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The map label for this gene is yafV [C]

Identifier: 124023592

GI number: 124023592

Start: 1654242

End: 1655063

Strand: Direct

Name: yafV [C]

Synonym: P9303_18921

Alternate gene names: 124023592

Gene position: 1654242-1655063 (Clockwise)

Preceding gene: 124023591

Following gene: 124023593

Centisome position: 61.66

GC content: 56.81

Gene sequence:

>822_bases
GTGACCGACTTTCTGGCAGCCGCTTTGCAGCTGACGAGCACCTCGGAACCAGAGCTCAATTTTGCTGCTGCCGAGGAGCA
GATCGAACTGGCTGCCCGCCGTGGGGCGGAATTGGTGGGATTACCTGAAAATTTTGCCTTTATGGGTGATGACGAGCGAC
GGCTGGAGCTGGCACAAGACTTAGCTCAGCAATGCAGCCGCTTTCTGGTCACCATGGCAAGGCGCTACCAGGTGGTGCTT
CTGGGCGGGGGCTTCCCTGTACCTGCAGGAGATAGCAATCACACGGTCAACAGAGCCGAATTGGTCGGTCGTGATGGTCA
GCTGCTAGCGAGATACGACAAGATTCATCTCTTCGATGTGGATCTACCTGAAGGCAACACCTATCAGGAGTCGGCCACGA
CTACGTCTGGTCGTGAACTACCCCCGGTCGTGGATGTGCCAGGACTATGTCGAGTGGGACTATCCATCTGCTACGACGTG
CGCTTCCCTGAGCTCTATCGCCATCTCGTCGGTGCTGGCGCCGAGTTATTGATGATTCCAGCAGCCTTCACTGCTTTCAC
CGGAAAAGATCACTGGCAGGTCTTACTTCAGGCCAGGGCCATCGAGAACACCTCCTATGTGCTTGCACCTGCCCAGACAG
GTCGTCATTACAGCCGTCGACAGAGCCATGGACATGCCATGGTGATTGATCCCTGGGGAACCGTCTTGGCTGATGCAGGG
GTGAGTCAGGGTGCAGCCATTGCTCCTGTGGATAACTCGCACATTGGTCGCATTCGCGCGCAAATGCCAAGCCTCCAACA
CCGGCAGGCAGCACTGTTCTGA

Upstream 100 bases:

>100_bases
GGATCGGCTTTCTGTGGTTCCTGTTCAGGTCAAACCCGGAGTCCTTTGTGCCTCTTGAAACCACACTGCGATCTAAAGTT
TCGCTCTCTGTTTCAACCCT

Downstream 100 bases:

>100_bases
GCCAATGGCCTCGGCTCCGTCCCGCCCTCTCATCCTGCTGGTGGTTGGTGCCTTGCAGATCCCTTTGGTTTTGGCAGCGT
TGCCGGCCAGAGCGGCAAGC

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTDFLAAALQLTSTSEPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQDLAQQCSRFLVTMARRYQVVL
LGGGFPVPAGDSNHTVNRAELVGRDGQLLARYDKIHLFDVDLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDV
RFPELYRHLVGAGAELLMIPAAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQAALF

Sequences:

>Translated_273_residues
MTDFLAAALQLTSTSEPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQDLAQQCSRFLVTMARRYQVVL
LGGGFPVPAGDSNHTVNRAELVGRDGQLLARYDKIHLFDVDLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDV
RFPELYRHLVGAGAELLMIPAAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQAALF
>Mature_272_residues
TDFLAAALQLTSTSEPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQDLAQQCSRFLVTMARRYQVVLL
GGGFPVPAGDSNHTVNRAELVGRDGQLLARYDKIHLFDVDLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDVR
FPELYRHLVGAGAELLMIPAAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAGV
SQGAAIAPVDNSHIGRIRAQMPSLQHRQAALF

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=273, Percent_Identity=40.6593406593407, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI297632348, Length=273, Percent_Identity=40.6593406593407, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI5031947, Length=273, Percent_Identity=40.6593406593407, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI9910460, Length=273, Percent_Identity=35.8974358974359, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=37.8238341968912, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=271, Percent_Identity=42.0664206642066, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6322335, Length=303, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=30.6569343065693, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI17933642, Length=266, Percent_Identity=36.4661654135338, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=30.8550185873606, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29819; Mature: 29688

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLAAALQLTSTSEPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQD
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEECCCCHHHHHHHHH
LAQQCSRFLVTMARRYQVVLLGGGFPVPAGDSNHTVNRAELVGRDGQLLARYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEECEEEEEEE
DLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDVRFPELYRHLVGAGAELLMIP
ECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHCCCCEEEEEC
AAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
HHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEECCCHHHHHHCC
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQAALF
CCCCCEEECCCCCHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TDFLAAALQLTSTSEPELNFAAAEEQIELAARRGAELVGLPENFAFMGDDERRLELAQD
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEECCCCHHHHHHHHH
LAQQCSRFLVTMARRYQVVLLGGGFPVPAGDSNHTVNRAELVGRDGQLLARYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEECEEEEEEE
DLPEGNTYQESATTTSGRELPPVVDVPGLCRVGLSICYDVRFPELYRHLVGAGAELLMIP
ECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHCCCCEEEEEC
AAFTAFTGKDHWQVLLQARAIENTSYVLAPAQTGRHYSRRQSHGHAMVIDPWGTVLADAG
HHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEECCCHHHHHHCC
VSQGAAIAPVDNSHIGRIRAQMPSLQHRQAALF
CCCCCEEECCCCCHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]