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The map label for this gene is pdhC [H]

Identifier: 124023822

GI number: 124023822

Start: 1893868

End: 1895187

Strand: Reverse

Name: pdhC [H]

Synonym: P9303_21291

Alternate gene names: 124023822

Gene position: 1895187-1893868 (Counterclockwise)

Preceding gene: 124023823

Following gene: 124023817

Centisome position: 70.65

GC content: 56.89

Gene sequence:

>1320_bases
ATGCCTGCTCTGAGCTCAACGATGACGGAGGGCAAGATCGTTGAGTGGCTTAAGCAACCTGGCGACAAGGTTGGGCGTGG
TGAGTCGGTGCTTGTGGTGGAGTCAGATAAAGCGGATATGGATGTGGAGTCATTTCAAGATGGCTACTTGGCCGCGGTCT
TGATGCCTGCTGGTCGTTCGGCTCCAGTGGGTGAAACGATTGGTTTGATCGTTGAAAGTGAGGCCGAAATCGCGGCTGTT
CAGGCCAATGCCCCTGCTGCGCCAGCGTCTGATCCTGCCCCTCTCAAGGCCGCTGCAAAAGTTGTCGATGACCATGCCCC
AGCATCTACTCCGGCGCCCGTCGTGGAGAGTCCCCCTGTTGCTGCGCCGCCGCCTGTTACCAGCCAAGCAGTAGAGAGTG
ACAAACGCATCGTTGCTTCCCCGCGGGCTAAAAAACTTGCTGCGCAGATGGGTGTTGATCTGGCCAAGTTGAGAGGTAGC
GGACCCCATGGCCGTATCCAGGCTGAAGACGTGCAGCTGGCTGCAGGTCAGCCGATCAGTGTGCCTCAGGTTGCTGAAGG
AAACGCTTCTTTCGCAACGACGCATGCAACTTCTGCAGGCGTTGCTCATGCAGTGTCATCTCCTGTAGGTCAGAGCTTTG
GGGCCCCGGGAGAAACCGCAGCCTTCAACAACCTCCAACAAGCGGTCAACCGCAATATGGAGGCCAGTTTGGCCTTCCCC
TGCTTCAGGGTTGGCTACACGATCACGACTGATCAGTTGGATGCTTTTTACAAGCAGGTGAAGCCTAAGGGCGTCACGAT
GACAGCCCTTCTGGCCAAAGCCGTGGCCTTGACGCTTGTGCGTCATCCCCAGGTGAATGCTGCCTACAGCACTGCTGGGA
TGGTTTATCCAGAGCAGGTGAATGTTGCTATTGCAGTGGCGATGGACGATGGCGGTCTGATTACACCGGTTTTGCAGAAT
GCTGATCGCACTGATCTCTATGAGATGTCGCGGCAGTGGGCCGATCTTGTGAAGCGTTCACGCAGCAAGCAGCTGCAACC
CGAGGAATACAGCACTGGTACTTTCACACTCTCCAATCTGGGCATGTTTGGTGTGGATCGCTTTGATGCAATCTTGCCCC
CTGGCACTGGCGCAATTTTGGCGGTAGCTGCATCGCGGCCTGCTGTGGTGGCAGGAAAGGATGGCTCGATTGGGGTCAAG
CGCCAGATGCAGGTGAACCTCACTGCCGACCATCGCGTGATTTATGGCGCCGATGGGGCGGCCTTCCTTAAGGACCTGGC
AGAGCTGATTGAGACGCGGGTAGAGAGTTTGGCGCTCTGA

Upstream 100 bases:

>100_bases
AGCCAACTCCTGATATTTGCCTTAGGAACGTTGCAACGAGGTTTGAGCGCCCCTCAAAATTCAAGTCGAGGACAGGACTA
TCGCAACTCACGACATTTTC

Downstream 100 bases:

>100_bases
AAAGATTTCATCAAAAAGAACTGAGTGATAGACATAAAACTCACATCCAACCAGGCCTTAATCTCAATGGAATTGAGGTA
TTTCTTGTAGCTTCTGCGAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MPALSSTMTEGKIVEWLKQPGDKVGRGESVLVVESDKADMDVESFQDGYLAAVLMPAGRSAPVGETIGLIVESEAEIAAV
QANAPAAPASDPAPLKAAAKVVDDHAPASTPAPVVESPPVAAPPPVTSQAVESDKRIVASPRAKKLAAQMGVDLAKLRGS
GPHGRIQAEDVQLAAGQPISVPQVAEGNASFATTHATSAGVAHAVSSPVGQSFGAPGETAAFNNLQQAVNRNMEASLAFP
CFRVGYTITTDQLDAFYKQVKPKGVTMTALLAKAVALTLVRHPQVNAAYSTAGMVYPEQVNVAIAVAMDDGGLITPVLQN
ADRTDLYEMSRQWADLVKRSRSKQLQPEEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAVAASRPAVVAGKDGSIGVK
RQMQVNLTADHRVIYGADGAAFLKDLAELIETRVESLAL

Sequences:

>Translated_439_residues
MPALSSTMTEGKIVEWLKQPGDKVGRGESVLVVESDKADMDVESFQDGYLAAVLMPAGRSAPVGETIGLIVESEAEIAAV
QANAPAAPASDPAPLKAAAKVVDDHAPASTPAPVVESPPVAAPPPVTSQAVESDKRIVASPRAKKLAAQMGVDLAKLRGS
GPHGRIQAEDVQLAAGQPISVPQVAEGNASFATTHATSAGVAHAVSSPVGQSFGAPGETAAFNNLQQAVNRNMEASLAFP
CFRVGYTITTDQLDAFYKQVKPKGVTMTALLAKAVALTLVRHPQVNAAYSTAGMVYPEQVNVAIAVAMDDGGLITPVLQN
ADRTDLYEMSRQWADLVKRSRSKQLQPEEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAVAASRPAVVAGKDGSIGVK
RQMQVNLTADHRVIYGADGAAFLKDLAELIETRVESLAL
>Mature_438_residues
PALSSTMTEGKIVEWLKQPGDKVGRGESVLVVESDKADMDVESFQDGYLAAVLMPAGRSAPVGETIGLIVESEAEIAAVQ
ANAPAAPASDPAPLKAAAKVVDDHAPASTPAPVVESPPVAAPPPVTSQAVESDKRIVASPRAKKLAAQMGVDLAKLRGSG
PHGRIQAEDVQLAAGQPISVPQVAEGNASFATTHATSAGVAHAVSSPVGQSFGAPGETAAFNNLQQAVNRNMEASLAFPC
FRVGYTITTDQLDAFYKQVKPKGVTMTALLAKAVALTLVRHPQVNAAYSTAGMVYPEQVNVAIAVAMDDGGLITPVLQNA
DRTDLYEMSRQWADLVKRSRSKQLQPEEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAVAASRPAVVAGKDGSIGVKR
QMQVNLTADHRVIYGADGAAFLKDLAELIETRVESLAL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=450, Percent_Identity=30.8888888888889, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI203098753, Length=462, Percent_Identity=29.2207792207792, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=27.8260869565217, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=25.9345794392523, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19923748, Length=182, Percent_Identity=33.5164835164835, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786946, Length=446, Percent_Identity=25.5605381165919, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560088, Length=457, Percent_Identity=32.1663019693654, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=24.7641509433962, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=26.7573696145125, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538894, Length=302, Percent_Identity=27.8145695364238, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=30.718954248366, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320352, Length=186, Percent_Identity=31.7204301075269, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321632, Length=82, Percent_Identity=39.0243902439024, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI20129315, Length=444, Percent_Identity=31.3063063063063, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24582497, Length=437, Percent_Identity=29.7482837528604, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=25.3424657534247, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24645909, Length=183, Percent_Identity=32.7868852459016, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45709; Mature: 45578

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALSSTMTEGKIVEWLKQPGDKVGRGESVLVVESDKADMDVESFQDGYLAAVLMPAGRS
CCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHCCCEEEEEEECCCCC
APVGETIGLIVESEAEIAAVQANAPAAPASDPAPLKAAAKVVDDHAPASTPAPVVESPPV
CCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
AAPPPVTSQAVESDKRIVASPRAKKLAAQMGVDLAKLRGSGPHGRIQAEDVQLAAGQPIS
CCCCCCCHHHHHCCCCEECCCHHHHHHHHHCCHHHHHCCCCCCCCEEECCEEEECCCCCC
VPQVAEGNASFATTHATSAGVAHAVSSPVGQSFGAPGETAAFNNLQQAVNRNMEASLAFP
CCCCCCCCCCEEEECCCHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCC
CFRVGYTITTDQLDAFYKQVKPKGVTMTALLAKAVALTLVRHPQVNAAYSTAGMVYPEQV
HHHCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCC
NVAIAVAMDDGGLITPVLQNADRTDLYEMSRQWADLVKRSRSKQLQPEEYSTGTFTLSNL
CEEEEEEECCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
GMFGVDRFDAILPPGTGAILAVAASRPAVVAGKDGSIGVKRQMQVNLTADHRVIYGADGA
CCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEEEEECCEEEEECCHH
AFLKDLAELIETRVESLAL
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PALSSTMTEGKIVEWLKQPGDKVGRGESVLVVESDKADMDVESFQDGYLAAVLMPAGRS
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHCCCEEEEEEECCCCC
APVGETIGLIVESEAEIAAVQANAPAAPASDPAPLKAAAKVVDDHAPASTPAPVVESPPV
CCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
AAPPPVTSQAVESDKRIVASPRAKKLAAQMGVDLAKLRGSGPHGRIQAEDVQLAAGQPIS
CCCCCCCHHHHHCCCCEECCCHHHHHHHHHCCHHHHHCCCCCCCCEEECCEEEECCCCCC
VPQVAEGNASFATTHATSAGVAHAVSSPVGQSFGAPGETAAFNNLQQAVNRNMEASLAFP
CCCCCCCCCCEEEECCCHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCC
CFRVGYTITTDQLDAFYKQVKPKGVTMTALLAKAVALTLVRHPQVNAAYSTAGMVYPEQV
HHHCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCC
NVAIAVAMDDGGLITPVLQNADRTDLYEMSRQWADLVKRSRSKQLQPEEYSTGTFTLSNL
CEEEEEEECCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
GMFGVDRFDAILPPGTGAILAVAASRPAVVAGKDGSIGVKRQMQVNLTADHRVIYGADGA
CCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEEEEECCEEEEECCHH
AFLKDLAELIETRVESLAL
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA