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The map label for this gene is epsM [H]

Identifier: 124021824

GI number: 124021824

Start: 111474

End: 112070

Strand: Reverse

Name: epsM [H]

Synonym: P9303_01111

Alternate gene names: 124021824

Gene position: 112070-111474 (Counterclockwise)

Preceding gene: 124021825

Following gene: 124021823

Centisome position: 4.18

GC content: 34.84

Gene sequence:

>597_bases
ATGAAGAAATCTCTTCTATTAATTGGATGTGGAGGTCATGCAAAGTCTGTTATTGATCTAATCGAATCAAAAAATGATTT
CTATATTTACGGATTGATCGGTTTAGAGAATGATAGAGGAATTAACATAAACGACTATGGAGTGATAGGAGTCGATCAAG
ATTTACAACAATTAAGAAACGAAATTGACATGGCTTTTGTCGCTATAGGGCATTTTGGCAATACATGTAAGAGACGAATA
ATCACTAAGGTGATCAAGAACTACGATTATACATTGCCTACAATAATTTCACAACATTGCACAATAAGCAAGCGTGCGGT
AGTTGGTAATGGTACAAGTATAGGGCATGGATGTGTGATAAATTCTGGCGTTATTGTTGAGAATAGTTGTATTATTAATA
GTAAAACTCTTATAGAGCATGATTCAATAATCGGAGAACATAGTCATGTTAGTACAGGAGTAATTATTAATGGTAATGTA
GAAATAGGGAGTGATTGCTTTATAGGAAGCGGATGTATCATCCGTGAAGGCCTAAAAGTTCCTGATGGTACTATAATAAG
TGCAGGTACAAGAGTAATGGGATGGCCGTTAAGATAA

Upstream 100 bases:

>100_bases
AGCTTCCTATGTATATTGATGCCCCAAAAGGAAGTTTAATCGTAGCTGAGGGAGAAGAGAAAAGGTTAATAAATTTGCCA
AGTAGTCCTCAGTTATTGAA

Downstream 100 bases:

>100_bases
TAGCAGAGGCAGGTGTCAACCATAATGGCGAAATACAAAATGCATATCGACTAATAGATAGTGCGAAGTTTGCTGGTGCT
GACGCAATAAAGTTCCAAAG

Product: hypothetical protein

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKKSLLLIGCGGHAKSVIDLIESKNDFYIYGLIGLENDRGININDYGVIGVDQDLQQLRNEIDMAFVAIGHFGNTCKRRI
ITKVIKNYDYTLPTIISQHCTISKRAVVGNGTSIGHGCVINSGVIVENSCIINSKTLIEHDSIIGEHSHVSTGVIINGNV
EIGSDCFIGSGCIIREGLKVPDGTIISAGTRVMGWPLR

Sequences:

>Translated_198_residues
MKKSLLLIGCGGHAKSVIDLIESKNDFYIYGLIGLENDRGININDYGVIGVDQDLQQLRNEIDMAFVAIGHFGNTCKRRI
ITKVIKNYDYTLPTIISQHCTISKRAVVGNGTSIGHGCVINSGVIVENSCIINSKTLIEHDSIIGEHSHVSTGVIINGNV
EIGSDCFIGSGCIIREGLKVPDGTIISAGTRVMGWPLR
>Mature_198_residues
MKKSLLLIGCGGHAKSVIDLIESKNDFYIYGLIGLENDRGININDYGVIGVDQDLQQLRNEIDMAFVAIGHFGNTCKRRI
ITKVIKNYDYTLPTIISQHCTISKRAVVGNGTSIGHGCVINSGVIVENSCIINSKTLIEHDSIIGEHSHVSTGVIINGNV
EIGSDCFIGSGCIIREGLKVPDGTIISAGTRVMGWPLR

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 21367; Mature: 21367

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLLIGCGGHAKSVIDLIESKNDFYIYGLIGLENDRGININDYGVIGVDQDLQQLRN
CCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCEEEECCHHHHHHHH
EIDMAFVAIGHFGNTCKRRIITKVIKNYDYTLPTIISQHCTISKRAVVGNGTSIGHGCVI
HHHEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCEEE
NSGVIVENSCIINSKTLIEHDSIIGEHSHVSTGVIINGNVEIGSDCFIGSGCIIREGLKV
ECCEEEECCEEECCCCEEECCCCCCCCCCCEECEEEECCEEECCCEEECCCEEEECCCCC
PDGTIISAGTRVMGWPLR
CCCEEEECCCEEEECCCC
>Mature Secondary Structure
MKKSLLLIGCGGHAKSVIDLIESKNDFYIYGLIGLENDRGININDYGVIGVDQDLQQLRN
CCCCEEEEECCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCEEEECCHHHHHHHH
EIDMAFVAIGHFGNTCKRRIITKVIKNYDYTLPTIISQHCTISKRAVVGNGTSIGHGCVI
HHHEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCEEE
NSGVIVENSCIINSKTLIEHDSIIGEHSHVSTGVIINGNVEIGSDCFIGSGCIIREGLKV
ECCEEEECCEEECCCCEEECCCCCCCCCCCEECEEEECCEEECCCEEECCCEEEECCCCC
PDGTIISAGTRVMGWPLR
CCCEEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]