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The map label for this gene is dnaG [H]

Identifier: 124023293

GI number: 124023293

Start: 1395411

End: 1397465

Strand: Direct

Name: dnaG [H]

Synonym: P9303_15911

Alternate gene names: 124023293

Gene position: 1395411-1397465 (Clockwise)

Preceding gene: 124023292

Following gene: 124023296

Centisome position: 52.02

GC content: 53.67

Gene sequence:

>2055_bases
ATGCCCGTCCCCCGTCTTCACCCGCGCACGATCGAGGCCGTCAAGGAGCGTGCCGACATTGTTGATGTCGTTGGCGAACA
TGTGGTTCTAAAGAAAAAGGGCCGTGAATTCGTAGGGATCTGTCCGTTCCATGACGACAGCAAACCATCGATGACGGTCT
CGCCAGCGAAGCAGTTTTATTACTGCTTCTCTTGTGGAGCTGGTGGAAACTCGATCAAGTTCCTGATGGAGTTTCAGCGC
CAGAGTTTTGGTGATGTGGTGCTGGAGCTTGCTCGCAAGTATCAACTTCCTGTTGAGACAGTCGAGGGGCCCCAGCAGGA
ACGACTACGTCAGCAGCTCTCTCGTCGAGACAAGCTTCATCGTGCGCTTGCATTGGCGGCCGGTTGGTTTCGTGGCCAGC
TGCGTACGCCAGTTGGCGCAAATGCGCTGAATTACCTCACTGAGAAGCGGGGCCTTAGTGAAGTCACCCTGGAATCCTTC
GAGCTTGGCTATGCCCCTGAGCAATGGGATGGTTTACTCAAGCACCTCCAGCAGGTGGAAGGTTTGTCTGCTGAATTACT
GGAAGCCGCAGGGCTTGTTGTGGCGCGCAAGGGTGGAGGAGGTTTTTACGATCGCTTTCGTCATCGTGTGATGGTGCCGA
TCCACGATCGCCAGGGACGGGTGATTGGTTTCGGCGGACGCAGTCTTGATGGCTCAGAGCCGAAGTACCTCAACTCTCCT
GAGACAGACGTGTTCGAGAAAGGCAAGCATTTGTTTGGACTGGATCGGGCGGCAAATGCCATCCGTAAGGATGACCGGGC
TGTGGTAGTCGAAGGATATTTCGATGTCATTGCATTGCATGCTGCAGGTGTCACCAATTCAGTGGCCTCCTTAGGCACTG
CTTTGAGTAGTCAGCAGATCACCCAGATCTGCCGTTGCAGCGAAGGGAAGCGGATCGTGCTTAATTTTGATGCTGATGGA
GCCGGTGTTCGTGCTGCGAACCGGGCCATTGGCGAAGTTGAGCAACTGGCTTTGCAAGGCCAGTTGGAATTACGAGTGCT
TCATTTGCCTTCTGGCAAAGACCCCGATGAATTTCTCAAAGATCATGGTGCTGGCGATTACAGGGCTCTATTAGATCAGT
CACCTCTTTGGCTTGATTGGCAGATTCAACAGGTTTTGGAGGGCCGTGATTTAAGTCAAGCGGATCAGTTTCAGCAGGCT
GTGAGTGGCCTGGTTGCATTGCTCGGCAAGTTGCCCCAGTCAGCCGTACGGACCCACTACCTGCAACAGGTTGCCGAACG
CTTAAGCGGTGGACAGGGCCGTTTGGCCTTGCAGTTGGAGGAAGATCTGCGCCAGCAAGTGAAAGGGCAGCGCTGGCATG
GCCGCTCAAGTCGCCATGAGCAACCTGGCGAGGCCAGTCAACGGGAGCGCAGCGAGGCGGAAATCCTGAGGTTATATCTC
CATTGCTCAAATCATCGGGCTGCCATTCGCAGGGAGTTACGACAGCGAGAGCTGGAGGATTTTGCCTTACAACATCACCG
TTTGCTTTGGGCGGCGATCACTGATTTGGAGGAAACCAATCTTGGTGCAGGACGTTTAGAGGCGATCAGTCGAGGAACTG
ACTCTGGTCATGATTTGGAGGATATTGAGTTACCACGGCTGCTTACCGATCAGTTGCTCCTGGAGAACAGTGCTCTAGTC
GCTCGTCTTACCCCTTTATTAGAGCCTGGCGAGGTTCAGTTGGCTGCTATGGCTAAGCCACTGCTTCAGCTCCGGGGCAC
AACAGCGGCTCTAGAACGCCAGAAATCCCTAAAGCGTTGTCGTCATCTTCTTGAGGCCTGGGGTAGTCAGCGTCTTGAAA
CTCTTGAGCGTTGTATTGCTGCTTTGATTGAAGAAGAGCGACAGCAACCTACTGAATCGGTCGATATGGAAATGCGTATC
GAAGCAATGTTTGAGGAGCTCAACAGCGATGCATTGCGTTTCCAGGAGCTTTATTACAGCGAACGCAAGCACATTGGTCA
CTTAGATCAGCAGCGTTGTGGTGGCTTCTCAGGCCAGGATGTCATTCCGGCTTAG

Upstream 100 bases:

>100_bases
TTGAGTTCTTATTGATCTCGGCCTTGCTTGTCGTATGGATTGATTTTGCTGATCATGTTCTCTTCTCGACCTCTCGCGGC
GCAACCAGCAAAATAAGGTC

Downstream 100 bases:

>100_bases
GTCATCAGTCTGTTGATGCGCTTACAAAGCTATAAAACTGACGATCAGCATGGAGAAGTCCTCATGAAAACAATTTAGAG
GAGATAAATATCTCTTAAGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MPVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFQR
QSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLHRALALAAGWFRGQLRTPVGANALNYLTEKRGLSEVTLESF
ELGYAPEQWDGLLKHLQQVEGLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQITQICRCSEGKRIVLNFDADG
AGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLKDHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQA
VSGLVALLGKLPQSAVRTHYLQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLEDIELPRLLTDQLLLENSALV
ARLTPLLEPGEVQLAAMAKPLLQLRGTTAALERQKSLKRCRHLLEAWGSQRLETLERCIAALIEEERQQPTESVDMEMRI
EAMFEELNSDALRFQELYYSERKHIGHLDQQRCGGFSGQDVIPA

Sequences:

>Translated_684_residues
MPVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFQR
QSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLHRALALAAGWFRGQLRTPVGANALNYLTEKRGLSEVTLESF
ELGYAPEQWDGLLKHLQQVEGLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQITQICRCSEGKRIVLNFDADG
AGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLKDHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQA
VSGLVALLGKLPQSAVRTHYLQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLEDIELPRLLTDQLLLENSALV
ARLTPLLEPGEVQLAAMAKPLLQLRGTTAALERQKSLKRCRHLLEAWGSQRLETLERCIAALIEEERQQPTESVDMEMRI
EAMFEELNSDALRFQELYYSERKHIGHLDQQRCGGFSGQDVIPA
>Mature_683_residues
PVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFQRQ
SFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLHRALALAAGWFRGQLRTPVGANALNYLTEKRGLSEVTLESFE
LGYAPEQWDGLLKHLQQVEGLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSPE
TDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQITQICRCSEGKRIVLNFDADGA
GVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLKDHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQAV
SGLVALLGKLPQSAVRTHYLQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYLH
CSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLEDIELPRLLTDQLLLENSALVA
RLTPLLEPGEVQLAAMAKPLLQLRGTTAALERQKSLKRCRHLLEAWGSQRLETLERCIAALIEEERQQPTESVDMEMRIE
AMFEELNSDALRFQELYYSERKHIGHLDQQRCGGFSGQDVIPA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=428, Percent_Identity=37.8504672897196, Blast_Score=281, Evalue=7e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 76714; Mature: 76583

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFY
CCCCCCCHHHHHHHHHHHHHHHHHHCHHEEECCCCCEEEEECCCCCCCCCEEECCHHHEE
YCFSCGAGGNSIKFLMEFQRQSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLH
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHH
RALALAAGWFRGQLRTPVGANALNYLTEKRGLSEVTLESFELGYAPEQWDGLLKHLQQVE
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
CCHHHHHHHCCEEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQI
CHHHHHCCCHHCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TQICRCSEGKRIVLNFDADGAGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLK
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
DHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQAVSGLVALLGKLPQSAVRTHY
HCCCCHHHHHHCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
DIELPRLLTDQLLLENSALVARLTPLLEPGEVQLAAMAKPLLQLRGTTAALERQKSLKRC
CCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RHLLEAWGSQRLETLERCIAALIEEERQQPTESVDMEMRIEAMFEELNSDALRFQELYYS
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
ERKHIGHLDQQRCGGFSGQDVIPA
HHHHCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFY
CCCCCCHHHHHHHHHHHHHHHHHHCHHEEECCCCCEEEEECCCCCCCCCEEECCHHHEE
YCFSCGAGGNSIKFLMEFQRQSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLH
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHH
RALALAAGWFRGQLRTPVGANALNYLTEKRGLSEVTLESFELGYAPEQWDGLLKHLQQVE
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
CCHHHHHHHCCEEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQI
CHHHHHCCCHHCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TQICRCSEGKRIVLNFDADGAGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLK
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
DHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQAVSGLVALLGKLPQSAVRTHY
HCCCCHHHHHHCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
DIELPRLLTDQLLLENSALVARLTPLLEPGEVQLAAMAKPLLQLRGTTAALERQKSLKRC
CCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RHLLEAWGSQRLETLERCIAALIEEERQQPTESVDMEMRIEAMFEELNSDALRFQELYYS
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
ERKHIGHLDQQRCGGFSGQDVIPA
HHHHCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA