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The map label for this gene is rfbF [H]

Identifier: 124021852

GI number: 124021852

Start: 152924

End: 153691

Strand: Direct

Name: rfbF [H]

Synonym: P9303_01391

Alternate gene names: 124021852

Gene position: 152924-153691 (Clockwise)

Preceding gene: 124021851

Following gene: 124021853

Centisome position: 5.7

GC content: 48.96

Gene sequence:

>768_bases
ATGAAGGCCGTTATTCTTGCTGGTGGGCTCGGTACGAGGATTTCAGAGGAGACTCACCTCAAGCCAAAGCCGATGATTGA
GGTCGGAGGCCGCCCAATTCTTTGGCATATTCTAAAGATTTATTCGCAGCATGGCATCAATGATTTCGTGATTTGCTGTG
GATACAAAGGTTATGTGATTAAAGAATACTTTGCTAATTACTTTCTTCATAGTAGTGATGTCACCTTTGATATGAGCACG
AATCAAATGGAAGTGCATCAGCAGAATGCTGAGCCTTGGAAAGTCACCCTTGTTGATACTGGAGAATCAACTCTCACCGG
CGGTCGTTTGCGCAGGGTTGCTTCTTATTTAGATGGCGGACCTTTTTGCTTTACATACGGTGATGGAGTTGCTGATGTGG
ATTTAACGGCCTTGATTGCATTTCATCGCAATGAGGGTCGCTGGGCCACACTTACCGCTGTGCAGCCTCCTGGTCGCTTC
GGATCGCTTGCTTTTGAAAGGGGGCGGGTGCTGGGGTTTGAGGAGAAGCCACAGGGTGATGGATCTTGGATTAATGGTGG
CTTTTTTGTTCTGGAGCCAGAGGTTTTGGACACGATCAATGGCGACGAAAGCGTGTGGGAGAGGGATTGCTTGCCAGCTA
TCGCTTCTGCAGACCAACTCAATGCATTTCAGCACCATGGCTTCTGGCAACCCATGGATACCCTGCGCGATCGTCAGCTG
CTTGAAGAACTCTGGAAGGATGATCGCGCCCCATGGAAAACATGGTGA

Upstream 100 bases:

>100_bases
GGCCGAATTTGCTACGCTTTTTGTGAATATAATTTTAATATTGATTGCTTGCTATTGGCTGAAGCGCGTTCGCTACAATA
TGCCTTTTTGGGTCTAGGAA

Downstream 100 bases:

>100_bases
TGGCTGAGAGCTGGAATCCAGATTTTTGGTGTGGCAGGCGCGTCTGGCTCAGTGGCCACACAGGCTTTAAAGGCAGCTGG
TTGGCTCTTTGGTTGCTGCA

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKAVILAGGLGTRISEETHLKPKPMIEVGGRPILWHILKIYSQHGINDFVICCGYKGYVIKEYFANYFLHSSDVTFDMST
NQMEVHQQNAEPWKVTLVDTGESTLTGGRLRRVASYLDGGPFCFTYGDGVADVDLTALIAFHRNEGRWATLTAVQPPGRF
GSLAFERGRVLGFEEKPQGDGSWINGGFFVLEPEVLDTINGDESVWERDCLPAIASADQLNAFQHHGFWQPMDTLRDRQL
LEELWKDDRAPWKTW

Sequences:

>Translated_255_residues
MKAVILAGGLGTRISEETHLKPKPMIEVGGRPILWHILKIYSQHGINDFVICCGYKGYVIKEYFANYFLHSSDVTFDMST
NQMEVHQQNAEPWKVTLVDTGESTLTGGRLRRVASYLDGGPFCFTYGDGVADVDLTALIAFHRNEGRWATLTAVQPPGRF
GSLAFERGRVLGFEEKPQGDGSWINGGFFVLEPEVLDTINGDESVWERDCLPAIASADQLNAFQHHGFWQPMDTLRDRQL
LEELWKDDRAPWKTW
>Mature_255_residues
MKAVILAGGLGTRISEETHLKPKPMIEVGGRPILWHILKIYSQHGINDFVICCGYKGYVIKEYFANYFLHSSDVTFDMST
NQMEVHQQNAEPWKVTLVDTGESTLTGGRLRRVASYLDGGPFCFTYGDGVADVDLTALIAFHRNEGRWATLTAVQPPGRF
GSLAFERGRVLGFEEKPQGDGSWINGGFFVLEPEVLDTINGDESVWERDCLPAIASADQLNAFQHHGFWQPMDTLRDRQL
LEELWKDDRAPWKTW

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=246, Percent_Identity=28.4552845528455, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI11761619, Length=246, Percent_Identity=28.4552845528455, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI133931050, Length=253, Percent_Identity=28.8537549407115, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320148, Length=267, Percent_Identity=29.9625468164794, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 28797; Mature: 28797

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGLGTRISEETHLKPKPMIEVGGRPILWHILKIYSQHGINDFVICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHH
KEYFANYFLHSSDVTFDMSTNQMEVHQQNAEPWKVTLVDTGESTLTGGRLRRVASYLDGG
HHHHHHHEECCCCEEEEECCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
PFCFTYGDGVADVDLTALIAFHRNEGRWATLTAVQPPGRFGSLAFERGRVLGFEEKPQGD
CEEEECCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEECCCCEECCCCCCCCC
GSWINGGFFVLEPEVLDTINGDESVWERDCLPAIASADQLNAFQHHGFWQPMDTLRDRQL
CCEECCCEEEECHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHH
LEELWKDDRAPWKTW
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MKAVILAGGLGTRISEETHLKPKPMIEVGGRPILWHILKIYSQHGINDFVICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHH
KEYFANYFLHSSDVTFDMSTNQMEVHQQNAEPWKVTLVDTGESTLTGGRLRRVASYLDGG
HHHHHHHEECCCCEEEEECCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
PFCFTYGDGVADVDLTALIAFHRNEGRWATLTAVQPPGRFGSLAFERGRVLGFEEKPQGD
CEEEECCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEECCCCEECCCCCCCCC
GSWINGGFFVLEPEVLDTINGDESVWERDCLPAIASADQLNAFQHHGFWQPMDTLRDRQL
CCEECCCEEEECHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHH
LEELWKDDRAPWKTW
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]