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The map label for this gene is epsN [H]

Identifier: 124021825

GI number: 124021825

Start: 112067

End: 113254

Strand: Reverse

Name: epsN [H]

Synonym: P9303_01121

Alternate gene names: 124021825

Gene position: 113254-112067 (Counterclockwise)

Preceding gene: 124021826

Following gene: 124021824

Centisome position: 4.22

GC content: 37.88

Gene sequence:

>1188_bases
ATGAAAAGTTTAGCCATTGAAATCGTAAATGCAATTGAGAAGGTTATTGGACCTGCTAGTAAAGATAATAAAATCTCGTT
ACATGAACCTGATTTTAACAAGACCAATGCTTGGAAGTATGTCAAAAATTGTATTGATAGTGGCTGGGTGAGTAGTACTG
GTAGTTGGGTTAATGAATTTGAAAAAGGAATTGTCAATTTAACTGGCGCAAAGTATGCAGTAGCTGTGGTTAACGGAACA
TCTGCGCTGAGATTAAGTTTACATGTAGTTGGGGTGAAGCCTAACGATGAAGTAATAATGTCACCTCTGAGTTTTGTGGC
AACAGCTAATTCTGTCAGCCACCTTGGGGCAATCCCTCATTTTGTAGATATTAATCGGCAAACTTTCGCATTAGACGCAA
CTACTCTCGACTTGCATTTAAACAAGATCGCAGTTAGGACTAACGGTGGTGTGATAAATACTATGACTGGTAACCGAATT
AGTGCTGTTGTGCCAGTTCATGTCTTCGGGCATGCTGCAGAAATGGAAGCAATCAAAAGTGTTACAGACTATTGGGGATT
AGCTTTAGTAGAAGATGCAGCAGAAGCATTGGGGAGTTGGAGAAATGATATACATTGTGGTTTATTTGGTGATATGGGGA
TTATAAGTTTTAATGGAAATAAAATAATAACTACAGGTGGGGGAGGTATGATTTTAACGAATAATGAAAATCGTTATCAA
AAAGCAAGACATTTATCGACTACCGCAAAGATAAAGCATGACTGGGAGTTTATCCATGATGCTGTGGCATGGAACGACCG
AATGCCAAATATCAATGCTGCGTTAGGGCTCGCACAGCTAGAGGCTCTAGAATTAAGGCTTGAAGCGAAAAGAAATCTAG
CTAAATTGTATAAAGAAAGTTTTAGTGCAATAGAAGAAGTTGAATTCATTGAAGAAATGGAGAATTGTATTAGTAATAAC
TGGTTAATAACGGTGAGGCTTAAAATGGCTGGATCAACGGAGTCGGATCAAATCAGAGAGCAAATATTGAGTTACGCTCA
CAAATCTGGAATATTATTGAGGCCAGTTTGGAATTTATTAAATGAGCTTCCTATGTATATTGATGCCCCAAAAGGAAGTT
TAATCGTAGCTGAGGGAGAAGAGAAAAGGTTAATAAATTTGCCAAGTAGTCCTCAGTTATTGAAATGA

Upstream 100 bases:

>100_bases
ATGACGGTAAGGATGGTTTTAAAAGAGGGATCGCTCGTACAGTTGAATGGTTTACAAAGCCAAATAATCTTGCATTATAT
AAACTTGGTCAATATTCCAT

Downstream 100 bases:

>100_bases
AGAAATCTCTTCTATTAATTGGATGTGGAGGTCATGCAAAGTCTGTTATTGATCTAATCGAATCAAAAAATGATTTCTAT
ATTTACGGATTGATCGGTTT

Product: pyridoxal phosphate-dependent enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKSLAIEIVNAIEKVIGPASKDNKISLHEPDFNKTNAWKYVKNCIDSGWVSSTGSWVNEFEKGIVNLTGAKYAVAVVNGT
SALRLSLHVVGVKPNDEVIMSPLSFVATANSVSHLGAIPHFVDINRQTFALDATTLDLHLNKIAVRTNGGVINTMTGNRI
SAVVPVHVFGHAAEMEAIKSVTDYWGLALVEDAAEALGSWRNDIHCGLFGDMGIISFNGNKIITTGGGGMILTNNENRYQ
KARHLSTTAKIKHDWEFIHDAVAWNDRMPNINAALGLAQLEALELRLEAKRNLAKLYKESFSAIEEVEFIEEMENCISNN
WLITVRLKMAGSTESDQIREQILSYAHKSGILLRPVWNLLNELPMYIDAPKGSLIVAEGEEKRLINLPSSPQLLK

Sequences:

>Translated_395_residues
MKSLAIEIVNAIEKVIGPASKDNKISLHEPDFNKTNAWKYVKNCIDSGWVSSTGSWVNEFEKGIVNLTGAKYAVAVVNGT
SALRLSLHVVGVKPNDEVIMSPLSFVATANSVSHLGAIPHFVDINRQTFALDATTLDLHLNKIAVRTNGGVINTMTGNRI
SAVVPVHVFGHAAEMEAIKSVTDYWGLALVEDAAEALGSWRNDIHCGLFGDMGIISFNGNKIITTGGGGMILTNNENRYQ
KARHLSTTAKIKHDWEFIHDAVAWNDRMPNINAALGLAQLEALELRLEAKRNLAKLYKESFSAIEEVEFIEEMENCISNN
WLITVRLKMAGSTESDQIREQILSYAHKSGILLRPVWNLLNELPMYIDAPKGSLIVAEGEEKRLINLPSSPQLLK
>Mature_395_residues
MKSLAIEIVNAIEKVIGPASKDNKISLHEPDFNKTNAWKYVKNCIDSGWVSSTGSWVNEFEKGIVNLTGAKYAVAVVNGT
SALRLSLHVVGVKPNDEVIMSPLSFVATANSVSHLGAIPHFVDINRQTFALDATTLDLHLNKIAVRTNGGVINTMTGNRI
SAVVPVHVFGHAAEMEAIKSVTDYWGLALVEDAAEALGSWRNDIHCGLFGDMGIISFNGNKIITTGGGGMILTNNENRYQ
KARHLSTTAKIKHDWEFIHDAVAWNDRMPNINAALGLAQLEALELRLEAKRNLAKLYKESFSAIEEVEFIEEMENCISNN
WLITVRLKMAGSTESDQIREQILSYAHKSGILLRPVWNLLNELPMYIDAPKGSLIVAEGEEKRLINLPSSPQLLK

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=255, Percent_Identity=32.5490196078431, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI2367285, Length=301, Percent_Identity=25.2491694352159, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 43465; Mature: 43465

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLAIEIVNAIEKVIGPASKDNKISLHEPDFNKTNAWKYVKNCIDSGWVSSTGSWVNEF
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
EKGIVNLTGAKYAVAVVNGTSALRLSLHVVGVKPNDEVIMSPLSFVATANSVSHLGAIPH
HCCCEEECCCEEEEEEEECCCEEEEEEEEEEECCCCHHEECCHHHHHHHCCHHHHCCCCC
FVDINRQTFALDATTLDLHLNKIAVRTNGGVINTMTGNRISAVVPVHVFGHAAEMEAIKS
EEECCCEEEEEEEEEEEEEEEEEEEEECCCEEECCCCCEEEEEEEEEECCCHHHHHHHHH
VTDYWGLALVEDAAEALGSWRNDIHCGLFGDMGIISFNGNKIITTGGGGMILTNNENRYQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCEEEEECCCEEEEECCCHHHH
KARHLSTTAKIKHDWEFIHDAVAWNDRMPNINAALGLAQLEALELRLEAKRNLAKLYKES
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSAIEEVEFIEEMENCISNNWLITVRLKMAGSTESDQIREQILSYAHKSGILLRPVWNLL
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEHHHHHHHH
NELPMYIDAPKGSLIVAEGEEKRLINLPSSPQLLK
HHCCCEEECCCCCEEEEECCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKSLAIEIVNAIEKVIGPASKDNKISLHEPDFNKTNAWKYVKNCIDSGWVSSTGSWVNEF
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
EKGIVNLTGAKYAVAVVNGTSALRLSLHVVGVKPNDEVIMSPLSFVATANSVSHLGAIPH
HCCCEEECCCEEEEEEEECCCEEEEEEEEEEECCCCHHEECCHHHHHHHCCHHHHCCCCC
FVDINRQTFALDATTLDLHLNKIAVRTNGGVINTMTGNRISAVVPVHVFGHAAEMEAIKS
EEECCCEEEEEEEEEEEEEEEEEEEEECCCEEECCCCCEEEEEEEEEECCCHHHHHHHHH
VTDYWGLALVEDAAEALGSWRNDIHCGLFGDMGIISFNGNKIITTGGGGMILTNNENRYQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCEEEEECCCEEEEECCCHHHH
KARHLSTTAKIKHDWEFIHDAVAWNDRMPNINAALGLAQLEALELRLEAKRNLAKLYKES
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSAIEEVEFIEEMENCISNNWLITVRLKMAGSTESDQIREQILSYAHKSGILLRPVWNLL
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEHHHHHHHH
NELPMYIDAPKGSLIVAEGEEKRLINLPSSPQLLK
HHCCCEEECCCCCEEEEECCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]