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The map label for this gene is rfaE [H]

Identifier: 124021813

GI number: 124021813

Start: 99327

End: 100838

Strand: Direct

Name: rfaE [H]

Synonym: P9303_01001

Alternate gene names: 124021813

Gene position: 99327-100838 (Clockwise)

Preceding gene: 124021812

Following gene: 124021814

Centisome position: 3.7

GC content: 38.76

Gene sequence:

>1512_bases
ATGACTGAAAATATGACAGACACCATTAGCATTAGGGATATAGGAACAGTTCTTGACTCTGTTCTAGCTTATGGCCATTT
TAACTCTGTACACCCTGGTCATATCAGGCATCTAAAATCTGCTAAGGAAATAGGAACAAAGGTCGTTGTTGCGGTAATCG
GTGATCAATCAGATTCGGTTAAAAATTTCTTTTCACAGACCGAAAGAGCTGAGTCCTTAGCCCTTCTGAAATTAGCTGAC
ATCATCGTATTACTGGATGGTGAGGAACTTGATGTAGCGATTGAAAAAATAAGACCAAACACAGTACTTTTAGGGAAGGA
ACATCAAAGAACTACCAATAAGAACATCATGGAAGCTATAAAAACTCAGAAGCGATTAGGCGGGGTTGTTAAATATGATG
CAGGCGAAACACATTATGCAACTTCAGAACTATTGGGTTTTTCGCAAGCAGATATAAGAAATGAAAGAACTGCTAAGTTC
TTAGAAACATGTAGACGCCAATCCATTCAAGAAGATCAATTAATTGAAGCCACAAGGCTTTGGGCTAATACTAAAATAGT
GGTCATTGGAGATACGATTGTTGATCAATATGTGGCTTGTGAGGCTCTAGGCTTAAGTGCTGAAGCACCAGTAGTAGTAG
TAAGAGAGTTGGAAAATAAGAATTTTGTAGGAGGAGCAGCAATCGTTGCATCCCATATAAGAGCTCTTGGAGCACAATGC
CATTACATCTCTGTAGTGGGTAATGATCAAGAAGCCTCAGATGTAATCGAAATCCTTAAGGAAAGGGATATTAGCTTTAA
TCTAATTAAGGATAAAACACGACCAACAACATTTAAAAGGAGATATGTAGTTGAGAATCAAAAGCTATTCAGAGTGAGCA
GATTAGATGATAGGTGCTTGAGTCCAGAGAAGGAAGATGAGATAATACAAGCAATTGAGTATTCATTCCAATCAGCAAAT
GGGGTTGTAGTCTCTGACTTTGTCTATGGATTACTTACCCCTAGGATTATTAATCATATCCAAGTGATGGCAAATAAAGA
AAACATCATGCTCTTTGGTGATGTGCAATGTAGCAGTCAGGTTGGCAATATAGCCAAACTAAAAAATTTTACTCTCCTAT
GCCCAAATGAAAGGGAAGCAAGAATCGCACTGCAGGATAAGGATTCAGGCATTGAGAAGATTAGCCATAAGTTGATCACT
GCCACTAGAGCCGAAAAGTTAGTGATGAAGCTTGGTGCTCAAGGATTTATTGCTTATAATATTCAAAATGATGGCGAATT
AAAAAATGAACCTTTCCCAGCGCTCTCTGTAAACCCAGTTGATGTAACTGGAGCTGGAGATTCATTATTAGCGGTAATGG
CAATAGGATTAGCCAGTGGTCAAGAAATGATGCTTTGTTCCGCATTGGCTTGCTGTATGGCCTCTACAGCAGTTGATAAT
ATGGGCAACACGCCAATCACTAAAGCAGATCTACTAGAAAAGATCAATAGAGTATTCTCTGATGTGATGTAA

Upstream 100 bases:

>100_bases
TGCTAATGCAATTCTTAGTTGAAGCACGATCTAACATATTTAACCATGACTTTATATCCTCAGCTGTAAATACAGTAGAT
TCTTCAGCTAATATAAAAAA

Downstream 100 bases:

>100_bases
GCATAAGTCTGAATCATCTCTCAGTATTATTGATCATTAGGTTATAATCATAGATTAATCGAATCAAACATCGTGAAAGC
AGTCGTCACAGGAGGCGCAG

Product: ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTENMTDTISIRDIGTVLDSVLAYGHFNSVHPGHIRHLKSAKEIGTKVVVAVIGDQSDSVKNFFSQTERAESLALLKLAD
IIVLLDGEELDVAIEKIRPNTVLLGKEHQRTTNKNIMEAIKTQKRLGGVVKYDAGETHYATSELLGFSQADIRNERTAKF
LETCRRQSIQEDQLIEATRLWANTKIVVIGDTIVDQYVACEALGLSAEAPVVVVRELENKNFVGGAAIVASHIRALGAQC
HYISVVGNDQEASDVIEILKERDISFNLIKDKTRPTTFKRRYVVENQKLFRVSRLDDRCLSPEKEDEIIQAIEYSFQSAN
GVVVSDFVYGLLTPRIINHIQVMANKENIMLFGDVQCSSQVGNIAKLKNFTLLCPNEREARIALQDKDSGIEKISHKLIT
ATRAEKLVMKLGAQGFIAYNIQNDGELKNEPFPALSVNPVDVTGAGDSLLAVMAIGLASGQEMMLCSALACCMASTAVDN
MGNTPITKADLLEKINRVFSDVM

Sequences:

>Translated_503_residues
MTENMTDTISIRDIGTVLDSVLAYGHFNSVHPGHIRHLKSAKEIGTKVVVAVIGDQSDSVKNFFSQTERAESLALLKLAD
IIVLLDGEELDVAIEKIRPNTVLLGKEHQRTTNKNIMEAIKTQKRLGGVVKYDAGETHYATSELLGFSQADIRNERTAKF
LETCRRQSIQEDQLIEATRLWANTKIVVIGDTIVDQYVACEALGLSAEAPVVVVRELENKNFVGGAAIVASHIRALGAQC
HYISVVGNDQEASDVIEILKERDISFNLIKDKTRPTTFKRRYVVENQKLFRVSRLDDRCLSPEKEDEIIQAIEYSFQSAN
GVVVSDFVYGLLTPRIINHIQVMANKENIMLFGDVQCSSQVGNIAKLKNFTLLCPNEREARIALQDKDSGIEKISHKLIT
ATRAEKLVMKLGAQGFIAYNIQNDGELKNEPFPALSVNPVDVTGAGDSLLAVMAIGLASGQEMMLCSALACCMASTAVDN
MGNTPITKADLLEKINRVFSDVM
>Mature_502_residues
TENMTDTISIRDIGTVLDSVLAYGHFNSVHPGHIRHLKSAKEIGTKVVVAVIGDQSDSVKNFFSQTERAESLALLKLADI
IVLLDGEELDVAIEKIRPNTVLLGKEHQRTTNKNIMEAIKTQKRLGGVVKYDAGETHYATSELLGFSQADIRNERTAKFL
ETCRRQSIQEDQLIEATRLWANTKIVVIGDTIVDQYVACEALGLSAEAPVVVVRELENKNFVGGAAIVASHIRALGAQCH
YISVVGNDQEASDVIEILKERDISFNLIKDKTRPTTFKRRYVVENQKLFRVSRLDDRCLSPEKEDEIIQAIEYSFQSANG
VVVSDFVYGLLTPRIINHIQVMANKENIMLFGDVQCSSQVGNIAKLKNFTLLCPNEREARIALQDKDSGIEKISHKLITA
TRAEKLVMKLGAQGFIAYNIQNDGELKNEPFPALSVNPVDVTGAGDSLLAVMAIGLASGQEMMLCSALACCMASTAVDNM
GNTPITKADLLEKINRVFSDVM

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=309, Percent_Identity=26.2135922330097, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 55410; Mature: 55279

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENMTDTISIRDIGTVLDSVLAYGHFNSVHPGHIRHLKSAKEIGTKVVVAVIGDQSDSV
CCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHH
KNFFSQTERAESLALLKLADIIVLLDGEELDVAIEKIRPNTVLLGKEHQRTTNKNIMEAI
HHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
KTQKRLGGVVKYDAGETHYATSELLGFSQADIRNERTAKFLETCRRQSIQEDQLIEATRL
HHHHHHCCEEEECCCCCCCHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
WANTKIVVIGDTIVDQYVACEALGLSAEAPVVVVRELENKNFVGGAAIVASHIRALGAQC
HCCCEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCE
HYISVVGNDQEASDVIEILKERDISFNLIKDKTRPTTFKRRYVVENQKLFRVSRLDDRCL
EEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHEEECCCHHEEHHHCCHHHC
SPEKEDEIIQAIEYSFQSANGVVVSDFVYGLLTPRIINHIQVMANKENIMLFGDVQCSSQ
CCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCHH
VGNIAKLKNFTLLCPNEREARIALQDKDSGIEKISHKLITATRAEKLVMKLGAQGFIAYN
CCCHHHHCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IQNDGELKNEPFPALSVNPVDVTGAGDSLLAVMAIGLASGQEMMLCSALACCMASTAVDN
ECCCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
MGNTPITKADLLEKINRVFSDVM
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TENMTDTISIRDIGTVLDSVLAYGHFNSVHPGHIRHLKSAKEIGTKVVVAVIGDQSDSV
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHH
KNFFSQTERAESLALLKLADIIVLLDGEELDVAIEKIRPNTVLLGKEHQRTTNKNIMEAI
HHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
KTQKRLGGVVKYDAGETHYATSELLGFSQADIRNERTAKFLETCRRQSIQEDQLIEATRL
HHHHHHCCEEEECCCCCCCHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
WANTKIVVIGDTIVDQYVACEALGLSAEAPVVVVRELENKNFVGGAAIVASHIRALGAQC
HCCCEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCE
HYISVVGNDQEASDVIEILKERDISFNLIKDKTRPTTFKRRYVVENQKLFRVSRLDDRCL
EEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHEEECCCHHEEHHHCCHHHC
SPEKEDEIIQAIEYSFQSANGVVVSDFVYGLLTPRIINHIQVMANKENIMLFGDVQCSSQ
CCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCHH
VGNIAKLKNFTLLCPNEREARIALQDKDSGIEKISHKLITATRAEKLVMKLGAQGFIAYN
CCCHHHHCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IQNDGELKNEPFPALSVNPVDVTGAGDSLLAVMAIGLASGQEMMLCSALACCMASTAVDN
ECCCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
MGNTPITKADLLEKINRVFSDVM
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA