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The map label for this gene is trmH [H]

Identifier: 124023764

GI number: 124023764

Start: 1822430

End: 1823119

Strand: Direct

Name: trmH [H]

Synonym: P9303_20701

Alternate gene names: 124023764

Gene position: 1822430-1823119 (Clockwise)

Preceding gene: 124023761

Following gene: 124023768

Centisome position: 67.93

GC content: 55.22

Gene sequence:

>690_bases
ATGCCTCTGCTGCCTCGCCGATTTGATCGTCTCAAGGCTGTTCTGGATTGCCGAATGGCAGATCTAACAGTTCTGCTTGA
GCACGTCGAAAAGCCCCACAACCTCTCAGCCATTCTGCGCAGCTGCGATGCGGTGGGTGTCCTGGAAGCCCATGCAGTGA
ACTATTCAGGACGCCTGCCCACCTTTAACAGTACCGCTCAGGGAAGCCAAAAGTGGATACCGCTCAAAGAGCACAGCAGC
ATCGAAGTTGCCGTCAAGGCCATCAAGGAAAGTGGCTTTCGCCTTTATGGAACCCATCTAGGTGGAAACGCACGCGACTA
CCGAGACTGCGACTTCTGCGAACCTACAGCCTTCGTCCTGGGAGCTGAGAAATGGGGCCTTAGTGAGCTGGCGACGCCAT
TGATGGATGAAGGGATCTTCATCCCAATGTGCGGCATGGTGCAGTCGTTGAATGTTTCGGTAGCAGCAGCAACCTTGCTA
TTTGAGGCCCTACGTCAACGACGGCTGGCTGGTATCGCACCTCAAGCCGGCGAAGGCCTAGAACCAGCCCTTTATCAACA
GCGCCTATTTGAGTGGGCCTATCCAGAAGTTGCAGCATGGTGCCAGCAGGAAGGCAGGTCATATCCGGCCCTTAACGACC
AAGGCGAAATCCAAGGAAGCCTCCCCCGCAACGTGAGATTGCGCTGTTAA

Upstream 100 bases:

>100_bases
GGCACCGATCATCTCGGCAATCTGACCATAGGTCGCTAACTGGCCGAAAGGGATGAGGGACACGGCATCCCATACCCTCA
AGTCAAAAGACGCTTGGCCC

Downstream 100 bases:

>100_bases
GCAACCAGCTAACACTCAAAATGGCATCAGCACATCTTTGCTTGCCTGCGATCGATTGATCCCAAGTTGGTTTTGACGTG
CTGCGGGAAGACGCAAACCA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLPTFNSTAQGSQKWIPLKEHSS
IEVAVKAIKESGFRLYGTHLGGNARDYRDCDFCEPTAFVLGAEKWGLSELATPLMDEGIFIPMCGMVQSLNVSVAAATLL
FEALRQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGRSYPALNDQGEIQGSLPRNVRLRC

Sequences:

>Translated_229_residues
MPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLPTFNSTAQGSQKWIPLKEHSS
IEVAVKAIKESGFRLYGTHLGGNARDYRDCDFCEPTAFVLGAEKWGLSELATPLMDEGIFIPMCGMVQSLNVSVAAATLL
FEALRQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGRSYPALNDQGEIQGSLPRNVRLRC
>Mature_228_residues
PLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLPTFNSTAQGSQKWIPLKEHSSI
EVAVKAIKESGFRLYGTHLGGNARDYRDCDFCEPTAFVLGAEKWGLSELATPLMDEGIFIPMCGMVQSLNVSVAAATLLF
EALRQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGRSYPALNDQGEIQGSLPRNVRLRC

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=215, Percent_Identity=46.5116279069767, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI1790623, Length=149, Percent_Identity=38.255033557047, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25398; Mature: 25267

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHEECCCCCCC
TFNSTAQGSQKWIPLKEHSSIEVAVKAIKESGFRLYGTHLGGNARDYRDCDFCEPTAFVL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
GAEKWGLSELATPLMDEGIFIPMCGMVQSLNVSVAAATLLFEALRQRRLAGIAPQAGEGL
ECCCCCHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPALYQQRLFEWAYPEVAAWCQQEGRSYPALNDQGEIQGSLPRNVRLRC
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
PLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHEECCCCCCC
TFNSTAQGSQKWIPLKEHSSIEVAVKAIKESGFRLYGTHLGGNARDYRDCDFCEPTAFVL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
GAEKWGLSELATPLMDEGIFIPMCGMVQSLNVSVAAATLLFEALRQRRLAGIAPQAGEGL
ECCCCCHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPALYQQRLFEWAYPEVAAWCQQEGRSYPALNDQGEIQGSLPRNVRLRC
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]