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The map label for this gene is met17 [H]

Identifier: 124023024

GI number: 124023024

Start: 1126436

End: 1127764

Strand: Reverse

Name: met17 [H]

Synonym: P9303_13191

Alternate gene names: 124023024

Gene position: 1127764-1126436 (Counterclockwise)

Preceding gene: 124023025

Following gene: 124023023

Centisome position: 42.04

GC content: 50.49

Gene sequence:

>1329_bases
TTGACTTCACATCGCTTCGAAACCCTCCAGCTTCATGCCGGCCAGGTAGCTGATCCTGTCACGAACTCACGAGCTGTACC
CATTTATCAAACCAGTTCGTATGTCTTTAATGACGCTGAACATGGTGCCAACCTGTTTGGTCTTAAAGAATTCGGCAACA
TTTACACCCGTCTGATGAACCCCACGACGGATGTGTTTGAAAAGCGTGTTGCGGCTCTTGAGGGAGGGATTGCTGCTGTA
GCGACAGCTTCCGGACAATCCGCCCAGTTCCTGGCAATTACAAACTGCATGCAGGCTGGTGACAATCTTGTCTCAACATC
CTTCTTGTATGGAGGAACCTACAATCAATTCAAGGTGCAGTTTCCCCGCCTTGGCATTGATGTGAAGTTTGCGGATGGTG
ATGATGTTGATAGCTTTGCCACACAAATCGATGCCAACACAAAAGCAATTTATGTAGAGTCGATGGGGAACCCTCGCTTC
AATATCCCTGACTTTAAAGGCCTTTCAGGCTTAGCAAAAGACATGGGAATCCCCTTAATTGTTGACAACACCCTTGGAGC
AGCTGGTGCTTTGATTCGGCCCATAGAGCATGGCGCTGATGTGGTGGTGGAAAGTGCGACCAAATGGATTGGAGGCCATG
GCACCAGCCTTGGTGGAGTGCTTGTGGATGCTGGCACCTTTAACTGGGGCAATGGCAAGTTCCCACTAATGAGTGAGCCA
AGCGCGGCATATCACGGTCTGGTGCACTGGGATGCTTTCGGGTTCGGCAGCGACATCTGCTCCATGCTCGGCGTTCCAAG
CAATCGCAATGTGGCCTTTGCACTGCGAGCTCGAGTTGAGGGCCTGCGCGACTGGGGAGCAGCTCTTAGTCCATTCAATT
CATTCCTACTTTTGCAAGGGCTCGAAACACTGAGTTTGCGAGTGGAGCGCCATGCATCCAATGCCATGGCTCTTGCTACA
TGGCTTCAGGATCATCCCAAGGTTGCCAGTGTCAACTATCCCGGCCTGAAAAATGACCCATACCACGCACAAGCCAAAAC
ATACCTAACGAATCGAGGCATGGGATGCATGCTGATGTTCTCCCTTAAAGGAGGCTTTGATGATGCCGTGAGCTTCATCA
ATGGTCTTGAATTAGCTAGCCATCTTGCCAATGTTGGTGATGCCAAGACCTTGGTGATTCATCCTGCATCCACCACGCAT
CAACAACTTTCTGCTCAAGAGCAGGAATCTGCAGGAGTCACTCCCACAATGGTTAGGGTATCTGTTGGCCTTGAACACAT
CGAAGACATCAAGGCTGATTTTGAACAGGCCCTAGCGGTCATCAGCTGA

Upstream 100 bases:

>100_bases
CCATCCTTAGCAAAGACAGATAAAGCTGATCTACCTGACGACTGGAACCGCGCCTGAGACTAAGTTGCTAGAAAGAAGAG
ACCTTCCTGTTTACGTGTAG

Downstream 100 bases:

>100_bases
GACTGGAGGCATAGATGGCGCTGATCCTGCCACGCAGTTATCACAAGATCGCCGCGGTGGAGAAGAACCGGATCTCATGG
ATAGAGCCGGAACTTGCAGA

Product: O-acetyl homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGGIAAV
ATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRF
NIPDFKGLSGLAKDMGIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQGLETLSLRVERHASNAMALAT
WLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMFSLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTH
QQLSAQEQESAGVTPTMVRVSVGLEHIEDIKADFEQALAVIS

Sequences:

>Translated_442_residues
MTSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGGIAAV
ATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRF
NIPDFKGLSGLAKDMGIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQGLETLSLRVERHASNAMALAT
WLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMFSLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTH
QQLSAQEQESAGVTPTMVRVSVGLEHIEDIKADFEQALAVIS
>Mature_441_residues
TSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGGIAAVA
TASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRFN
IPDFKGLSGLAKDMGIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEPS
AAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQGLETLSLRVERHASNAMALATW
LQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMFSLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQ
QLSAQEQESAGVTPTMVRVSVGLEHIEDIKADFEQALAVIS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=437, Percent_Identity=30.2059496567506, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI25453487, Length=436, Percent_Identity=27.5229357798165, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI299473759, Length=437, Percent_Identity=27.2311212814645, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1790375, Length=410, Percent_Identity=30.7317073170732, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1789383, Length=450, Percent_Identity=22, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=453, Percent_Identity=29.8013245033113, Blast_Score=191, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=439, Percent_Identity=30.2961275626424, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=30.2564102564103, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=455, Percent_Identity=50.1098901098901, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319307, Length=443, Percent_Identity=31.1512415349887, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321254, Length=466, Percent_Identity=24.6781115879828, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321170, Length=350, Percent_Identity=26.5714285714286, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922554, Length=443, Percent_Identity=28.8939051918736, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47347; Mature: 47216

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMN
CCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCEECCHHCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRVAALEGGIAAVATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRFNIPDFKGLSGLAKDMGIPLI
ECEECEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCEE
VDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
EECCCCCCHHHHHHHHCCCHHEEHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
LETLSLRVERHASNAMALATWLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMF
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEE
SLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQQLSAQEQESAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
SVGLEHIEDIKADFEQALAVIS
HHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSHRFETLQLHAGQVADPVTNSRAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMN
CCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCEECCHHCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRVAALEGGIAAVATASGQSAQFLAITNCMQAGDNLVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIDVKFADGDDVDSFATQIDANTKAIYVESMGNPRFNIPDFKGLSGLAKDMGIPLI
ECEECEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCEE
VDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVLVDAGTFNWGNGKFPLMSEP
EECCCCCCHHHHHHHHCCCHHEEHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICSMLGVPSNRNVAFALRARVEGLRDWGAALSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
LETLSLRVERHASNAMALATWLQDHPKVASVNYPGLKNDPYHAQAKTYLTNRGMGCMLMF
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEE
SLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQQLSAQEQESAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
SVGLEHIEDIKADFEQALAVIS
HHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]