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The map label for this gene is thrS

Identifier: 124023562

GI number: 124023562

Start: 1622698

End: 1624620

Strand: Reverse

Name: thrS

Synonym: P9303_18621

Alternate gene names: 124023562

Gene position: 1624620-1622698 (Counterclockwise)

Preceding gene: 124023564

Following gene: 124023561

Centisome position: 60.56

GC content: 43.63

Gene sequence:

>1923_bases
ATGCCTATTATTACACTTCCAGATGGAAATAAAAAGAAGTTTGATCAACCCGTAACCATTATGGAGGTGGCCGAAAGCCT
TGGGCCTGGATTAGCAAAGGCGGCTATTGCAGGACGAGTCAATGGCGTATTGCTTGACACCTGTATCCCTATTGAGAAAG
ACTCTGAAGTCAATATCATCACGGCTAAAGACCAAGATGGAATTGAGACTATTCGCCACTCATTCGCTCACTTGATCGGT
CATGCCGTAAAGCAATTATATCCCGAAGCAAAAATGGCTATTGGTCCAGTTATTGAAGACGGATTTTATTATGATATTGC
TTATGATCAGCCTTTTACGCCTAAAGACTTGGAAGCGATTGAGGCTCGCATGAAAGAGCTGGTTAAACTTGACTACGACG
TCAATGTTGAAATAGTTTCGAGGGAAGAGGCTCATAAGGAATTCGAAAAGCGATGCGAGCCCTACAAGATCGAAATCGTA
GATGAAATTCCTGAGAATGAAATTATTAAGCTATATCGACATCAAGAATATACTGATATGTGTAGAGGTCCACATGTTCC
TAACACAAGGCATTTACGCACTTTCAAATTAATGAAAGTATCAGGTGCTTATTGGCGAGGTGATTCAAATAAAACAATGT
TGCAGCGCATCTATGGCACGGCCTGGGGAAGTTCCAAAGAGCTGAAAGCTTATCTCAAGCGCCTTGAAGAAGCTGAAAAG
CGCGATCATCGCAGGATCGCCAAACAAATGTCTTTATTTCATACTCAAGAAGAAGCTCCTGGGATGATCTTTTGGCATGC
CAAGGGTTGGGCTATTTATCAGGTTTTAGAGCAATATATTCGCGAGACCCTTAGCCTGCATGCTTACCAAGAAATCCGAA
CACCTCAGGTTGTAGACCGCTCCTTATGGGAGAAATCAGGCCATTGGGAGAAGTTCAAAGATGACATGTTCACAACGACA
TCTGAGAATCGGGAATATGCTATCAAGCCGATGAATTGTCCCTGCCATGTACAGATCTTTAATCAAGGCCTAAAAAGTTA
CCGTGACCTGCCAATTAGATTGGCAGAGTTTGGTTCATGCTTAAGGAATGAACCGTCTGGCTCACTTCATGGTCTCATGC
GCGTGCGCAATTTTGTTCAAGACGATGCTCACATCTTTTGCACTGAGCTTCAGGTTCAGGAAGAGGTCTCTAAGTTTATT
GATCTAGTCTTTGAGATTTACAGATCATTTGGGTTTGACTCGGTGCTTATAAAGTTATCAACCAGGCCCGAAAAGCGTGT
TGGTAGTGATGAGATCTGGGACAAATCAGAGAAGGCCTTGTCCGATGCATTGGATGCTAAAGGTCTTGCCTGGGACTTAT
TGCCAGGGGAAGGTGCATTCTACGGACCTAAAATTGAGTTTTCTTTAAAAGACTGTCTTGGTAGAGTTTGGCAATGTGGA
ACGATCCAGGTTGACTTCTCGATGCCGGAGCGCTTGGGAGCATCTTATGTAGCAGAAGACAGTCAGCGCAGAACACCAGT
AATGTTGCATCGAGCAATTCTGGGTTCTTTTGAACGTTTTATCGGAATTCTGATCGAGCACTATGCTGGACGAATGCCTG
TCTGGCTAGCACCTGTGCAGGTGGCAGTGATGGGGATTACAGACCGTAATGCTCAGACTTGTCAGGATGTTTGCAAGAAG
TTATCAGCCCTAGAATATCGAACTGAAGTTGACTTGAGAAACGAAAAAATTGGTTTTAAAGTTCGCGAACATACTCTTCA
GCGTGTACCATTTTTAATCATTATTGGTGATAAAGAACAACAAAGTGGAGAGGTGGCTGTGCGCACTCGAGAGGGTAAGG
ACTTTGGCAGCATGCCTTTGAATAGCTTCATATCACTCCTGGATGAAGCAATTGCTCTTAAAGGTAGATCAGGTGTCTCT
TGA

Upstream 100 bases:

>100_bases
GCAAAATATTTTAGTCCAGCATTCAGAGGTGGAAGGATTGCGAATGATATAGCTGATAAAATCGAACGCTATTGCAACTC
ATCAAAGAAAACTCCTTGCT

Downstream 100 bases:

>100_bases
CACAAGTTATCAAGAGTTAGGTAATAATGACAATCGGAGAATGCCTCGCTTTGGAGGAAAGCTCACTTCCATTTGGATGA
CAAGCTGAATGGCAAATTGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MPIITLPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNIITAKDQDGIETIRHSFAHLIG
HAVKQLYPEAKMAIGPVIEDGFYYDIAYDQPFTPKDLEAIEARMKELVKLDYDVNVEIVSREEAHKEFEKRCEPYKIEIV
DEIPENEIIKLYRHQEYTDMCRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHAYQEIRTPQVVDRSLWEKSGHWEKFKDDMFTTT
SENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSCLRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFI
DLVFEIYRSFGFDSVLIKLSTRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRMPVWLAPVQVAVMGITDRNAQTCQDVCKK
LSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQQSGEVAVRTREGKDFGSMPLNSFISLLDEAIALKGRSGVS

Sequences:

>Translated_640_residues
MPIITLPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNIITAKDQDGIETIRHSFAHLIG
HAVKQLYPEAKMAIGPVIEDGFYYDIAYDQPFTPKDLEAIEARMKELVKLDYDVNVEIVSREEAHKEFEKRCEPYKIEIV
DEIPENEIIKLYRHQEYTDMCRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHAYQEIRTPQVVDRSLWEKSGHWEKFKDDMFTTT
SENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSCLRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFI
DLVFEIYRSFGFDSVLIKLSTRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRMPVWLAPVQVAVMGITDRNAQTCQDVCKK
LSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQQSGEVAVRTREGKDFGSMPLNSFISLLDEAIALKGRSGVS
>Mature_639_residues
PIITLPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNIITAKDQDGIETIRHSFAHLIGH
AVKQLYPEAKMAIGPVIEDGFYYDIAYDQPFTPKDLEAIEARMKELVKLDYDVNVEIVSREEAHKEFEKRCEPYKIEIVD
EIPENEIIKLYRHQEYTDMCRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEKR
DHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHAYQEIRTPQVVDRSLWEKSGHWEKFKDDMFTTTS
ENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSCLRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFID
LVFEIYRSFGFDSVLIKLSTRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCGT
IQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRMPVWLAPVQVAVMGITDRNAQTCQDVCKKL
SALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQQSGEVAVRTREGKDFGSMPLNSFISLLDEAIALKGRSGVS

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=630, Percent_Identity=37.7777777777778, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI38570111, Length=613, Percent_Identity=38.3360522022839, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI20070344, Length=647, Percent_Identity=34.4667697063369, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1788013, Length=632, Percent_Identity=62.1835443037975, Blast_Score=857, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=640, Percent_Identity=38.125, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71984184, Length=640, Percent_Identity=38.125, Blast_Score=459, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322112, Length=628, Percent_Identity=37.1019108280255, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322654, Length=398, Percent_Identity=37.1859296482412, Blast_Score=295, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24583842, Length=641, Percent_Identity=37.7535101404056, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI28574141, Length=641, Percent_Identity=37.7535101404056, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24583839, Length=641, Percent_Identity=37.7535101404056, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_PROM3 (A2CAU4)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017869.1
- ProteinModelPortal:   A2CAU4
- SMR:   A2CAU4
- STRING:   A2CAU4
- GeneID:   4777124
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_18621
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   EVIRHSC
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73292; Mature: 73161

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIITLPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNII
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
TAKDQDGIETIRHSFAHLIGHAVKQLYPEAKMAIGPVIEDGFYYDIAYDQPFTPKDLEAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEECCCEEEEEECCCCCCCHHHHHH
EARMKELVKLDYDVNVEIVSREEAHKEFEKRCEPYKIEIVDEIPENEIIKLYRHQEYTDM
HHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHHH
CRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
HCCCCCCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHAYQEIRTPQVVDR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLWEKSGHWEKFKDDMFTTTSENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSC
HHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHH
LRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFIDLVFEIYRSFGFDSVLIKLS
HCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
CCCCCCCCCCHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRMPVWLAPVQ
EEEEECCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEE
VAVMGITDRNAQTCQDVCKKLSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEECCCCC
QSGEVAVRTREGKDFGSMPLNSFISLLDEAIALKGRSGVS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PIITLPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNII
CEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
TAKDQDGIETIRHSFAHLIGHAVKQLYPEAKMAIGPVIEDGFYYDIAYDQPFTPKDLEAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEECCCEEEEEECCCCCCCHHHHHH
EARMKELVKLDYDVNVEIVSREEAHKEFEKRCEPYKIEIVDEIPENEIIKLYRHQEYTDM
HHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHHH
CRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
HCCCCCCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHAYQEIRTPQVVDR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLWEKSGHWEKFKDDMFTTTSENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSC
HHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHH
LRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFIDLVFEIYRSFGFDSVLIKLS
HCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
CCCCCCCCCCHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRMPVWLAPVQ
EEEEECCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEE
VAVMGITDRNAQTCQDVCKKLSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEECCCCC
QSGEVAVRTREGKDFGSMPLNSFISLLDEAIALKGRSGVS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA