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The map label for this gene is pilT [H]

Identifier: 124022383

GI number: 124022383

Start: 631993

End: 633264

Strand: Reverse

Name: pilT [H]

Synonym: P9303_06741

Alternate gene names: 124022383

Gene position: 633264-631993 (Counterclockwise)

Preceding gene: 124022388

Following gene: 124022382

Centisome position: 23.61

GC content: 53.46

Gene sequence:

>1272_bases
GTGCTTGCTGATCACCAAAAGTGCGCACGGACTTGCCAAGAGCTTCGCCGCGACGAAGCTGAAGAAAGCGGCCACAATCC
CCTCGTGAGTCAACCGGTCTTTCCGCCCAACTTCCCCCCTCAAGCACCAAAGTCAGCCTCTCAGTCGGCAGCACCGATAT
CCAGATCATCTACAGAGGGATTAACGCTATTCGCTGGAGCCACGCCTGGGGTCTCTCCCAACTTGGAGGAGATCGTACGA
ATCGCCTATCAAGAAGGACATTCCGATGTACACCTAGGGGTTGGCGAAGTGCCCCGTTTCCGAGACCGCGGCGAAATGCA
AAGCACAGATTGGCCTGTAACCGATCCAGAAAGATTTCAGGGTTGGTTGCAAGAGATCCTCTCCGCGAATCAGATCGATG
AATTCTTTAGAAGCAAAGAATTCGACGGCTCCCATGCCTTTCCCTTCGCAAGAGTCCGGATCAACCTGCTGGATTCCCTC
ACAGGGCCAGCGATGGTGCTTCGCATCATTCCCCAAACAATTCTCACCCTTGAGCAACTGCAACTCCCTGATGTCCTGAA
GGGTCTCTCATCCAAGCCGAAAGGACTTGTCTTGGTGACCGGCTCAACCGGGTCAGGGAAAAGCACCACCCTGGCGGCCA
TGATCGACTGGATCAATAGAAATCAACCCCGCCACATCCTCACCATCGAGGATCCTGTGGAGTTTGTACACAAGAGCAAG
CAATCCCTCGTCAAACATCGAGAACTGGGGAAACACACCCTGAAGTTCCACAACGCCCTGAAAGCAGCCCTACGAGAAGA
CCCAGATGTAATCCTGATCGGTGAGATCCGTGATCAAGAAACCCTGGCCACAGCGATTGAGGCCTCCCAAACGGGGCACC
TGGTGTTTGGCACTCTCCATACCAACTCGGCAGTCAAGACCATTGAGCGAGTCTTGGGCATGTATGCCCCAGAAGATCAA
GAGAGCATGCGTCGCTCCCTTTCAGAATCACTGCTGGGGGTGATTGCCCAAGGATTGATCCGAACAACCGACAACAAGCG
AGCTGCCTACCACGACATCCTGATTAATACCGATGCTTGCAAGGACTACATCCAAAGAGGAGCACTTGAGGATGTTGAGG
AGATCATGGAACGCAGCAGCTTTGATGGCATGATCACAGCCAATCAATCATTGCTGAATCTTGTCGATAATGGCCGGGTA
GAGCCAGAGATGGCAGTCGCCGCAAGCTTTAAGCCCAACGAACTAGCCCAAAGCATTCGTGGGCGGAATTGA

Upstream 100 bases:

>100_bases
TCACCGGTGCCAACTTGATCGCCTCTGGACGGGTGCCCAGCACGATCGTGACGCGGGGCAAACCAGCCATAAGAGAAGCA
ATCTTGAAGGGATCCTAAGA

Downstream 100 bases:

>100_bases
ACCACACCTAATTCAGCCAGGCTCTGCAGTCAGCAGGCATCACTACACGCAACAACAACAACACGCTGATCCCAATAATC
AATCCCGCCATTGCCATACG

Product: PilT2-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MLADHQKCARTCQELRRDEAEESGHNPLVSQPVFPPNFPPQAPKSASQSAAPISRSSTEGLTLFAGATPGVSPNLEEIVR
IAYQEGHSDVHLGVGEVPRFRDRGEMQSTDWPVTDPERFQGWLQEILSANQIDEFFRSKEFDGSHAFPFARVRINLLDSL
TGPAMVLRIIPQTILTLEQLQLPDVLKGLSSKPKGLVLVTGSTGSGKSTTLAAMIDWINRNQPRHILTIEDPVEFVHKSK
QSLVKHRELGKHTLKFHNALKAALREDPDVILIGEIRDQETLATAIEASQTGHLVFGTLHTNSAVKTIERVLGMYAPEDQ
ESMRRSLSESLLGVIAQGLIRTTDNKRAAYHDILINTDACKDYIQRGALEDVEEIMERSSFDGMITANQSLLNLVDNGRV
EPEMAVAASFKPNELAQSIRGRN

Sequences:

>Translated_423_residues
MLADHQKCARTCQELRRDEAEESGHNPLVSQPVFPPNFPPQAPKSASQSAAPISRSSTEGLTLFAGATPGVSPNLEEIVR
IAYQEGHSDVHLGVGEVPRFRDRGEMQSTDWPVTDPERFQGWLQEILSANQIDEFFRSKEFDGSHAFPFARVRINLLDSL
TGPAMVLRIIPQTILTLEQLQLPDVLKGLSSKPKGLVLVTGSTGSGKSTTLAAMIDWINRNQPRHILTIEDPVEFVHKSK
QSLVKHRELGKHTLKFHNALKAALREDPDVILIGEIRDQETLATAIEASQTGHLVFGTLHTNSAVKTIERVLGMYAPEDQ
ESMRRSLSESLLGVIAQGLIRTTDNKRAAYHDILINTDACKDYIQRGALEDVEEIMERSSFDGMITANQSLLNLVDNGRV
EPEMAVAASFKPNELAQSIRGRN
>Mature_423_residues
MLADHQKCARTCQELRRDEAEESGHNPLVSQPVFPPNFPPQAPKSASQSAAPISRSSTEGLTLFAGATPGVSPNLEEIVR
IAYQEGHSDVHLGVGEVPRFRDRGEMQSTDWPVTDPERFQGWLQEILSANQIDEFFRSKEFDGSHAFPFARVRINLLDSL
TGPAMVLRIIPQTILTLEQLQLPDVLKGLSSKPKGLVLVTGSTGSGKSTTLAAMIDWINRNQPRHILTIEDPVEFVHKSK
QSLVKHRELGKHTLKFHNALKAALREDPDVILIGEIRDQETLATAIEASQTGHLVFGTLHTNSAVKTIERVLGMYAPEDQ
ESMRRSLSESLLGVIAQGLIRTTDNKRAAYHDILINTDACKDYIQRGALEDVEEIMERSSFDGMITANQSLLNLVDNGRV
EPEMAVAASFKPNELAQSIRGRN

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=328, Percent_Identity=38.719512195122, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1789723, Length=277, Percent_Identity=37.5451263537906, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1786296, Length=196, Percent_Identity=43.3673469387755, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 46791; Mature: 46791

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADHQKCARTCQELRRDEAEESGHNPLVSQPVFPPNFPPQAPKSASQSAAPISRSSTEG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTLFAGATPGVSPNLEEIVRIAYQEGHSDVHLGVGEVPRFRDRGEMQSTDWPVTDPERFQ
EEEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
GWLQEILSANQIDEFFRSKEFDGSHAFPFARVRINLLDSLTGPAMVLRIIPQTILTLEQL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QLPDVLKGLSSKPKGLVLVTGSTGSGKSTTLAAMIDWINRNQPRHILTIEDPVEFVHKSK
CCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
QSLVKHRELGKHTLKFHNALKAALREDPDVILIGEIRDQETLATAIEASQTGHLVFGTLH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEC
TNSAVKTIERVLGMYAPEDQESMRRSLSESLLGVIAQGLIRTTDNKRAAYHDILINTDAC
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEECHHH
KDYIQRGALEDVEEIMERSSFDGMITANQSLLNLVDNGRVEPEMAVAASFKPNELAQSIR
HHHHHCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCHHEECCCCHHHHHHHHC
GRN
CCC
>Mature Secondary Structure
MLADHQKCARTCQELRRDEAEESGHNPLVSQPVFPPNFPPQAPKSASQSAAPISRSSTEG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTLFAGATPGVSPNLEEIVRIAYQEGHSDVHLGVGEVPRFRDRGEMQSTDWPVTDPERFQ
EEEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
GWLQEILSANQIDEFFRSKEFDGSHAFPFARVRINLLDSLTGPAMVLRIIPQTILTLEQL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QLPDVLKGLSSKPKGLVLVTGSTGSGKSTTLAAMIDWINRNQPRHILTIEDPVEFVHKSK
CCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
QSLVKHRELGKHTLKFHNALKAALREDPDVILIGEIRDQETLATAIEASQTGHLVFGTLH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEC
TNSAVKTIERVLGMYAPEDQESMRRSLSESLLGVIAQGLIRTTDNKRAAYHDILINTDAC
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEECHHH
KDYIQRGALEDVEEIMERSSFDGMITANQSLLNLVDNGRVEPEMAVAASFKPNELAQSIR
HHHHHCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCHHEECCCCHHHHHHHHC
GRN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]