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The map label for this gene is ymfI [H]

Identifier: 124021855

GI number: 124021855

Start: 156371

End: 157066

Strand: Direct

Name: ymfI [H]

Synonym: P9303_01421

Alternate gene names: 124021855

Gene position: 156371-157066 (Clockwise)

Preceding gene: 124021854

Following gene: 124021856

Centisome position: 5.83

GC content: 48.99

Gene sequence:

>696_bases
ATGAGTGGGAATGCTCTTCTGTTTGGGTCGCGCGGCGCTCTAGGCTCTGAAATTGAAAAGGTAATGAAGCAGAAAAGCTT
TCGTGTTTTGACTGCTGGCTCAGGGCCTGATGATGCTATCAACAATCATCTGCAGGTTGCCTATCAGCGGCCACAAGAGG
CAAGTGATTTTTATTCTTTGCCTTCTTTGGATGTCGTGATCTGGGCACATGGCTTGAACTGTAGCGATGTGATTGCTGAT
TTTGATTTAGAAGATCTAGAGCGTCTCTGGCAATCCAATGTGGTGTTTATTGCCTCAAGTCTATCTGCTTTACTGAAAGC
TGGAAAATTGCTTGCTGGTAGCCGCCTGGTTGTCGTCAGCTCGATTTGGCAGCAAGAAAGTCGTCCTGGAAAAATGTCTT
ACACGATTTCCAAGGCTGCCTTGCATGGCTTGGTCAAATCGTGTGCACTTGATCTTGGTGAGCGGGGCATCTTGATTAAT
GCTGTGCTGCCAGGAGTGGTTGATACGCCGATGACTCGCGCCAACTTGTCGTCTGAGCAGATTAAAGGGATTATTGATCA
GTCAGCACTCAGTCGCCTCGCTGAACCGCCAGACATCGCCTCTGCCGTAGCGTTCTTGGCGGACTCCTCCAACCGGGTGA
TCACAGGTCAGTTCCTTACAGTTGATGCTGGGTTCATTGGTATGAAGCATTCCTGA

Upstream 100 bases:

>100_bases
CAGGCCTATCAGAGGAAATGATTGATCGAGAATTGGCTTTGATTTCTGCTTTTGTTGAGGAGAAAAAATATAATATTAGC
CAACCCAATACTGCGATCTC

Downstream 100 bases:

>100_bases
GAACCGTTATGGCTGATCCGTTGACCCTCGATATCCAAAGCTCTTATGCCAATTACAAGGTTGCTTTTTCGGATCTCAAT
GATTTGAGCAAATGTTGCTT

Product: dehydrogenases with different specificities

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSGNALLFGSRGALGSEIEKVMKQKSFRVLTAGSGPDDAINNHLQVAYQRPQEASDFYSLPSLDVVIWAHGLNCSDVIAD
FDLEDLERLWQSNVVFIASSLSALLKAGKLLAGSRLVVVSSIWQQESRPGKMSYTISKAALHGLVKSCALDLGERGILIN
AVLPGVVDTPMTRANLSSEQIKGIIDQSALSRLAEPPDIASAVAFLADSSNRVITGQFLTVDAGFIGMKHS

Sequences:

>Translated_231_residues
MSGNALLFGSRGALGSEIEKVMKQKSFRVLTAGSGPDDAINNHLQVAYQRPQEASDFYSLPSLDVVIWAHGLNCSDVIAD
FDLEDLERLWQSNVVFIASSLSALLKAGKLLAGSRLVVVSSIWQQESRPGKMSYTISKAALHGLVKSCALDLGERGILIN
AVLPGVVDTPMTRANLSSEQIKGIIDQSALSRLAEPPDIASAVAFLADSSNRVITGQFLTVDAGFIGMKHS
>Mature_230_residues
SGNALLFGSRGALGSEIEKVMKQKSFRVLTAGSGPDDAINNHLQVAYQRPQEASDFYSLPSLDVVIWAHGLNCSDVIADF
DLEDLERLWQSNVVFIASSLSALLKAGKLLAGSRLVVVSSIWQQESRPGKMSYTISKAALHGLVKSCALDLGERGILINA
VLPGVVDTPMTRANLSSEQIKGIIDQSALSRLAEPPDIASAVAFLADSSNRVITGQFLTVDAGFIGMKHS

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=186, Percent_Identity=28.494623655914, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI40254992, Length=235, Percent_Identity=27.2340425531915, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI32483357, Length=148, Percent_Identity=31.7567567567568, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=163, Percent_Identity=31.2883435582822, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367365, Length=166, Percent_Identity=28.9156626506024, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788459, Length=172, Percent_Identity=30.8139534883721, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082100, Length=118, Percent_Identity=33.0508474576271, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787905, Length=149, Percent_Identity=31.5436241610738, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=166, Percent_Identity=33.7349397590361, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=113, Percent_Identity=36.283185840708, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644337, Length=167, Percent_Identity=32.3353293413174, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357041, Length=240, Percent_Identity=28.75, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644339, Length=171, Percent_Identity=30.9941520467836, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571526, Length=167, Percent_Identity=30.5389221556886, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24643142, Length=167, Percent_Identity=29.3413173652695, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI23397609, Length=178, Percent_Identity=29.2134831460674, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 24671; Mature: 24539

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNALLFGSRGALGSEIEKVMKQKSFRVLTAGSGPDDAINNHLQVAYQRPQEASDFYSL
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCHHHCCCEEEEECCCCCHHHHHCC
PSLDVVIWAHGLNCSDVIADFDLEDLERLWQSNVVFIASSLSALLKAGKLLAGSRLVVVS
CCCEEEEEECCCCHHHHHHCCCHHHHHHHHHCCEEEEHHHHHHHHHHCHHHCCCCCEEHH
SIWQQESRPGKMSYTISKAALHGLVKSCALDLGERGILINAVLPGVVDTPMTRANLSSEQ
HHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHCCCHHH
IKGIIDQSALSRLAEPPDIASAVAFLADSSNRVITGQFLTVDAGFIGMKHS
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCEEECCCC
>Mature Secondary Structure 
SGNALLFGSRGALGSEIEKVMKQKSFRVLTAGSGPDDAINNHLQVAYQRPQEASDFYSL
CCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCHHHCCCEEEEECCCCCHHHHHCC
PSLDVVIWAHGLNCSDVIADFDLEDLERLWQSNVVFIASSLSALLKAGKLLAGSRLVVVS
CCCEEEEEECCCCHHHHHHCCCHHHHHHHHHCCEEEEHHHHHHHHHHCHHHCCCCCEEHH
SIWQQESRPGKMSYTISKAALHGLVKSCALDLGERGILINAVLPGVVDTPMTRANLSSEQ
HHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHCCCHHH
IKGIIDQSALSRLAEPPDIASAVAFLADSSNRVITGQFLTVDAGFIGMKHS
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]