Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 124023586

GI number: 124023586

Start: 1646652

End: 1648049

Strand: Direct

Name: murF [H]

Synonym: P9303_18861

Alternate gene names: 124023586

Gene position: 1646652-1648049 (Clockwise)

Preceding gene: 124023585

Following gene: 124023591

Centisome position: 61.38

GC content: 54.36

Gene sequence:

>1398_bases
ATGAGACTGCGTCTAGGGCAGCTGATCGAGCTTTGGGGTGCTCCGATGCATGTTCACGCGTCTGACCTAGATCTTGACCA
AGCTGTTGGTCCTATCTGCACAGACAGTCGTCAGTTGAGCGAAGGCAGCTTCTTTGTGCCGTTGGTGGGGGAGCATTTCA
ATGGTCATGCCTTTCTTGCTCAAGCTCTAAAACTTGAGGCCCAGGCGGCTGTGGTGGCTCGCAAAGATCATCATCCAGTA
CCCGAGGGTCTCCCCCATTGGATTGTGGAAGACACTCTCGAGGCTTATCAGCAGCTGGGGTTGCTGCATCGCCGTCAGCT
GAATGTACCGGTGGTTGCCGTAACAGGTTCTGCAGGAAAAACCACGACTCGGGAGTTGATCCGTTCTGCACTCACACCCC
TTGGTGAGGTCTTGGCAAGTGCAGGCAACAACAACAACGACGTGGGTGTGCCGCTCACCTTGTTGCAGGCGCATTCAGAT
CATGGCGCAGTTGTGGTGGAGATGGGGATGCGTGGAATTGGGGAAATAGAACGGCTTTCCTGTTGCACCCAACCAGACGT
CGCTGTAATCACCAATATCGGCAATGCACACGTCGGTCGTTTAGGTAGTCGTGCGGCGATTGCTTCCGCCAAGTGTGAGA
TCACCACTTGCCTCAAACCCAGAGGAGTGATCGTCATCCCAGCAGGGGATTCGCTACTGGAAGATGCTCTTGAACGGATC
TGGCGGGGGCGGGTGATCCGCGTGGCAGTTCAAGACAATCAACAATTAGAGCCACCAGAGATTGCAGATCTGCATCGAAG
ACCTCTACCCAAGGCTGACCTCGTTGGCTGCGTTGACCTGGCCAAGGGTGAACTATTGTTAGAGGGAAAGGCCTACACCC
TGCCTTTAGAAGGGATGCATAACGCTTGCAATTTGATGCTGGCACTCGCAGTTGCCCGTGAATTAGATGTCTGCAACGAA
TCGCTATCCAATCTAAAAGTAGACGTTCCTGGTGGCCGTAGTCGACGGCTAAAAGTTGGCAACTTCACTGTTCTGGATGA
AACCTACAACGCTTCGCCGGAAGCCGTGCAAGCAGCATTGGAGCTATTAGCAATTCAACCGGGACGTCACTTTGCAGTAC
TCGGAACCATGCTGGAGCTAGGAGACCAGAGCGTGGCTTTACATCGCCAAGTTGCTGAACGGGCGGTACAAATTGGTTTG
GATGGACTCGTCGTCGTTGCGGCAGGCGCAGAGGCTGAGGCCATGGCAACAGCAGCAGAGTCCTTGCCGCATTTGGCCGT
CGTTGCCAACCCAGAGCAGGCTGCTCAGCCATTGCAGGCTTGGCTTGAGGCCGGCGATGTGGTTCTGCTTAAGGCTAGTC
GTGGTGTCGCTCTAGAGAAATTGATCCCTTTGCTCTAA

Upstream 100 bases:

>100_bases
GTTATGACGAACTTCAGCTTCAGGATGAGGATGATCAACTGCTTGCCAGGACAGCAAGAGTAGGTGTGGGCATGATCATT
TTCGATCAGGATTTGGCTTA

Downstream 100 bases:

>100_bases
GAGGGCTAAGCCTCAATCCTCTCGTTTCACTGCCAACCATTTTTAATCAGTTGTTTGGAGCGGCCAAGAGCGAGAGCACC
ATTGGGAACATCTTTAGTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MRLRLGQLIELWGAPMHVHASDLDLDQAVGPICTDSRQLSEGSFFVPLVGEHFNGHAFLAQALKLEAQAAVVARKDHHPV
PEGLPHWIVEDTLEAYQQLGLLHRRQLNVPVVAVTGSAGKTTTRELIRSALTPLGEVLASAGNNNNDVGVPLTLLQAHSD
HGAVVVEMGMRGIGEIERLSCCTQPDVAVITNIGNAHVGRLGSRAAIASAKCEITTCLKPRGVIVIPAGDSLLEDALERI
WRGRVIRVAVQDNQQLEPPEIADLHRRPLPKADLVGCVDLAKGELLLEGKAYTLPLEGMHNACNLMLALAVARELDVCNE
SLSNLKVDVPGGRSRRLKVGNFTVLDETYNASPEAVQAALELLAIQPGRHFAVLGTMLELGDQSVALHRQVAERAVQIGL
DGLVVVAAGAEAEAMATAAESLPHLAVVANPEQAAQPLQAWLEAGDVVLLKASRGVALEKLIPLL

Sequences:

>Translated_465_residues
MRLRLGQLIELWGAPMHVHASDLDLDQAVGPICTDSRQLSEGSFFVPLVGEHFNGHAFLAQALKLEAQAAVVARKDHHPV
PEGLPHWIVEDTLEAYQQLGLLHRRQLNVPVVAVTGSAGKTTTRELIRSALTPLGEVLASAGNNNNDVGVPLTLLQAHSD
HGAVVVEMGMRGIGEIERLSCCTQPDVAVITNIGNAHVGRLGSRAAIASAKCEITTCLKPRGVIVIPAGDSLLEDALERI
WRGRVIRVAVQDNQQLEPPEIADLHRRPLPKADLVGCVDLAKGELLLEGKAYTLPLEGMHNACNLMLALAVARELDVCNE
SLSNLKVDVPGGRSRRLKVGNFTVLDETYNASPEAVQAALELLAIQPGRHFAVLGTMLELGDQSVALHRQVAERAVQIGL
DGLVVVAAGAEAEAMATAAESLPHLAVVANPEQAAQPLQAWLEAGDVVLLKASRGVALEKLIPLL
>Mature_465_residues
MRLRLGQLIELWGAPMHVHASDLDLDQAVGPICTDSRQLSEGSFFVPLVGEHFNGHAFLAQALKLEAQAAVVARKDHHPV
PEGLPHWIVEDTLEAYQQLGLLHRRQLNVPVVAVTGSAGKTTTRELIRSALTPLGEVLASAGNNNNDVGVPLTLLQAHSD
HGAVVVEMGMRGIGEIERLSCCTQPDVAVITNIGNAHVGRLGSRAAIASAKCEITTCLKPRGVIVIPAGDSLLEDALERI
WRGRVIRVAVQDNQQLEPPEIADLHRRPLPKADLVGCVDLAKGELLLEGKAYTLPLEGMHNACNLMLALAVARELDVCNE
SLSNLKVDVPGGRSRRLKVGNFTVLDETYNASPEAVQAALELLAIQPGRHFAVLGTMLELGDQSVALHRQVAERAVQIGL
DGLVVVAAGAEAEAMATAAESLPHLAVVANPEQAAQPLQAWLEAGDVVLLKASRGVALEKLIPLL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=470, Percent_Identity=32.3404255319149, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49569; Mature: 49569

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRLGQLIELWGAPMHVHASDLDLDQAVGPICTDSRQLSEGSFFVPLVGEHFNGHAFLA
CCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHH
QALKLEAQAAVVARKDHHPVPEGLPHWIVEDTLEAYQQLGLLHRRQLNVPVVAVTGSAGK
HHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
TTTRELIRSALTPLGEVLASAGNNNNDVGVPLTLLQAHSDHGAVVVEMGMRGIGEIERLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHH
CCTQPDVAVITNIGNAHVGRLGSRAAIASAKCEITTCLKPRGVIVIPAGDSLLEDALERI
CCCCCCEEEEEECCCHHHHCCCCCHHHHCCCEEEEEEECCCCEEEEECCHHHHHHHHHHH
WRGRVIRVAVQDNQQLEPPEIADLHRRPLPKADLVGCVDLAKGELLLEGKAYTLPLEGMH
HCCCEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEECCCEEEEEHHHHH
NACNLMLALAVARELDVCNESLSNLKVDVPGGRSRRLKVGNFTVLDETYNASPEAVQAAL
HHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCCHHHHHHHH
ELLAIQPGRHFAVLGTMLELGDQSVALHRQVAERAVQIGLDGLVVVAAGAEAEAMATAAE
HHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
SLPHLAVVANPEQAAQPLQAWLEAGDVVLLKASRGVALEKLIPLL
CCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MRLRLGQLIELWGAPMHVHASDLDLDQAVGPICTDSRQLSEGSFFVPLVGEHFNGHAFLA
CCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHH
QALKLEAQAAVVARKDHHPVPEGLPHWIVEDTLEAYQQLGLLHRRQLNVPVVAVTGSAGK
HHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
TTTRELIRSALTPLGEVLASAGNNNNDVGVPLTLLQAHSDHGAVVVEMGMRGIGEIERLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHH
CCTQPDVAVITNIGNAHVGRLGSRAAIASAKCEITTCLKPRGVIVIPAGDSLLEDALERI
CCCCCCEEEEEECCCHHHHCCCCCHHHHCCCEEEEEEECCCCEEEEECCHHHHHHHHHHH
WRGRVIRVAVQDNQQLEPPEIADLHRRPLPKADLVGCVDLAKGELLLEGKAYTLPLEGMH
HCCCEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEECCCEEEEEHHHHH
NACNLMLALAVARELDVCNESLSNLKVDVPGGRSRRLKVGNFTVLDETYNASPEAVQAAL
HHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCCHHHHHHHH
ELLAIQPGRHFAVLGTMLELGDQSVALHRQVAERAVQIGLDGLVVVAAGAEAEAMATAAE
HHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
SLPHLAVVANPEQAAQPLQAWLEAGDVVLLKASRGVALEKLIPLL
CCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]