Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 124023317

GI number: 124023317

Start: 1415651

End: 1417006

Strand: Direct

Name: gdhA [H]

Synonym: P9303_16151

Alternate gene names: 124023317

Gene position: 1415651-1417006 (Clockwise)

Preceding gene: 124023316

Following gene: 124023319

Centisome position: 52.77

GC content: 43.66

Gene sequence:

>1356_bases
ATGTCTGATGCTATTAGCCATGAGATAGATCATTTCATGAAAGGTCTGATGAAGAGAAATCCTGGAGAGCCTGAGTTTCA
TCAAGCCGTCCATGAAGTTGTTGAAACTCTAATTCCATTCATTCTTTCCAACCGTGTTTACAAAGATGCACAGATTCTGG
AGCGGATGACGGAACCTGACCGCGTCGTCATCTTCCGAGTCTGCTGGGAAGATGACGCAGGCAATATCCGCACAAATCGA
GCGTGGCGTGTACAGTTTAATAATTCCATTGGACCTTATAAAGGTGGAATACGTTTCCATCCCAATGTCACCTTGAGTGT
CCTGAAATTTTTAGGTTTTGAACAGACCTTTAAAAACAGTCTTACTGGTCTGCCGATGGGTGGAGGGAAGGGTGGATCTA
ACTTTAATCCTAAAGGTAAAAGTGACCGGGAGATAATGCGATTTTGTCAATCTCTGATGATTGAACTTCATAGACATATT
GGTGAAGACACAGATGTACCTGCTGGTGATATCGGAGTCGGTTCTCGTGAAATTAGTTATATGTTCGGTCAGTACAAGCG
CCTTGAAAATCGGTTTAGTGGAATCTTGACTGGCAAGGGGCTGTCATTCGGTGGAAGTCTTGTTCGTACTGAAGCGACGG
GTTACGGATGTGCTTATTTCTGTGAAAATATGCTCAATCATATAGAAGACTCTCTCGTTGACAAAATATGTTCCATCTCC
GGTTCTGGGAACGTAGCCCTCTACGCAGTTGAAAAGGCCATTGAGCTAGGAGCGAAAGTTGTTACTCTTTCTGACTCAGA
TGGTTTTGTACACGTCCCAAACGGCATTAATCTCGAGATGCTTGCGTTTGTCAAAGAGCTAAAGACTGTTCGCCGTGGGC
GAATCCATGAATTTGCTAACCACTACAAAGGCGTTGATTTTTACGCAGGAAAGCGTCCATGGAATGTTCCCTGTGATATT
GCCTTCCCTTGTGCTACTCAAAATGAGATTAACCGAGCAGATTCACAGGAACTGATCAACAATGGTGTTAAAGCAGTTGT
TGAAGGTGCGAACATGCCAACCAATCTTGACGGCATTCATGAATTTCGTCATGCAAAAGTAATACTTGCTCCTGCCAAGG
CTGCAAATGCTGGGGGTGTCGCAGTCTCTGGCCTTGAGCAGAGTCAGAATGCATTGCGTCTATCTTGGAGTCGTGAAGAG
GTCGATCAACGCTTAAAGTCAATTATGTTGGAAATTCACTCCAAATGTGTTCAGCATAGTCATAAATACGAAGACTACAC
TGATTACATTACAGGAGCCAATATTGCAGGATTTAAGAAAGTTGCTGATGCAATGCTGGCTTATGGAATTGTTTGA

Upstream 100 bases:

>100_bases
GTCATACTTTTAAGCTGATCTGGATAACATTCTGTATTAAAGTAACTTTAGGCGCTTGCCATGACATCATACTAAGTAAC
TTCTTGAGAAAGGACAGCTG

Downstream 100 bases:

>100_bases
TTTTATATCAGTGGATACATATGATTATTTTCGGCTGAAGTCTAATCAAGAGGGACAAGCCCGGTTTCATGATTTTGGCA
CTCTGTGTATGGGGTTGATG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSDAISHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPDRVVIFRVCWEDDAGNIRTNR
AWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNSLTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHI
GEDTDVPAGDIGVGSREISYMFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFVKELKTVRRGRIHEFANHYKGVDFYAGKRPWNVPCDI
AFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIHEFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREE
VDQRLKSIMLEIHSKCVQHSHKYEDYTDYITGANIAGFKKVADAMLAYGIV

Sequences:

>Translated_451_residues
MSDAISHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPDRVVIFRVCWEDDAGNIRTNR
AWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNSLTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHI
GEDTDVPAGDIGVGSREISYMFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFVKELKTVRRGRIHEFANHYKGVDFYAGKRPWNVPCDI
AFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIHEFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREE
VDQRLKSIMLEIHSKCVQHSHKYEDYTDYITGANIAGFKKVADAMLAYGIV
>Mature_450_residues
SDAISHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPDRVVIFRVCWEDDAGNIRTNRA
WRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNSLTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHIG
EDTDVPAGDIGVGSREISYMFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSISG
SGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFVKELKTVRRGRIHEFANHYKGVDFYAGKRPWNVPCDIA
FPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIHEFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREEV
DQRLKSIMLEIHSKCVQHSHKYEDYTDYITGANIAGFKKVADAMLAYGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=30.0291545189504, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI4885281, Length=343, Percent_Identity=30.0291545189504, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1788059, Length=446, Percent_Identity=57.6233183856502, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17544676, Length=388, Percent_Identity=29.1237113402062, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=48.0088495575221, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=47.7973568281938, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45549226, Length=395, Percent_Identity=28.1012658227848, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI45553475, Length=395, Percent_Identity=28.1012658227848, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24649283, Length=322, Percent_Identity=31.9875776397516, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649460, Length=299, Percent_Identity=28.7625418060201, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 50049; Mature: 49917

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAISHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPD
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RVVIFRVCWEDDAGNIRTNRAWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNS
EEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHC
LTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHIGEDTDVPAGDIGVGSREISY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
MFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
HHHHHHHHHHHHHCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFVKELKTVRRGRIHEFAN
CCCCEEEEEHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HYKGVDFYAGKRPWNVPCDIAFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIH
HCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
EFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREEVDQRLKSIMLEIHSKCVQHS
HHHHCEEEEECCCCCCCCCEEEECHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
HKYEDYTDYITGANIAGFKKVADAMLAYGIV
CCCCHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDAISHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPD
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RVVIFRVCWEDDAGNIRTNRAWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNS
EEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHC
LTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHIGEDTDVPAGDIGVGSREISY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
MFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
HHHHHHHHHHHHHCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFVKELKTVRRGRIHEFAN
CCCCEEEEEHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HYKGVDFYAGKRPWNVPCDIAFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIH
HCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
EFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREEVDQRLKSIMLEIHSKCVQHS
HHHHCEEEEECCCCCCCCCEEEECHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
HKYEDYTDYITGANIAGFKKVADAMLAYGIV
CCCCHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]