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The map label for this gene is dus1 [H]

Identifier: 124023850

GI number: 124023850

Start: 1916505

End: 1917515

Strand: Direct

Name: dus1 [H]

Synonym: P9303_21571

Alternate gene names: 124023850

Gene position: 1916505-1917515 (Clockwise)

Preceding gene: 124023848

Following gene: 124023853

Centisome position: 71.44

GC content: 58.65

Gene sequence:

>1011_bases
GTGCCTGAACTCAGCCTGCCCGGCAGGGGAACAACAAGGGCACTGCGCTGCAGAGTGCTGCAATCGCCCCTTGCAGGCGT
GAGTGATCAGATCTTCCGAAGCCTGGTTCGGCGCTGGGCACCAGATGCATTGTTATTCACGGAAATGGTCAATGCCACCA
GCCTGGAGCTGGGCCACGGCCTACAGAAAATCGACGAACTCGCCAATGAAGCCGGGCCCATTGGCGTGCAATTGTTCGAT
CACCGCCCAGAAGCGATGGCCGATGCTGCACAGCGAGCAGAGGCTGCTGGTGCCTTCCTGATCGACATCAACATGGGCTG
TCCTGTGCGCAAGATTGCACGCAAGGGCGGTGGCTCTGGTCTGATCCGTGACCCACAACTAGCGGCAAAAATCGTGAGCA
CTGTTGCTGCAGCAGTCAAAATCCCAGTCACCGTGAAGACAAGGCTGGGCTGGTGTGGCAGCGATGCAAGCCCTGTGCGC
TGGTGTCAATGGCTCGAGCAGGCCGGCGCCCAGATGTTGACATTGCATGCTCGAACTCGAGAGCAAGGCTTCAAAGGCTC
AGCTGATTGGCTCGCCATCGCTGCCGTCAAAAGCGCACTGCAAATCCCCGTGATTGCCAATGGCGATGTCAAGAGCGACA
TGGATGCCAAGCGCTGCCTGGCGATCACTGGAGCCGATGGCGTGATGGTGGGCAGAGGCTCGATGGGAGCGCCATGGCTT
GTGGGTCAAATCGATGCAGCACTATCCGGCATACCCGTGCCTGCAACGCCTGGAGCGGCAGAGCGACTCACCATTGCTCG
TGAACAACTTGAAGCGCTGGTACAAGCAAAGGGGGAACATGGACTCTTGATTGCCCGTAAACACATGGGATGGACTTGTA
GCGGCTTCGTCGGCGCGTCGAAACTGCGCCATGCCCTGATGCGTGCACCAACGCCAACGGATGCCATATCACTGTTAGAG
CAAGCCAGCGCAGAACTCATAACGGCATGGCCTGAAGCGACCAACGCCTAA

Upstream 100 bases:

>100_bases
TACGAAAACCAAAAATTCTTATTTTTAGAGAACGCTCCCTTGAATGAAGAGAGGCCATTCTCTCTAGGATATTGATCCGA
TCACTCTGATCATCAACATT

Downstream 100 bases:

>100_bases
TCCAGCCTCAAGTTGCGATCAATCCGCTACCAAGCCACATCCAATAACCCAGCCAATATCACTAATCAATCGCCGTCATC
AACTCAACAGCTCATCATCC

Product: nitrogen regulation protein NifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHGLQKIDELANEAGPIGVQLFD
HRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSGLIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDASPVR
WCQWLEQAGAQMLTLHARTREQGFKGSADWLAIAAVKSALQIPVIANGDVKSDMDAKRCLAITGADGVMVGRGSMGAPWL
VGQIDAALSGIPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFVGASKLRHALMRAPTPTDAISLLE
QASAELITAWPEATNA

Sequences:

>Translated_336_residues
MPELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHGLQKIDELANEAGPIGVQLFD
HRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSGLIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDASPVR
WCQWLEQAGAQMLTLHARTREQGFKGSADWLAIAAVKSALQIPVIANGDVKSDMDAKRCLAITGADGVMVGRGSMGAPWL
VGQIDAALSGIPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFVGASKLRHALMRAPTPTDAISLLE
QASAELITAWPEATNA
>Mature_335_residues
PELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHGLQKIDELANEAGPIGVQLFDH
RPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSGLIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDASPVRW
CQWLEQAGAQMLTLHARTREQGFKGSADWLAIAAVKSALQIPVIANGDVKSDMDAKRCLAITGADGVMVGRGSMGAPWLV
GQIDAALSGIPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFVGASKLRHALMRAPTPTDAISLLEQ
ASAELITAWPEATNA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=265, Percent_Identity=33.5849056603774, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI239788483, Length=242, Percent_Identity=31.404958677686, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=31.3559322033898, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI31742496, Length=221, Percent_Identity=33.9366515837104, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI8923374, Length=142, Percent_Identity=33.0985915492958, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789660, Length=311, Percent_Identity=35.6913183279743, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1788462, Length=264, Percent_Identity=34.469696969697, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693211, Length=323, Percent_Identity=27.8637770897833, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=254, Percent_Identity=29.5275590551181, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543114, Length=244, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=31.0185185185185, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=31.6239316239316, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.2035398230088, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=224, Percent_Identity=26.3392857142857, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24580595, Length=255, Percent_Identity=32.5490196078431, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920448, Length=255, Percent_Identity=32.5490196078431, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=32.6271186440678, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19921524, Length=146, Percent_Identity=39.7260273972603, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45549423, Length=154, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35508; Mature: 35376

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHG
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
LQKIDELANEAGPIGVQLFDHRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSG
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCC
LIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDASPVRWCQWLEQAGAQMLTLHARTR
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHH
EQGFKGSADWLAIAAVKSALQIPVIANGDVKSDMDAKRCLAITGADGVMVGRGSMGAPWL
HCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCH
VGQIDAALSGIPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFVGAS
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCHHHHH
KLRHALMRAPTPTDAISLLEQASAELITAWPEATNA
HHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
PELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHG
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
LQKIDELANEAGPIGVQLFDHRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSG
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCC
LIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDASPVRWCQWLEQAGAQMLTLHARTR
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHH
EQGFKGSADWLAIAAVKSALQIPVIANGDVKSDMDAKRCLAITGADGVMVGRGSMGAPWL
HCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCH
VGQIDAALSGIPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFVGAS
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCHHHHH
KLRHALMRAPTPTDAISLLEQASAELITAWPEATNA
HHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]