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The map label for this gene is pdxJ

Identifier: 124022665

GI number: 124022665

Start: 863107

End: 863844

Strand: Direct

Name: pdxJ

Synonym: P9303_09561

Alternate gene names: 124022665

Gene position: 863107-863844 (Clockwise)

Preceding gene: 124022659

Following gene: 124022667

Centisome position: 32.17

GC content: 53.12

Gene sequence:

>738_bases
ATGGCCAGCCTCGGGGTCAACATCGACCACATTGCCAATGTGCGGCAGGCTCGCCAAACCGTGGAACCCGATCCAGTGCC
GATGGCTCTATTGGCGGAACTTGGTGGAGCCGATGGCATCACAGTTCACTTAAGAGAAGATCGCCGTCATATTCAGGATC
GAGACCTGGAACTGCTTCGGGCAACAGTGCGCAGTCGTCTCAATTTGGAGATGGCAGCCACTCCTGAAATGGTGGGAATT
GCTTTAAAAATTCAACCTGACATGGTGACACTCGTACCAGAACGACGGCAGGAGGTGACCACAGAAGGAGGTTTGGATGT
TGCTGCCCAGCAGGGATCCTTGAAAGGCATGGTTGATCAGCTGCAGGTTGCAGGGATTCCCGTCAGCTTGTTTGTAGATC
CTGTTTCTCAACAACTGGAGGCAGCTTGTAAGAGTGGTGCTCGTTGGGTGGAGCTGCACACTGGCGCTTACGCCGAAGCC
TGTTGGGCTAATCAATCCTTTGAACTTGCACGCTTGAATGAAGCGACAGCAAGAGCCCGCAGCCTTGGCTTACGGGTGAA
TGCTGGGCATGGCCTCACTTATCAGAATGTAGAAGCCGTGGCTGCGATTGAAGGGATTGAAGAATTGAATATTGGCCATA
CGATTGTGGCTCGCTCGATCGCTGTAGGCCTTCAAGAAGCTGTAAGAGAGATGAAGCGTCTTGTCCAGAATCCCCGACGA
GAGCCTCTATTCGGGTAG

Upstream 100 bases:

>100_bases
ACCCAATGTTGATTGGGCAGTTGAACCGGCGGTCAAATCCCTTAGAGAATAGAAGGCAGTCAATTATCCCCAATAGGATC
AGGCCTGTTTCCCTTTCTTC

Downstream 100 bases:

>100_bases
TCAGCAAGGATGAGTAGGCATTATTTTGTCTCTAAAATTAATGCTTACTCAGTAAATATTTGATTCAGAACTTGTTGACT
GAGTAAAAAAGATGAATCTG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MASLGVNIDHIANVRQARQTVEPDPVPMALLAELGGADGITVHLREDRRHIQDRDLELLRATVRSRLNLEMAATPEMVGI
ALKIQPDMVTLVPERRQEVTTEGGLDVAAQQGSLKGMVDQLQVAGIPVSLFVDPVSQQLEAACKSGARWVELHTGAYAEA
CWANQSFELARLNEATARARSLGLRVNAGHGLTYQNVEAVAAIEGIEELNIGHTIVARSIAVGLQEAVREMKRLVQNPRR
EPLFG

Sequences:

>Translated_245_residues
MASLGVNIDHIANVRQARQTVEPDPVPMALLAELGGADGITVHLREDRRHIQDRDLELLRATVRSRLNLEMAATPEMVGI
ALKIQPDMVTLVPERRQEVTTEGGLDVAAQQGSLKGMVDQLQVAGIPVSLFVDPVSQQLEAACKSGARWVELHTGAYAEA
CWANQSFELARLNEATARARSLGLRVNAGHGLTYQNVEAVAAIEGIEELNIGHTIVARSIAVGLQEAVREMKRLVQNPRR
EPLFG
>Mature_244_residues
ASLGVNIDHIANVRQARQTVEPDPVPMALLAELGGADGITVHLREDRRHIQDRDLELLRATVRSRLNLEMAATPEMVGIA
LKIQPDMVTLVPERRQEVTTEGGLDVAAQQGSLKGMVDQLQVAGIPVSLFVDPVSQQLEAACKSGARWVELHTGAYAEAC
WANQSFELARLNEATARARSLGLRVNAGHGLTYQNVEAVAAIEGIEELNIGHTIVARSIAVGLQEAVREMKRLVQNPRRE
PLFG

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=237, Percent_Identity=58.2278481012658, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_PROM3 (A2C897)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016972.1
- ProteinModelPortal:   A2C897
- SMR:   A2C897
- STRING:   A2C897
- GeneID:   4778633
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_09561
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26657; Mature: 26526

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 190-190 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLGVNIDHIANVRQARQTVEPDPVPMALLAELGGADGITVHLREDRRHIQDRDLELLR
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHH
ATVRSRLNLEMAATPEMVGIALKIQPDMVTLVPERRQEVTTEGGLDVAAQQGSLKGMVDQ
HHHHHHCCEEEECCCCCEEEEEEECCCEEEECCHHHHHHCCCCCCEEECCCCCHHHHHHH
LQVAGIPVSLFVDPVSQQLEAACKSGARWVELHTGAYAEACWANQSFELARLNEATARAR
HHHCCCCCEEEECHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHH
SLGLRVNAGHGLTYQNVEAVAAIEGIEELNIGHTIVARSIAVGLQEAVREMKRLVQNPRR
HHCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EPLFG
CCCCC
>Mature Secondary Structure 
ASLGVNIDHIANVRQARQTVEPDPVPMALLAELGGADGITVHLREDRRHIQDRDLELLR
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHH
ATVRSRLNLEMAATPEMVGIALKIQPDMVTLVPERRQEVTTEGGLDVAAQQGSLKGMVDQ
HHHHHHCCEEEECCCCCEEEEEEECCCEEEECCHHHHHHCCCCCCEEECCCCCHHHHHHH
LQVAGIPVSLFVDPVSQQLEAACKSGARWVELHTGAYAEACWANQSFELARLNEATARAR
HHHCCCCCEEEECHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHH
SLGLRVNAGHGLTYQNVEAVAAIEGIEELNIGHTIVARSIAVGLQEAVREMKRLVQNPRR
HHCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EPLFG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA