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The map label for this gene is gltX

Identifier: 124022388

GI number: 124022388

Start: 636428

End: 637858

Strand: Reverse

Name: gltX

Synonym: P9303_06791

Alternate gene names: 124022388

Gene position: 637858-636428 (Counterclockwise)

Preceding gene: 124022390

Following gene: 124022383

Centisome position: 23.78

GC content: 54.16

Gene sequence:

>1431_bases
GTGAAGGTGCGCGTTCGGCTGGCCCCGAGTCCAACAGGCACACTTCACATCGGCACGGCAAGAACGGCAGTTTTCAACTG
GCTCTTTGCACGCCATCAGGGGGGAGAATTCCTCGTGCGCATCGAGGACACCGACAAAGAACGATCCAAGCCTGAATTCA
CGACCAACATCCTCGAAGGTCTGAAGTGGCTTGGCCTCAACTGGGATGAACAGCCCATCATTCAAAGCCAGCATGTGGAT
GACCATCGCGCAGCAATCCAAAAGCTGCTCGATCTTGACCTGGCCTACCGCTGCTACGCCAGCGAGACTGAACTGGAGGC
CATGCGAGAAACACAAAAGGCCCAGGGCCAGGCCCCTCGCTACGACAACCGTCACCGAAATCTCAATCCCGAGCAAGAAG
CCGCCTTCCAATCTGAAGGCAGAAGTGCCGTGGTGCGCTTCCGTATCGATGATGTTGCGGCCATCAGTTGGAAGGATCTG
GTGCGCGGTCCAATGCACTGGAGGGGATCAGATTTGGGCGGCGATATGGTGATCTCACGTCGGGCTCCAGCCAAGGAAAT
TGGCGACCCGCTCTATAACCTTGTGGTGGTGGTTGATGACGCCGCCATGAGTATCTCGCATGTGATCCGCGGCGAAGATC
ACATCGCAAACACGGCCAAACAACTGCTGATCTATGAGGCCCTTGGATTACCAATACCTCAATTCGCCCATACCCCGCTC
ATTCTCAATTCTGAAGGACGCAAACTCTCCAAGCGGGATGGAGTGACCTCGATCAGTGACTTCCAAGCCATGGGATATAC
AGCGGAAGCTATGGCCAACTACATGACCCTGTTGGGCTGGTCAGTCCCCGAAGGGACTGACGAACGGTTCACTCTTCAGC
AAGCTGCAACCGTCTTCAGTTTTGACCGAGTCAATAAAGCAGGAGCAAAGTTTGACTGGGACAAACTCAACTGGCTCAAC
TCTCAAGTGCTACATGACTTGCCTAAAGATCAACTGTTGCATGAACTCAAACCCTTGTGGTCTGAGGCTGGCTGGGCTCT
GCCAGAAGAAAACTGGTGTTTGGATCTAGCCGAGCTGCTTGGTCCATCGCTGACACTGCTCAAGGATGGTGTCGATCAAG
CCCGCCCTTTTTTCGAAGAACCAACGCTGCAAGCTGATGGCCTGGAACAACTTGCCATGGAAGGAGCCAAGGCAGGCCTT
AGCAATCTTCTCGATCAGCTGGATAGCACTTCCTGGGACGGTTTTGATGTCAAGCAAGCACAACAACTGCTAACGAATGC
TGCTCAGGCTGCCAACGTCAAAAAAGGTGTGATCATGAAAAGCCTCAGAGCTGCGCTTCTAGGCCGCCTGCAAGGGCCAG
ACCTAATCACAACATGGGGATTACTGGCCAGGATCGGGCAAGACTTGAACAGGCTGCGTCGTTGTCTCTGA

Upstream 100 bases:

>100_bases
GGACCGTAGTTCAATTGGTTAGAGCACCGCCCTGTCACGGCGGAAGTTGCGGGTTCGAACCCCGTCGGTCCCGTTCTTAT
TCCTAATCCTGAGAGTCATT

Downstream 100 bases:

>100_bases
GCAGTCGTCTTCGTTTGAATCTTCAGGAGCGATTAAACCCAGGGCCTTGGCCAGCGGCTGGGCCGTGAGGCCTTGAATGC
CAACCGTCATAAGGATGGTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGEFLVRIEDTDKERSKPEFTTNILEGLKWLGLNWDEQPIIQSQHVD
DHRAAIQKLLDLDLAYRCYASETELEAMRETQKAQGQAPRYDNRHRNLNPEQEAAFQSEGRSAVVRFRIDDVAAISWKDL
VRGPMHWRGSDLGGDMVISRRAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMTLLGWSVPEGTDERFTLQQAATVFSFDRVNKAGAKFDWDKLNWLN
SQVLHDLPKDQLLHELKPLWSEAGWALPEENWCLDLAELLGPSLTLLKDGVDQARPFFEEPTLQADGLEQLAMEGAKAGL
SNLLDQLDSTSWDGFDVKQAQQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL

Sequences:

>Translated_476_residues
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGEFLVRIEDTDKERSKPEFTTNILEGLKWLGLNWDEQPIIQSQHVD
DHRAAIQKLLDLDLAYRCYASETELEAMRETQKAQGQAPRYDNRHRNLNPEQEAAFQSEGRSAVVRFRIDDVAAISWKDL
VRGPMHWRGSDLGGDMVISRRAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMTLLGWSVPEGTDERFTLQQAATVFSFDRVNKAGAKFDWDKLNWLN
SQVLHDLPKDQLLHELKPLWSEAGWALPEENWCLDLAELLGPSLTLLKDGVDQARPFFEEPTLQADGLEQLAMEGAKAGL
SNLLDQLDSTSWDGFDVKQAQQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL
>Mature_476_residues
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGEFLVRIEDTDKERSKPEFTTNILEGLKWLGLNWDEQPIIQSQHVD
DHRAAIQKLLDLDLAYRCYASETELEAMRETQKAQGQAPRYDNRHRNLNPEQEAAFQSEGRSAVVRFRIDDVAAISWKDL
VRGPMHWRGSDLGGDMVISRRAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMTLLGWSVPEGTDERFTLQQAATVFSFDRVNKAGAKFDWDKLNWLN
SQVLHDLPKDQLLHELKPLWSEAGWALPEENWCLDLAELLGPSLTLLKDGVDQARPFFEEPTLQADGLEQLAMEGAKAGL
SNLLDQLDSTSWDGFDVKQAQQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=356, Percent_Identity=38.4831460674157, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI62241042, Length=343, Percent_Identity=24.198250728863, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=37.9381443298969, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI226510925, Length=275, Percent_Identity=32.7272727272727, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786895, Length=318, Percent_Identity=22.3270440251572, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=299, Percent_Identity=36.7892976588629, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17511007, Length=358, Percent_Identity=24.0223463687151, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=493, Percent_Identity=30.2231237322515, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=27.0386266094421, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=135, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=34.1948310139165, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PROM3 (A2C7H0)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016695.1
- ProteinModelPortal:   A2C7H0
- SMR:   A2C7H0
- STRING:   A2C7H0
- GeneID:   4778974
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_06791
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   YRCFTSE
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53290; Mature: 53290

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGEFLVRIEDTDKERSKPEFTTNILEG
CEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LKWLGLNWDEQPIIQSQHVDDHRAAIQKLLDLDLAYRCYASETELEAMRETQKAQGQAPR
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
YDNRHRNLNPEQEAAFQSEGRSAVVRFRIDDVAAISWKDLVRGPMHWRGSDLGGDMVISR
CCCCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHCCHHHHHCCCCCCCCCCCCCCEEEEC
RAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
CCCHHHHCCHHEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMTLLGWSVPEGTDERFTLQQAATVFS
EECCCCCCCHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
FDRVNKAGAKFDWDKLNWLNSQVLHDLPKDQLLHELKPLWSEAGWALPEENWCLDLAELL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
GPSLTLLKDGVDQARPFFEEPTLQADGLEQLAMEGAKAGLSNLLDQLDSTSWDGFDVKQA
CCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
QQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGEFLVRIEDTDKERSKPEFTTNILEG
CEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LKWLGLNWDEQPIIQSQHVDDHRAAIQKLLDLDLAYRCYASETELEAMRETQKAQGQAPR
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
YDNRHRNLNPEQEAAFQSEGRSAVVRFRIDDVAAISWKDLVRGPMHWRGSDLGGDMVISR
CCCCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHCCHHHHHCCCCCCCCCCCCCCEEEEC
RAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
CCCHHHHCCHHEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMTLLGWSVPEGTDERFTLQQAATVFS
EECCCCCCCHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
FDRVNKAGAKFDWDKLNWLNSQVLHDLPKDQLLHELKPLWSEAGWALPEENWCLDLAELL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
GPSLTLLKDGVDQARPFFEEPTLQADGLEQLAMEGAKAGLSNLLDQLDSTSWDGFDVKQA
CCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
QQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA