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The map label for this gene is trm1 [H]

Identifier: 124022558

GI number: 124022558

Start: 769809

End: 771083

Strand: Direct

Name: trm1 [H]

Synonym: P9303_08491

Alternate gene names: 124022558

Gene position: 769809-771083 (Clockwise)

Preceding gene: 124022555

Following gene: 124022559

Centisome position: 28.7

GC content: 56.39

Gene sequence:

>1275_bases
ATGGGATTCGCCCCAGTCAGCACCTGTCTCTTGAATGGCCCGCTCGGCAGTCTGGAAGTCAGTTGCAAACAAAGCGGCTT
GAACCAACCCACTACCCACTATCGCGAAGGCTCAGTCGTTTTGGAGCCAGGAGGTGGTTTCTTCCGCCCAGAGTCTCGTC
CTTCCCGTGATCTTTCGGTTTTATTGGCTGCTTTTCAGCAGCAGAGGAGAGAGAAGTTTTTGCCTGCTTTGCACTGGTTG
GACCTTATGGCTGGTTGCGGTATTCGTGCTTTGCGCTGGGGGTTGGAGGCTGTTAAACCCTCATTGTTGGCGTCAGGAGT
AGAGCAGCTGCCAGAGCTGTGGGTGAATGATGCCGATCTTGAGCGCGCTTCTCTTTTGGAGAGGAATCTGGCCCCTCTGC
GCCAAGAGCAAATCTCGCTAAAGCTTTTTCATCAGCCCGCTGAGGTCCTCCTCAGCCGTGCCTACCTCGATCAGTGTCGT
TTTGATCTGATTGATCTCGATTGTTTTGGTTGTCCGAATGCGTTTCTGCAACCTGTGTTGCAGGTTCTCGCTTCTGAGGG
GCTGTTGCTGCTTGCAAGCAGTGATGGTCGCTCTCCCACAGGCCATGACCGGCCAGGGGCAATTCGCAGTTTGGCTGCTG
CGGCTCGGGTGCATCCAGCCAGCTGGGAGATCGCTTTGAGGCTGCAGCTTGGGGTTCTTGCACGACATGCCTGGCTGCTA
GGGCGTGGTTTGGAGCCCCTGGTTAGCTTTAGTGAAGGTCGTACTTTTCGTGTTGCGGTAAGGATCAAGCGTCGACTTGC
TGCCGGTGAGGAGAGCCAGCTTGGGCTTTTAGCTCGCTGTGAGGCTTGTGGTGCTCAGCTTGCTCAACCACTGCTCTCCC
TGCAGGGATGGTCAGGGTGTATGTGTTCTCAAGGAGGTGGCCGCTGGGCGGTGAGCGGTCCCCTTTGGCTGGGCCCCCTT
CAGTCGCCAGATGTGTTGGCCGATTTGTTGGTGTTAGCAGAACAGTCGATCGTTCCGATCGCATCCGCCACACTTCGGCT
GTTGCATCGCTTGCAGTCTGACATCGGCTTGCCGGTCTATTGCTGGTCCACAGCTGAACTGGCCAAGCGTCTGGCTTTGT
CTGGCCCGCCGTCTGTTTTGGCATTGGTCAAGGCTCTGCGTTCTGCTGGCTATGAGGCTTGCTGCAGTGCCGTTATGGCA
GGACAACTACGCACCGATGCGACAGTGACCGAGCTTTTGCGGATTTGCCACGAGATGTGTCAGGAAGGGCGTTAA

Upstream 100 bases:

>100_bases
GAGCGCTGCTGGCACCGAATCCGTGGATGAGCACGAGTGGACGTGCGTCCTCTGCTCCCAGTACACGCCAGTGACAACGT
AGGCCTTGCCAATACCAATC

Downstream 100 bases:

>100_bases
ATGGTGTGCGTGATTCACATGCCCATTCATGGCTTCGGAGATTTTCGGGATTGCTGCCGTCTTCTGGGTGTTGATCCCAG
TTGGTCTTGCAGGTGGTGCT

Product: N2,N2-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSVLLAAFQQQRREKFLPALHWL
DLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADLERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCR
FDLIDLDCFGCPNAFLQPVLQVLASEGLLLLASSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
GRGLEPLVSFSEGRTFRVAVRIKRRLAAGEESQLGLLARCEACGAQLAQPLLSLQGWSGCMCSQGGGRWAVSGPLWLGPL
QSPDVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVYCWSTAELAKRLALSGPPSVLALVKALRSAGYEACCSAVMA
GQLRTDATVTELLRICHEMCQEGR

Sequences:

>Translated_424_residues
MGFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSVLLAAFQQQRREKFLPALHWL
DLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADLERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCR
FDLIDLDCFGCPNAFLQPVLQVLASEGLLLLASSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
GRGLEPLVSFSEGRTFRVAVRIKRRLAAGEESQLGLLARCEACGAQLAQPLLSLQGWSGCMCSQGGGRWAVSGPLWLGPL
QSPDVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVYCWSTAELAKRLALSGPPSVLALVKALRSAGYEACCSAVMA
GQLRTDATVTELLRICHEMCQEGR
>Mature_423_residues
GFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSVLLAAFQQQRREKFLPALHWLD
LMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADLERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCRF
DLIDLDCFGCPNAFLQPVLQVLASEGLLLLASSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLLG
RGLEPLVSFSEGRTFRVAVRIKRRLAAGEESQLGLLARCEACGAQLAQPLLSLQGWSGCMCSQGGGRWAVSGPLWLGPLQ
SPDVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVYCWSTAELAKRLALSGPPSVLALVKALRSAGYEACCSAVMAG
QLRTDATVTELLRICHEMCQEGR

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups [H]

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family [H]

Homologues:

Organism=Homo sapiens, GI8923219, Length=359, Percent_Identity=26.4623955431755, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI209862871, Length=359, Percent_Identity=26.4623955431755, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI216548445, Length=342, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320325, Length=363, Percent_Identity=24.2424242424242, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921190, Length=435, Percent_Identity=24.1379310344828, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022923
- InterPro:   IPR002905 [H]

Pfam domain/function: PF02005 TRM [H]

EC number: =2.1.1.32 [H]

Molecular weight: Translated: 45922; Mature: 45791

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSV
CCCCHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHH
LLAAFQQQRREKFLPALHWLDLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCRFDLIDLDCFGCPNAFLQPVL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
QVLASEGLLLLASSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
HHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHH
GRGLEPLVSFSEGRTFRVAVRIKRRLAAGEESQLGLLARCEACGAQLAQPLLSLQGWSGC
HCCCHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
MCSQGGGRWAVSGPLWLGPLQSPDVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVY
EECCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
CWSTAELAKRLALSGPPSVLALVKALRSAGYEACCSAVMAGQLRTDATVTELLRICHEMC
ECCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
QEGR
HCCC
>Mature Secondary Structure 
GFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSV
CCCHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHH
LLAAFQQQRREKFLPALHWLDLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCRFDLIDLDCFGCPNAFLQPVL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
QVLASEGLLLLASSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
HHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHH
GRGLEPLVSFSEGRTFRVAVRIKRRLAAGEESQLGLLARCEACGAQLAQPLLSLQGWSGC
HCCCHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
MCSQGGGRWAVSGPLWLGPLQSPDVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVY
EECCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
CWSTAELAKRLALSGPPSVLALVKALRSAGYEACCSAVMAGQLRTDATVTELLRICHEMC
ECCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
QEGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9685492; 10438627 [H]