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The map label for this gene is hisF2 [H]

Identifier: 124023716

GI number: 124023716

Start: 1775386

End: 1776186

Strand: Direct

Name: hisF2 [H]

Synonym: P9303_20161

Alternate gene names: 124023716

Gene position: 1775386-1776186 (Clockwise)

Preceding gene: 124023715

Following gene: 124023717

Centisome position: 66.18

GC content: 34.21

Gene sequence:

>801_bases
ATGCCTAAAAAACGAATAATATTTACACTTTTGTACGATAGTCAAAGCGAACATTTCTTGTTAAGCAGGAATTTTCGCGT
GCAGGAAGTTGGAGATTATAATTGGTTAAAAGAAAATTATGACTTCACAAATTTATCTTACTACATTGATGAGCTATTGG
TCATAGATGTATCTCGAAAAAAAAGGAATAGCCAACGTTTTTGCTCTATGCTTAAGAAGCTATCATCAGACTGCTTTCTT
CCAATAGGCGCCGGAGGAGGATTGGATTCGCTAGATAAGGCTAAAATGCTTTTTGATTCAGGTGCTGATAAATTAATTTT
AAACACCTCAGTATATGAAAACCCTGAATTAATAAAAGCTTTAAATAAATTTTACGGGCAACAAGCAATTATTGCGTCTG
TAGATATCAAAAAGAATGAATTGGGTATATTTGACATCTATACGCATAACGGTACAAAGAAGGCATCTTCTTTAAAGGAT
TATCAAAACATAATCTGCAAAAATGAATGGATTGGAGAATTATACTTAAACTCAATGGAGAGAGATGGCACAGGCCAAGG
TTATGACTTTAATATGTTGAAGAGTTTTACTAACCTAAATAAAAAACATCCAATAATTCTTGCAGGAGGTGCTGGTAAAA
CAAGCCATATAGTAGAAGGGTTATTAACAGATGGTGTAGATGCGTGTGCGACAGCTCATTTATTCAATTTTATTGGTGAT
AAATTAGCCAAGACAAGGTGCATTGCAAAGGCGAATGGAATATCATTAGGTGAATGGCTCCCTAAAGCTTCTCTAAGTTA
A

Upstream 100 bases:

>100_bases
GGCCTACCATCATAAAAATGTCATGGGAACACAATTTCATCCTGAAAAAAGTCAAACAAACGGACTGGCACTAATCAACA
ATTTCATGAAGATTTAATCA

Downstream 100 bases:

>100_bases
ATTATTCGTACAAACCCTGTCCATTAAAAACTCAAGCCAAAAAGGGAGTTCAAGAAGACTAATTATTAGTAGAGTCTAAC
TAGAATAAGCAATGGAATAG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2 [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPKKRIIFTLLYDSQSEHFLLSRNFRVQEVGDYNWLKENYDFTNLSYYIDELLVIDVSRKKRNSQRFCSMLKKLSSDCFL
PIGAGGGLDSLDKAKMLFDSGADKLILNTSVYENPELIKALNKFYGQQAIIASVDIKKNELGIFDIYTHNGTKKASSLKD
YQNIICKNEWIGELYLNSMERDGTGQGYDFNMLKSFTNLNKKHPIILAGGAGKTSHIVEGLLTDGVDACATAHLFNFIGD
KLAKTRCIAKANGISLGEWLPKASLS

Sequences:

>Translated_266_residues
MPKKRIIFTLLYDSQSEHFLLSRNFRVQEVGDYNWLKENYDFTNLSYYIDELLVIDVSRKKRNSQRFCSMLKKLSSDCFL
PIGAGGGLDSLDKAKMLFDSGADKLILNTSVYENPELIKALNKFYGQQAIIASVDIKKNELGIFDIYTHNGTKKASSLKD
YQNIICKNEWIGELYLNSMERDGTGQGYDFNMLKSFTNLNKKHPIILAGGAGKTSHIVEGLLTDGVDACATAHLFNFIGD
KLAKTRCIAKANGISLGEWLPKASLS
>Mature_265_residues
PKKRIIFTLLYDSQSEHFLLSRNFRVQEVGDYNWLKENYDFTNLSYYIDELLVIDVSRKKRNSQRFCSMLKKLSSDCFLP
IGAGGGLDSLDKAKMLFDSGADKLILNTSVYENPELIKALNKFYGQQAIIASVDIKKNELGIFDIYTHNGTKKASSLKDY
QNIICKNEWIGELYLNSMERDGTGQGYDFNMLKSFTNLNKKHPIILAGGAGKTSHIVEGLLTDGVDACATAHLFNFIGDK
LAKTRCIAKANGISLGEWLPKASLS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=246, Percent_Identity=26.8292682926829, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 29849; Mature: 29717

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKRIIFTLLYDSQSEHFLLSRNFRVQEVGDYNWLKENYDFTNLSYYIDELLVIDVSRK
CCCCEEEEEEEECCCCCEEEEECCCEEEECCCCHHHHCCCCCCHHHHHHHHEEEEECHHH
KRNSQRFCSMLKKLSSDCFLPIGAGGGLDSLDKAKMLFDSGADKLILNTSVYENPELIKA
HCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LNKFYGQQAIIASVDIKKNELGIFDIYTHNGTKKASSLKDYQNIICKNEWIGELYLNSME
HHHHHCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
RDGTGQGYDFNMLKSFTNLNKKHPIILAGGAGKTSHIVEGLLTDGVDACATAHLFNFIGD
CCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
KLAKTRCIAKANGISLGEWLPKASLS
HHHHHHHHHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
PKKRIIFTLLYDSQSEHFLLSRNFRVQEVGDYNWLKENYDFTNLSYYIDELLVIDVSRK
CCCEEEEEEEECCCCCEEEEECCCEEEECCCCHHHHCCCCCCHHHHHHHHEEEEECHHH
KRNSQRFCSMLKKLSSDCFLPIGAGGGLDSLDKAKMLFDSGADKLILNTSVYENPELIKA
HCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LNKFYGQQAIIASVDIKKNELGIFDIYTHNGTKKASSLKDYQNIICKNEWIGELYLNSME
HHHHHCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
RDGTGQGYDFNMLKSFTNLNKKHPIILAGGAGKTSHIVEGLLTDGVDACATAHLFNFIGD
CCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
KLAKTRCIAKANGISLGEWLPKASLS
HHHHHHHHHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]