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The map label for this gene is apxIA [H]

Identifier: 124021808

GI number: 124021808

Start: 94830

End: 95951

Strand: Direct

Name: apxIA [H]

Synonym: P9303_00951

Alternate gene names: 124021808

Gene position: 94830-95951 (Clockwise)

Preceding gene: 124021807

Following gene: 124021809

Centisome position: 3.53

GC content: 48.31

Gene sequence:

>1122_bases
ATGAGGTCAATTGCGGCGAATGAGCTGATTGGGGAAAATCTTGCGTCTTTTACGCGGGGCCAGCTCAATCGTGATGGTTT
CATCGATATTTATTTGCATACTCCTGGCGGTGCGGTTTCGGTAGGTGGGGGGAGTGTCTCGAGCCAAACAATCTCAAGCC
TGCCAATCAGCGCTTCTGATCAACAGTTTTTCCAAGACATTGTGCTGTCCCTTGATCAGCGCTTGGACGCTGAGTTTCGC
TTTACTAGCGATAAAGAGGTTAGCGACATTCAATTGTATTATGATAGCGAAATCAACCTTGGTGGTGACATCCTGGGTCT
CGCCATGGCGAATAATGAGGCGGGTCGTGATTGGTGGGAGCTATTTATCAATAAACCGGCTTTTGGTGCTGATCTGAACC
ATTTGCGGTATTCCCTCATCCATGAATTGGGCCATGCTCTTGGGCTTGAGCATCCTTTTGATAACACTGATGGTGATGTC
GTCGATGGGATTACGGATCCATGGACCAGTGCCTATCCAGAAGATACTGTGATGGCCTATCGCTCTCCTTCTAAAGGGAG
TTGGCCGAATGCCTATACCGTTAATGATTTAGAGGCTTTGGTATCGATTTGGGGCGCTGAAACACAGGTGTTCTCTAATC
AGTCAGAACAGATTTTTGGTGAAGATTACAGTGAAGTAATCATCGCGGCTGGCGGGGACGATGCATTGTTTGGTGCTGGT
GGCAATGATGATCTCTTGGGTGGCATAGGTTTTGATCTTGTACGCGGTGGCCCTGGCGATGATGTTGTTCGAGGAGGCAA
GAATGCAGATCAGTTGTATGGAGGGAGAGGTAATGACCTCCTGAGGGGAGGGCGCGGACATGATCAGCTCAAAGGTGGAG
TTGGTGATGACCTCTATTGGGGTGGCCCTGGAGCCGATCTATTTCGCTTCAGCTCCGGCAATGACAGCGTGTTTGATTTC
AATATTGCTGATGCTGATTGCCTTGGCTTAGAAGCTGGTTTGAGTTATTCCATTGCTCAGCAGGGCAACGATCTGCTTGT
GCAGACCTCTCTTGGCACGATCACGCTAATGGGAATCAATATTTCTGAATTTGATGTCAATACTCAGATCATTATGATCT
AG

Upstream 100 bases:

>100_bases
ATGCTTGATCATGACCTAGCAAGTTATTATGTGCTTCTTATGTGAACTTAATCGGCAAATAGAACTTAAAACAGGTGGCT
ATAACTATTATAGGACTGCA

Downstream 100 bases:

>100_bases
TTTCGTTGCCTGTCTCTTCTATGTCTGTGCGGGTTTTATTAACCGGCTCTAAAGGGCAGTTGGGTCAGGAATTGATTCGC
TCTTGTCCATCAGATGTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: APX-IA; ApxI; Cytolysin IA; CLY-IA; Hemolysin IA; HLY-IA [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRSIAANELIGENLASFTRGQLNRDGFIDIYLHTPGGAVSVGGGSVSSQTISSLPISASDQQFFQDIVLSLDQRLDAEFR
FTSDKEVSDIQLYYDSEINLGGDILGLAMANNEAGRDWWELFINKPAFGADLNHLRYSLIHELGHALGLEHPFDNTDGDV
VDGITDPWTSAYPEDTVMAYRSPSKGSWPNAYTVNDLEALVSIWGAETQVFSNQSEQIFGEDYSEVIIAAGGDDALFGAG
GNDDLLGGIGFDLVRGGPGDDVVRGGKNADQLYGGRGNDLLRGGRGHDQLKGGVGDDLYWGGPGADLFRFSSGNDSVFDF
NIADADCLGLEAGLSYSIAQQGNDLLVQTSLGTITLMGINISEFDVNTQIIMI

Sequences:

>Translated_373_residues
MRSIAANELIGENLASFTRGQLNRDGFIDIYLHTPGGAVSVGGGSVSSQTISSLPISASDQQFFQDIVLSLDQRLDAEFR
FTSDKEVSDIQLYYDSEINLGGDILGLAMANNEAGRDWWELFINKPAFGADLNHLRYSLIHELGHALGLEHPFDNTDGDV
VDGITDPWTSAYPEDTVMAYRSPSKGSWPNAYTVNDLEALVSIWGAETQVFSNQSEQIFGEDYSEVIIAAGGDDALFGAG
GNDDLLGGIGFDLVRGGPGDDVVRGGKNADQLYGGRGNDLLRGGRGHDQLKGGVGDDLYWGGPGADLFRFSSGNDSVFDF
NIADADCLGLEAGLSYSIAQQGNDLLVQTSLGTITLMGINISEFDVNTQIIMI
>Mature_373_residues
MRSIAANELIGENLASFTRGQLNRDGFIDIYLHTPGGAVSVGGGSVSSQTISSLPISASDQQFFQDIVLSLDQRLDAEFR
FTSDKEVSDIQLYYDSEINLGGDILGLAMANNEAGRDWWELFINKPAFGADLNHLRYSLIHELGHALGLEHPFDNTDGDV
VDGITDPWTSAYPEDTVMAYRSPSKGSWPNAYTVNDLEALVSIWGAETQVFSNQSEQIFGEDYSEVIIAAGGDDALFGAG
GNDDLLGGIGFDLVRGGPGDDVVRGGKNADQLYGGRGNDLLRGGRGHDQLKGGVGDDLYWGGPGADLFRFSSGNDSVFDF
NIADADCLGLEAGLSYSIAQQGNDLLVQTSLGTITLMGINISEFDVNTQIIMI

Specific function: One of the virulence factors of A.pleuropneumoniae, which has a strong hemolytic activity and is cytotoxic for alveolar macrophages and neutrophils [H]

COG id: COG2931

COG function: function code Q; RTX toxins and related Ca2+-binding proteins

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 6 hemolysin-type calcium-binding repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018511
- InterPro:   IPR001343
- InterPro:   IPR013550
- InterPro:   IPR018504
- InterPro:   IPR003995
- InterPro:   IPR011049 [H]

Pfam domain/function: PF00353 HemolysinCabind; PF02382 RTX; PF08339 RTX_C [H]

EC number: NA

Molecular weight: Translated: 39958; Mature: 39958

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: PS00142 ZINC_PROTEASE ; PS00330 HEMOLYSIN_CALCIUM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIAANELIGENLASFTRGQLNRDGFIDIYLHTPGGAVSVGGGSVSSQTISSLPISASD
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHCCCCCCCH
QQFFQDIVLSLDQRLDAEFRFTSDKEVSDIQLYYDSEINLGGDILGLAMANNEAGRDWWE
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCHHHH
LFINKPAFGADLNHLRYSLIHELGHALGLEHPFDNTDGDVVDGITDPWTSAYPEDTVMAY
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEE
RSPSKGSWPNAYTVNDLEALVSIWGAETQVFSNQSEQIFGEDYSEVIIAAGGDDALFGAG
ECCCCCCCCCCEEHHHHHHHHHHHCCCHHHHCCCCCHHCCCCCCEEEEEECCCCEEEECC
GNDDLLGGIGFDLVRGGPGDDVVRGGKNADQLYGGRGNDLLRGGRGHDQLKGGVGDDLYW
CCCCCCCCCCHHEECCCCCHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCEE
GGPGADLFRFSSGNDSVFDFNIADADCLGLEAGLSYSIAQQGNDLLVQTSLGTITLMGIN
CCCCCCEEEEECCCCCEEEEECCCCHHEEHHCCCCEEEHHCCCEEEEEECCCEEEEEEEE
ISEFDVNTQIIMI
EEEECCCEEEEEC
>Mature Secondary Structure
MRSIAANELIGENLASFTRGQLNRDGFIDIYLHTPGGAVSVGGGSVSSQTISSLPISASD
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHCCCCCCCH
QQFFQDIVLSLDQRLDAEFRFTSDKEVSDIQLYYDSEINLGGDILGLAMANNEAGRDWWE
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCHHHH
LFINKPAFGADLNHLRYSLIHELGHALGLEHPFDNTDGDVVDGITDPWTSAYPEDTVMAY
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEE
RSPSKGSWPNAYTVNDLEALVSIWGAETQVFSNQSEQIFGEDYSEVIIAAGGDDALFGAG
ECCCCCCCCCCEEHHHHHHHHHHHCCCHHHHCCCCCHHCCCCCCEEEEEECCCCEEEECC
GNDDLLGGIGFDLVRGGPGDDVVRGGKNADQLYGGRGNDLLRGGRGHDQLKGGVGDDLYW
CCCCCCCCCCHHEECCCCCHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCEE
GGPGADLFRFSSGNDSVFDFNIADADCLGLEAGLSYSIAQQGNDLLVQTSLGTITLMGIN
CCCCCCEEEEECCCCCEEEEECCCCHHEEHHCCCCEEEHHCCCEEEEEECCCEEEEEEEE
ISEFDVNTQIIMI
EEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1879928; 8181764; 8359891 [H]