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The map label for this gene is cobM [H]

Identifier: 124022371

GI number: 124022371

Start: 622948

End: 623703

Strand: Reverse

Name: cobM [H]

Synonym: P9303_06621

Alternate gene names: 124022371

Gene position: 623703-622948 (Counterclockwise)

Preceding gene: 124022372

Following gene: 124022370

Centisome position: 23.25

GC content: 55.42

Gene sequence:

>756_bases
ATGAGCAGAATCTCCATCGTTGGTGCAGGTCCAGGCGCGACTGATCTGCTAACACTGCGTGCCGCAGAACATCTCAAGCA
GGCTGAGGTCCTTGTATGGACCGATTCACTGGTTTCACCAGATATTGCAGCATTGGCACCTGAATCCTGTGAACGAATCC
GCACCAGCTCAATGACCCTCGAGGACGTTCTTCCGCTTCTGGTGAACAGGGCGCAAGCAGGCAAGCGGGTGGTCCGTCTT
CACGACGGAGACCCCTGCCTTTATGGGGCCTTGTCGGAACAGATCTGTGGCCTTGCTGACGCAGGCATTGAAGTAGAGGT
TGTGCCGGGTCTGAGTGCTTACCAAGCGACGGCCGCAGCCCTAAAAGCTGAACTCACCATCCCTGGCCTTGTCCAAACCA
TTGTTCTCAGCAGAGCTGGAGGACGCACCGGTGTTCCCGAGCGCGAAAACCTCCAACATCTGGCATCAATTGGAGCCTCT
CTATGCCTCTACCTCAGTGCACGCCATGTGGAGGAAGTACAAGCCACACTGCTGCAGCACTATTCAGCTGAAACACCAGT
AGCTATCGGCTACAGGGTGAGCTGGCCCGATCAATGGTTAAGCGTGGTGCCTTTGAAGCAAATGGCTACGACCTCGCGTG
AACACGAGCTGATTCGCACCACCCTTTATGTCGTCAGTCCGGCCCTAGCTGCAGGACGCCAACGTTCGAAGTTGTATTCT
CCAGACCACAAGCATCTCTTTCGCCAAAAGGGCTAA

Upstream 100 bases:

>100_bases
TGAGTTTGGCGATGATGGCAGTTGGAGGTTTTGGCCTCTGGTGGCTCTATGGTCGCAAACGAAAGCTACCGGACCCAGGT
AGGCCTAAGTCATTCGCTTG

Downstream 100 bases:

>100_bases
AGAGAGTGTTTTAAAATCCAGTAGTGCGGGCGATTAGCACAGTGGTAGCGCACTTCCTTCACACGGAAGGGGTCGTGGGT
TCGAATCCCGCATCGCCCAT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTLEDVLPLLVNRAQAGKRVVRL
HDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAALKAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASIGAS
LCLYLSARHVEEVQATLLQHYSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
PDHKHLFRQKG

Sequences:

>Translated_251_residues
MSRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTLEDVLPLLVNRAQAGKRVVRL
HDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAALKAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASIGAS
LCLYLSARHVEEVQATLLQHYSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
PDHKHLFRQKG
>Mature_250_residues
SRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTLEDVLPLLVNRAQAGKRVVRLH
DGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAALKAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASIGASL
CLYLSARHVEEVQATLLQHYSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYSP
DHKHLFRQKG

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=193, Percent_Identity=34.1968911917098, Blast_Score=98, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=218, Percent_Identity=27.9816513761468, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27085; Mature: 26954

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTL
CCCEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHCCHHHHHHHHHHCCCH
EDVLPLLVNRAQAGKRVVRLHDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAA
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
LKAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASIGASLCLYLSARHVEEVQATLLQH
HHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
HCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCC
PDHKHLFRQKG
CHHHHHHHCCC
>Mature Secondary Structure 
SRISIVGAGPGATDLLTLRAAEHLKQAEVLVWTDSLVSPDIAALAPESCERIRTSSMTL
CCEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHCCHHHHHHHHHHCCCH
EDVLPLLVNRAQAGKRVVRLHDGDPCLYGALSEQICGLADAGIEVEVVPGLSAYQATAAA
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
LKAELTIPGLVQTIVLSRAGGRTGVPERENLQHLASIGASLCLYLSARHVEEVQATLLQH
HHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSAETPVAIGYRVSWPDQWLSVVPLKQMATTSREHELIRTTLYVVSPALAAGRQRSKLYS
HCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCC
PDHKHLFRQKG
CHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]