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The map label for this gene is gadA [H]

Identifier: 124023504

GI number: 124023504

Start: 1570942

End: 1572381

Strand: Direct

Name: gadA [H]

Synonym: P9303_18041

Alternate gene names: 124023504

Gene position: 1570942-1572381 (Clockwise)

Preceding gene: 124023499

Following gene: 124023505

Centisome position: 58.56

GC content: 52.71

Gene sequence:

>1440_bases
ATGGCCCTGCATCGTTCTAATCCCCGCTCCAACGATCTGGAAGAACATGCCATTTGGGAAACCGTTGATGCATTGGTCTC
ACCATTACCACGCGCGACTTTTCCGCAAGAGTGCCGTCCTGCATTGACAACATTTAATTTGCTCAAGGAGGAGTTATTGC
TTGACGGCAACAGCAAACAAAATCTGGCCACCTTCTGTCAGACCTATGAAAGTCACCAGGTGGCTGATCTAATGGCCCTC
GCGGTTGACAAGAACCTGATCGACAAGGACGAATATCCCCAAACCGCAGAGATTGAACGCCGTTGTGTTTCGATGATGGC
TGATTTATGGCACGCCCCCGGTGAAGCTGTGGGCTGCTCCACCATCGGTAGCAGTGAGGCCGCCATGCTGGGTGGCTTGG
CTGCGAAATGGCGTTGGCGTGAACATCGCAAAGCGGCAGGTCAGTCGATCGATCGCCCCAACATGGTTTGCGGCAGTGTA
CAAATCTGCTGGACGAAATTTGCCCGCTACTGGGACGTTGAGTTGCGGGAGGTGGAGATGACCTCCGGCGAGCTCTGCAT
GTCTCCAGAACGAATGCTGGAGCAAGTGGATGAGAACACCATCGTTGTGGTGCCTACCCTGGGCGTCACTTATCACGGCC
TGTATGAAGACGTACAAGCCATCAGTCAGGCCCTCGATGAGCTGCAACAGCGAAAAGGACTCGACATTCCTATCCATGTG
GATGCAGCCAGCGGTGGCTTTCTTGCTCCTTTCTGTGCCCCTGATCTGGCTCCCTGGGATTTCCGTTTGCCGCGCGTGAA
GTCAATCAACTCCTCTGGCCATAAGTTCGGCTTAGCTCCTCTGGGTGTGGGTTGGGTTCTTTGGCGTGAGTCCAGTGATC
TTCCGGAAGGATTGGTTTTCCATGTGAGTTACCTAGGAGGCGATATGCCAACGTTTCAGATCAACTTCTCGCGGCCAGCG
GGGCAGGTGATCTCCCAGTACTACGACTTTGTCCGCCTAGGTCGCGATGGTTATCAAGCCATCCATGGAGCGAGCTATGC
CAATGCTCAATATGTTGCTCAAGAACTCAAAAAGCTAGGCCCATTTGAATTGATACACGATGGCAACCCAGCACGTGGCA
TCCCCACTGTGGTGTGGACATTGCGAGCCGGCCAAGAGCTTGGTTTCAACCTTTACGACCTCTCTGATCGACTGAGATTA
CGTGGTTGGCAAGTGCCAGCTTATCCATTCACAGGTGAGCTTGCTCATCAAGCCTTTCAGCGGATCTTGGTAAAACGCGA
TTTCTCACGTGAAATGGCTGATCTCCTACTTACCGACATCCGCAACGCCATAACTCATTTCGAGAGTCATCCTGTCAAGA
TCAGTCTTAACGCTACTGAAGCAGCGTCTACTAATCATCTCGGCCGTTCAATGGTTGAATCCCTTGATGCGCATGGATGA

Upstream 100 bases:

>100_bases
CATCAACCGACTCAGTTGTTTGTTATTGCACCTCCCATCTTGCAAGGCTTCCTTGCTCAAACAACGTCGTTAAGGTCAGA
CATAGACACAAGAAAAAAGC

Downstream 100 bases:

>100_bases
TGCCCCCCCATTGGGGGCTTTGAATTTAAAGCGGGAGCATCCTCCAAACCTTCAGTCGCATTATTCGAGTCCATGGGGCA
CGACAGCTTGGCGGCAAGAT

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MALHRSNPRSNDLEEHAIWETVDALVSPLPRATFPQECRPALTTFNLLKEELLLDGNSKQNLATFCQTYESHQVADLMAL
AVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCSTIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSV
QICWTKFARYWDVELREVEMTSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAISQALDELQQRKGLDIPIHV
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESSDLPEGLVFHVSYLGGDMPTFQINFSRPA
GQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLGPFELIHDGNPARGIPTVVWTLRAGQELGFNLYDLSDRLRL
RGWQVPAYPFTGELAHQAFQRILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNATEAASTNHLGRSMVESLDAHG

Sequences:

>Translated_479_residues
MALHRSNPRSNDLEEHAIWETVDALVSPLPRATFPQECRPALTTFNLLKEELLLDGNSKQNLATFCQTYESHQVADLMAL
AVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCSTIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSV
QICWTKFARYWDVELREVEMTSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAISQALDELQQRKGLDIPIHV
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESSDLPEGLVFHVSYLGGDMPTFQINFSRPA
GQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLGPFELIHDGNPARGIPTVVWTLRAGQELGFNLYDLSDRLRL
RGWQVPAYPFTGELAHQAFQRILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNATEAASTNHLGRSMVESLDAHG
>Mature_478_residues
ALHRSNPRSNDLEEHAIWETVDALVSPLPRATFPQECRPALTTFNLLKEELLLDGNSKQNLATFCQTYESHQVADLMALA
VDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCSTIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSVQ
ICWTKFARYWDVELREVEMTSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAISQALDELQQRKGLDIPIHVD
AASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESSDLPEGLVFHVSYLGGDMPTFQINFSRPAG
QVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLGPFELIHDGNPARGIPTVVWTLRAGQELGFNLYDLSDRLRLR
GWQVPAYPFTGELAHQAFQRILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNATEAASTNHLGRSMVESLDAHG

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=407, Percent_Identity=24.5700245700246, Blast_Score=100, Evalue=6e-21,
Organism=Escherichia coli, GI1787769, Length=413, Percent_Identity=59.8062953995157, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1789934, Length=413, Percent_Identity=59.8062953995157, Blast_Score=529, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17557272, Length=372, Percent_Identity=26.0752688172043, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25148342, Length=416, Percent_Identity=24.0384615384615, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543922, Length=329, Percent_Identity=26.1398176291793, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323905, Length=465, Percent_Identity=37.8494623655914, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6320500, Length=377, Percent_Identity=25.1989389920424, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355963, Length=384, Percent_Identity=23.4375, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24654344, Length=384, Percent_Identity=23.4375, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53519; Mature: 53388

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHRSNPRSNDLEEHAIWETVDALVSPLPRATFPQECRPALTTFNLLKEELLLDGNSKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
NLATFCQTYESHQVADLMALAVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCS
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
TIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSVQICWTKFARYWDVELREVEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHCCEEEEEEC
TSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAISQALDELQQRKGLDIPIHV
CCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESSDLPEGLVF
ECCCCCEECHHCCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCEEEEEECCCCCCCCCEE
HVSYLGGDMPTFQINFSRPAGQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLG
EEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCC
PFELIHDGNPARGIPTVVWTLRAGQELGFNLYDLSDRLRLRGWQVPAYPFTGELAHQAFQ
CEEEEECCCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
RILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNATEAASTNHLGRSMVESLDAHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALHRSNPRSNDLEEHAIWETVDALVSPLPRATFPQECRPALTTFNLLKEELLLDGNSKQ
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
NLATFCQTYESHQVADLMALAVDKNLIDKDEYPQTAEIERRCVSMMADLWHAPGEAVGCS
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
TIGSSEAAMLGGLAAKWRWREHRKAAGQSIDRPNMVCGSVQICWTKFARYWDVELREVEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHCCEEEEEEC
TSGELCMSPERMLEQVDENTIVVVPTLGVTYHGLYEDVQAISQALDELQQRKGLDIPIHV
CCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DAASGGFLAPFCAPDLAPWDFRLPRVKSINSSGHKFGLAPLGVGWVLWRESSDLPEGLVF
ECCCCCEECHHCCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCEEEEEECCCCCCCCCEE
HVSYLGGDMPTFQINFSRPAGQVISQYYDFVRLGRDGYQAIHGASYANAQYVAQELKKLG
EEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCC
PFELIHDGNPARGIPTVVWTLRAGQELGFNLYDLSDRLRLRGWQVPAYPFTGELAHQAFQ
CEEEEECCCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
RILVKRDFSREMADLLLTDIRNAITHFESHPVKISLNATEAASTNHLGRSMVESLDAHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]