTGGCTTTGATGCGTCAGCA
GGGTTTGGAGAATGTGGAGTACAAGTCAGATTTACTGGGGGTAAGGCGCTTTGCCATTGCTCGGCATCCTGAACATCACG
ACTCTTTGAACCATGGCACTTCTGCCTTCGGCTGTTCTTGA

Upstream 100 bases:

>100_bases
GTCTTCGGCGCGCTTGACAGAACAATTGCTTCAGTTGGAGTTGAAGGGTGTTGTGGTGGCAGAGCCTGGTTTGCATTGGC
GTTTGGCCTAGCCTGTTTCG

Downstream 100 bases:

>100_bases
CGCCAGTGCCTTGGCATCCAGATTGCATGCTGGTTCAGCGGCATTGTTGCCCACAGACACCTTGCCAGCTTTGGCGGCCG
CTCCTGTTCATGCTGCACAG

Product: protein methyltransferase

Products: Protoporphyrin IX; H2O

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MALLEICAAELLAWRRLQLAEGGRAVDFDWLLDLGGGLRWSDLQQLYLDPRRSVLLERSLDQLAMIWKQHLDHHIPLQHL
IGCCPWRDVELEVSAAALIPRQETELLVDFALQAFARKPFGCWADLGTGSGALAVALARALPVWRGHAVDCSIEALALAK
RNLQRLAPHALWQLHQGSWWEPLRPWWGEFSLVLVNPPYIPEAVMAQLEPVVRDHEPHLALCGGADGLVATRQIIVGAMQ
ALEPGGWLFLEHHHDQSDAVLALMRQQGLENVEYKSDLLGVRRFAIARHPEHHDSLNHGTSAFGCS

Sequences:

>Translated_306_residues
MALLEICAAELLAWRRLQLAEGGRAVDFDWLLDLGGGLRWSDLQQLYLDPRRSVLLERSLDQLAMIWKQHLDHHIPLQHL
IGCCPWRDVELEVSAAALIPRQETELLVDFALQAFARKPFGCWADLGTGSGALAVALARALPVWRGHAVDCSIEALALAK
RNLQRLAPHALWQLHQGSWWEPLRPWWGEFSLVLVNPPYIPEAVMAQLEPVVRDHEPHLALCGGADGLVATRQIIVGAMQ
ALEPGGWLFLEHHHDQSDAVLALMRQQGLENVEYKSDLLGVRRFAIARHPEHHDSLNHGTSAFGCS
>Mature_305_residues
ALLEICAAELLAWRRLQLAEGGRAVDFDWLLDLGGGLRWSDLQQLYLDPRRSVLLERSLDQLAMIWKQHLDHHIPLQHLI
GCCPWRDVELEVSAAALIPRQETELLVDFALQAFARKPFGCWADLGTGSGALAVALARALPVWRGHAVDCSIEALALAKR
NLQRLAPHALWQLHQGSWWEPLRPWWGEFSLVLVNPPYIPEAVMAQLEPVVRDHEPHLALCGGADGLVATRQIIVGAMQA
LEPGGWLFLEHHHDQSDAVLALMRQQGLENVEYKSDLLGVRRFAIARHPEHHDSLNHGTSAFGCS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=227, Percent_Identity=30.3964757709251, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787463, Length=214, Percent_Identity=36.9158878504673, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082085, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24582226, Length=240, Percent_Identity=29.1666666666667, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 1.3.3.4

Molecular weight: Translated: 34235; Mature: 34104

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEICAAELLAWRRLQLAEGGRAVDFDWLLDLGGGLRWSDLQQLYLDPRRSVLLERSL
CHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHH
DQLAMIWKQHLDHHIPLQHLIGCCPWRDVELEVSAAALIPRQETELLVDFALQAFARKPF
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCC
GCWADLGTGSGALAVALARALPVWRGHAVDCSIEALALAKRNLQRLAPHALWQLHQGSWW
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPLRPWWGEFSLVLVNPPYIPEAVMAQLEPVVRDHEPHLALCGGADGLVATRQIIVGAMQ
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHH
ALEPGGWLFLEHHHDQSDAVLALMRQQGLENVEYKSDLLGVRRFAIARHPEHHDSLNHGT
HCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCC
SAFGCS
CCCCCC
>Mature Secondary Structure 
ALLEICAAELLAWRRLQLAEGGRAVDFDWLLDLGGGLRWSDLQQLYLDPRRSVLLERSL
HHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHH
DQLAMIWKQHLDHHIPLQHLIGCCPWRDVELEVSAAALIPRQETELLVDFALQAFARKPF
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCC
GCWADLGTGSGALAVALARALPVWRGHAVDCSIEALALAKRNLQRLAPHALWQLHQGSWW
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPLRPWWGEFSLVLVNPPYIPEAVMAQLEPVVRDHEPHLALCGGADGLVATRQIIVGAMQ
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHH
ALEPGGWLFLEHHHDQSDAVLALMRQQGLENVEYKSDLLGVRRFAIARHPEHHDSLNHGT
HCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCC
SAFGCS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protoporphyrinogen IX; O2

Specific reaction: protoporphyrinogen-IX + 1.5 O2 = protoporphyrin-IX + 3 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]