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The map label for this gene is rfbD [H]

Identifier: 124021809

GI number: 124021809

Start: 95972

End: 96907

Strand: Direct

Name: rfbD [H]

Synonym: P9303_00961

Alternate gene names: 124021809

Gene position: 95972-96907 (Clockwise)

Preceding gene: 124021808

Following gene: 124021810

Centisome position: 3.58

GC content: 51.18

Gene sequence:

>936_bases
ATGTCTGTGCGGGTTTTATTAACCGGCTCTAAAGGGCAGTTGGGTCAGGAATTGATTCGCTCTTGTCCATCAGATGTTGC
GTTGGTTGCTTGTGATCGTTCACAGTTAAATCTTGAACATGCCGATGCTTGTCAAGCCATCGTTGAAACACATCGGCCTG
ATTGGGTTATCAATGCAGGCGCTTATACAGCTGTTGATCGAGCAGAGCAAGAGCCACGGATTGCACAAGTTGTGAATGCT
GATGGGCCGAAGGCCTTGGCAGTGGGCCTGGCTCGTCATGGTGGGCGGATGTTGCAATTGAGTACTGATTTTGTCTTTGA
CGGCCAGCAGGGCACCCCATATCATCCCCAGCAATGTGTTTCGCCACTGGGTGTTTATGGCGCTAGCAAGGCAGCTGGTG
AAGAACACGTACTAAATGTTTTGGGTGATCGCGCACATGTGCTGCGAACCAGTTGGCTCTATGGACCGGTTGGTAGCAAT
TTTTTGCGCACGATGTTGAGGCTTCATCAGTCGAAAGCTGCGGCGGGGGAGCCATTGGCGGTGGTTTCTGATCAGGTTGG
CTGCCCTACCAGCACCTTGGGCCTGGCCGCAGCCTGTTGGCGATTGATTCAGACAGCTAGTCAAGTGCAAGATGAATGGA
TCCAAGAGCAGCAGCTGCCATCAATTCTTCATTGGAGTGATGCAGGAGCGGCAAGTTGGTATGACTTTGCTGTAGCTATT
GGTGAGCTGGGCGTTGCTGCAGGGATGCTTCAACATGCGGCGGATGTCAAACCCATTACAACTGCCGATTACCCCACTCC
AGCGAGGCGACCTAGTTACTCTTTGCTTGATTGCAGTGGTAGTTCGGCTGCTCTTGGACTCGAACCAATGTATTGGCGCA
ATGAATTGGAAGGAGTATTAGGAGAGTTGATTTCTGCACCTTGTACTTTTAGCTAA

Upstream 100 bases:

>100_bases
AGACCTCTCTTGGCACGATCACGCTAATGGGAATCAATATTTCTGAATTTGATGTCAATACTCAGATCATTATGATCTAG
TTTCGTTGCCTGTCTCTTCT

Downstream 100 bases:

>100_bases
GCTAATATCTTAACTGGTTTTTCTAGAGAATTAGACGGAGATCGGGTCGCCATCTTTGAAGAATCAATGAGATATTATAG
CACAATTATTTGATGTATTG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSVRVLLTGSKGQLGQELIRSCPSDVALVACDRSQLNLEHADACQAIVETHRPDWVINAGAYTAVDRAEQEPRIAQVVNA
DGPKALAVGLARHGGRMLQLSTDFVFDGQQGTPYHPQQCVSPLGVYGASKAAGEEHVLNVLGDRAHVLRTSWLYGPVGSN
FLRTMLRLHQSKAAAGEPLAVVSDQVGCPTSTLGLAAACWRLIQTASQVQDEWIQEQQLPSILHWSDAGAASWYDFAVAI
GELGVAAGMLQHAADVKPITTADYPTPARRPSYSLLDCSGSSAALGLEPMYWRNELEGVLGELISAPCTFS

Sequences:

>Translated_311_residues
MSVRVLLTGSKGQLGQELIRSCPSDVALVACDRSQLNLEHADACQAIVETHRPDWVINAGAYTAVDRAEQEPRIAQVVNA
DGPKALAVGLARHGGRMLQLSTDFVFDGQQGTPYHPQQCVSPLGVYGASKAAGEEHVLNVLGDRAHVLRTSWLYGPVGSN
FLRTMLRLHQSKAAAGEPLAVVSDQVGCPTSTLGLAAACWRLIQTASQVQDEWIQEQQLPSILHWSDAGAASWYDFAVAI
GELGVAAGMLQHAADVKPITTADYPTPARRPSYSLLDCSGSSAALGLEPMYWRNELEGVLGELISAPCTFS
>Mature_310_residues
SVRVLLTGSKGQLGQELIRSCPSDVALVACDRSQLNLEHADACQAIVETHRPDWVINAGAYTAVDRAEQEPRIAQVVNAD
GPKALAVGLARHGGRMLQLSTDFVFDGQQGTPYHPQQCVSPLGVYGASKAAGEEHVLNVLGDRAHVLRTSWLYGPVGSNF
LRTMLRLHQSKAAAGEPLAVVSDQVGCPTSTLGLAAACWRLIQTASQVQDEWIQEQQLPSILHWSDAGAASWYDFAVAIG
ELGVAAGMLQHAADVKPITTADYPTPARRPSYSLLDCSGSSAALGLEPMYWRNELEGVLGELISAPCTFS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=28.3216783216783, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=28.3216783216783, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1788352, Length=307, Percent_Identity=33.2247557003257, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33241; Mature: 33110

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRVLLTGSKGQLGQELIRSCPSDVALVACDRSQLNLEHADACQAIVETHRPDWVINAG
CCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECC
AYTAVDRAEQEPRIAQVVNADGPKALAVGLARHGGRMLQLSTDFVFDGQQGTPYHPQQCV
CHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCHHHHH
SPLGVYGASKAAGEEHVLNVLGDRAHVLRTSWLYGPVGSNFLRTMLRLHQSKAAAGEPLA
HHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCE
VVSDQVGCPTSTLGLAAACWRLIQTASQVQDEWIQEQQLPSILHWSDAGAASWYDFAVAI
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHH
GELGVAAGMLQHAADVKPITTADYPTPARRPSYSLLDCSGSSAALGLEPMYWRNELEGVL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
GELISAPCTFS
HHHHCCCCCCC
>Mature Secondary Structure 
SVRVLLTGSKGQLGQELIRSCPSDVALVACDRSQLNLEHADACQAIVETHRPDWVINAG
CEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECC
AYTAVDRAEQEPRIAQVVNADGPKALAVGLARHGGRMLQLSTDFVFDGQQGTPYHPQQCV
CHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCHHHHH
SPLGVYGASKAAGEEHVLNVLGDRAHVLRTSWLYGPVGSNFLRTMLRLHQSKAAAGEPLA
HHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCE
VVSDQVGCPTSTLGLAAACWRLIQTASQVQDEWIQEQQLPSILHWSDAGAASWYDFAVAI
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHH
GELGVAAGMLQHAADVKPITTADYPTPARRPSYSLLDCSGSSAALGLEPMYWRNELEGVL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
GELISAPCTFS
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]