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The map label for this gene is rffG [C]

Identifier: 124021814

GI number: 124021814

Start: 100911

End: 101885

Strand: Direct

Name: rffG [C]

Synonym: P9303_01011

Alternate gene names: 124021814

Gene position: 100911-101885 (Clockwise)

Preceding gene: 124021813

Following gene: 124021815

Centisome position: 3.76

GC content: 40.62

Gene sequence:

>975_bases
GTGAAAGCAGTCGTCACAGGAGGCGCAGGTTTTATAGGCAGTCACTTGGTTGAACAGTTGCTCGAACTTGGCCATTCTGT
ATCTATCATAGACAACTACTCAACAGGAAGGCCAGAAAATATAAGCCATGTAATCAATCAAGTCACTGTAGATCAGAGCG
ACCTTTCCATTAGGGGTTCATGGGAGCATTTGATTAGAGAATCGGATATTATTTTTCATTTGGCAAGCCTCGCGGACATT
GTTCCAAGCATTGAAAATCCACAGCTTTACTTTCACTCAAATGTTACATCAACTGTTAACATTATGGAAGTTGCAAAAGA
GCGCAAGAATAGAGTTATTTATGCGGCCTCTTCTTCTTGTTATGGTATCCCAGATAGTTACCCAACCGCAGAGAATGCTG
AAATTAGGCCCGAGTATCCTTATGCATTGACTAAATGGTTAGGAGAACAAATAGTAATACATTGGGGTAAGATATATAAT
ATACCTGTGATCTCAACACGCTTCTTTAATGTATATGGAACACGTTCCAGAACTTCAGGTACATATGGTGCAGTTTTTGG
TGTATTCTTAGCACAGAAACTTGCTAACAAGCCTCTCACAATTGTTGGCAATGGAGAGCAGAAAAGAGATTTTACTTATG
TTACAGATGTTTGCAACGGCCTTATTAAAGCAGCGCTAAGCTCTGTTACTAACTCAATTATTAACATTGGGTCAGGCAAT
CCTCAGACCATAAACTATTTAGCAAATCTGATTGGAGGCGAGCGTGTTTTTATTCCCCAAAGACCAGGGGAACCGGATAT
TACCCATGCAGATATAACCCTGGCTCGCGAGTTGTTAGGCTATTCTCCAAAAATTACCTTTGAAGAAGGTGTTCAAAAAG
TAATGGATTCAATCTCATATTGGTCAGAGGCACCAGTGTGGACACCAGAATCGATATCTCGAGCAACAGATTCTTGGTTT
AAGCACTTGTCGTGA

Upstream 100 bases:

>100_bases
CAATAGAGTATTCTCTGATGTGATGTAAGCATAAGTCTGAATCATCTCTCAGTATTATTGATCATTAGGTTATAATCATA
GATTAATCGAATCAAACATC

Downstream 100 bases:

>100_bases
TATAATAGAACTTTACTAATTGATCAAGCATGACTGATAAGTATTTGATAGATAGTCATAAATATGTATTTCATCCTGAG
CATTCAGCTAAACATTTACA

Product: nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKAVVTGGAGFIGSHLVEQLLELGHSVSIIDNYSTGRPENISHVINQVTVDQSDLSIRGSWEHLIRESDIIFHLASLADI
VPSIENPQLYFHSNVTSTVNIMEVAKERKNRVIYAASSSCYGIPDSYPTAENAEIRPEYPYALTKWLGEQIVIHWGKIYN
IPVISTRFFNVYGTRSRTSGTYGAVFGVFLAQKLANKPLTIVGNGEQKRDFTYVTDVCNGLIKAALSSVTNSIINIGSGN
PQTINYLANLIGGERVFIPQRPGEPDITHADITLARELLGYSPKITFEEGVQKVMDSISYWSEAPVWTPESISRATDSWF
KHLS

Sequences:

>Translated_324_residues
MKAVVTGGAGFIGSHLVEQLLELGHSVSIIDNYSTGRPENISHVINQVTVDQSDLSIRGSWEHLIRESDIIFHLASLADI
VPSIENPQLYFHSNVTSTVNIMEVAKERKNRVIYAASSSCYGIPDSYPTAENAEIRPEYPYALTKWLGEQIVIHWGKIYN
IPVISTRFFNVYGTRSRTSGTYGAVFGVFLAQKLANKPLTIVGNGEQKRDFTYVTDVCNGLIKAALSSVTNSIINIGSGN
PQTINYLANLIGGERVFIPQRPGEPDITHADITLARELLGYSPKITFEEGVQKVMDSISYWSEAPVWTPESISRATDSWF
KHLS
>Mature_324_residues
MKAVVTGGAGFIGSHLVEQLLELGHSVSIIDNYSTGRPENISHVINQVTVDQSDLSIRGSWEHLIRESDIIFHLASLADI
VPSIENPQLYFHSNVTSTVNIMEVAKERKNRVIYAASSSCYGIPDSYPTAENAEIRPEYPYALTKWLGEQIVIHWGKIYN
IPVISTRFFNVYGTRSRTSGTYGAVFGVFLAQKLANKPLTIVGNGEQKRDFTYVTDVCNGLIKAALSSVTNSIINIGSGN
PQTINYLANLIGGERVFIPQRPGEPDITHADITLARELLGYSPKITFEEGVQKVMDSISYWSEAPVWTPESISRATDSWF
KHLS

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=305, Percent_Identity=33.7704918032787, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI56237023, Length=347, Percent_Identity=25.0720461095101, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI56118217, Length=347, Percent_Identity=25.0720461095101, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI189083684, Length=347, Percent_Identity=25.0720461095101, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI7657641, Length=318, Percent_Identity=27.3584905660377, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI193211614, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI8393516, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=26.3610315186246, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=24.3093922651934, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=26.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788589, Length=336, Percent_Identity=23.8095238095238, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790049, Length=309, Percent_Identity=26.537216828479, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=23.6311239193084, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=307, Percent_Identity=33.5504885993485, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71982035, Length=334, Percent_Identity=29.0419161676647, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=336, Percent_Identity=28.8690476190476, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568069, Length=321, Percent_Identity=23.98753894081, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=24.8484848484848, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=252, Percent_Identity=28.1746031746032, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356223, Length=311, Percent_Identity=34.4051446945338, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 35853; Mature: 35853

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVVTGGAGFIGSHLVEQLLELGHSVSIIDNYSTGRPENISHVINQVTVDQSDLSIRGS
CCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEECCC
WEHLIRESDIIFHLASLADIVPSIENPQLYFHSNVTSTVNIMEVAKERKNRVIYAASSSC
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCC
YGIPDSYPTAENAEIRPEYPYALTKWLGEQIVIHWGKIYNIPVISTRFFNVYGTRSRTSG
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCEECCCEEEEEEEEEECCCCCCCC
TYGAVFGVFLAQKLANKPLTIVGNGEQKRDFTYVTDVCNGLIKAALSSVTNSIINIGSGN
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
PQTINYLANLIGGERVFIPQRPGEPDITHADITLARELLGYSPKITFEEGVQKVMDSISY
CHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
WSEAPVWTPESISRATDSWFKHLS
HCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAVVTGGAGFIGSHLVEQLLELGHSVSIIDNYSTGRPENISHVINQVTVDQSDLSIRGS
CCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEECCC
WEHLIRESDIIFHLASLADIVPSIENPQLYFHSNVTSTVNIMEVAKERKNRVIYAASSSC
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCC
YGIPDSYPTAENAEIRPEYPYALTKWLGEQIVIHWGKIYNIPVISTRFFNVYGTRSRTSG
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCEECCCEEEEEEEEEECCCCCCCC
TYGAVFGVFLAQKLANKPLTIVGNGEQKRDFTYVTDVCNGLIKAALSSVTNSIINIGSGN
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
PQTINYLANLIGGERVFIPQRPGEPDITHADITLARELLGYSPKITFEEGVQKVMDSISY
CHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
WSEAPVWTPESISRATDSWFKHLS
HCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]