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The map label for this gene is ksgA

Identifier: 124023319

GI number: 124023319

Start: 1417716

End: 1418558

Strand: Direct

Name: ksgA

Synonym: P9303_16171

Alternate gene names: 124023319

Gene position: 1417716-1418558 (Clockwise)

Preceding gene: 124023317

Following gene: 124023320

Centisome position: 52.85

GC content: 53.62

Gene sequence:

>843_bases
ATGGCCTTTTCCGGACATCACGCTCGCAAGCGCTTTGCTCAACACTGGCTGATTGATGCCGCTGTGTTGACCCAGATTCT
CGATGCTGCTGATGTTCAACCAGATGATCGTCTGCTGGAGGTAGGTCCTGGTCGTGGAGCACTCACTGAAAGGTTGTTGG
CCTCTTCTGCCTCTGCTGTTCATGCTGTTGAACTTGATCGCGATCTTGTCTCTGGGTTGAGGCAGCGATTTGCGGATCAA
GCCCGCTTCAGCTTGCAAGAAGGTGATGTGTTGTCTGTTCCATTGACTCTTGCTGATGGCCGTGCTGCGAACAAAGTGGT
GGCCAATATTCCCTATAACATCACCGGGCCTCTGCTTGAGCGTCTTCTCGGTCGCTTGGATCGACCTGTTGATCATCCCT
ATCAACGCCTTGTGCTTCTTTTGCAAAAGGAAGTCGCTCAACGGATTCGTGCCTTGCCCGGGCAGAGCTGTTTTAGTGCT
CTAAGCGTGCGCCTGCAGTTGCTCGCTCATTGCACAACTGTTTGCCCAGTGCCGCCGCGCTCTTTTAAACCGCCGCCGAA
AGTTCATTCTGAGGTCATCTTGATTGAACCTCTTGCACCTGAACAGCGCCTTGAACCACTGCTCGCCAAACGTGTTGAGT
CTTTGCTGCGTCAGGCTTTTTTGGCCAGGCGCAAGATGCTGCGCAACACTCTTGCCAAGGTTCTTCCTGCTGCTGAACTG
AATGCTCTTGCTGATGATCTTGGCATCAGCCTTCAGCAACGCCCCCAAGAACTGTCCCCTGCCACTTGGGTGGAATTGGC
CAGGGGTTTGAATCGGGCGGATCTAGTAGACCCTGAGCCATGA

Upstream 100 bases:

>100_bases
TGACTGGAACAGCGAGTTTGACTCCGAGTTCTGTCTGCAGCTTTTTTTGATCTTCAATCAATTGCGCCGCCCTCTATACC
GATTGGTGCAAAGTTGAGTG

Downstream 100 bases:

>100_bases
GCATATCTAGCCCTTCTGCTGCTTCAGGGCATCTGGTTAGCGTCTCGGCGCCAGCCAAGATCAATCTCCATCTTGAGGTG
CTTGGCCTCAGGTCTGATGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAVHAVELDRDLVSGLRQRFADQ
ARFSLQEGDVLSVPLTLADGRAANKVVANIPYNITGPLLERLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSA
LSVRLQLLAHCTTVCPVPPRSFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP

Sequences:

>Translated_280_residues
MAFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAVHAVELDRDLVSGLRQRFADQ
ARFSLQEGDVLSVPLTLADGRAANKVVANIPYNITGPLLERLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSA
LSVRLQLLAHCTTVCPVPPRSFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP
>Mature_279_residues
AFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAVHAVELDRDLVSGLRQRFADQA
RFSLQEGDVLSVPLTLADGRAANKVVANIPYNITGPLLERLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSAL
SVRLQLLAHCTTVCPVPPRSFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAELN
ALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=218, Percent_Identity=36.2385321100917, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=27.4576271186441, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1786236, Length=273, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=35.3211009174312, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=27.6094276094276, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=279, Percent_Identity=29.0322580645161, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358017, Length=256, Percent_Identity=35.15625, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357273, Length=292, Percent_Identity=27.7397260273973, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PROM3 (A2CA51)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017626.1
- ProteinModelPortal:   A2CA51
- SMR:   A2CA51
- STRING:   A2CA51
- GeneID:   4778136
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_16171
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30905; Mature: 30774

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 15-15 BINDING 17-17 BINDING 42-42 BINDING 64-64 BINDING 89-89 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHH
HAVELDRDLVSGLRQRFADQARFSLQEGDVLSVPLTLADGRAANKVVANIPYNITGPLLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCCCCCCCCHHHHH
RLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSALSVRLQLLAHCTTVCPVPPR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP
HHHHHHHCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AFSGHHARKRFAQHWLIDAAVLTQILDAADVQPDDRLLEVGPGRGALTERLLASSASAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHH
HAVELDRDLVSGLRQRFADQARFSLQEGDVLSVPLTLADGRAANKVVANIPYNITGPLLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCCCCCCCCHHHHH
RLLGRLDRPVDHPYQRLVLLLQKEVAQRIRALPGQSCFSALSVRLQLLAHCTTVCPVPPR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SFKPPPKVHSEVILIEPLAPEQRLEPLLAKRVESLLRQAFLARRKMLRNTLAKVLPAAEL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
NALADDLGISLQQRPQELSPATWVELARGLNRADLVDPEP
HHHHHHHCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA